Electrofocusing and Isotachophoresis : : Proceedings of the International Symposium, August 1-4, 1976, Hamburg, Germany / / B. J. Radola, D. Graesslin.

• TeilnehmendeR: Graesslin, D.,; Radola, B. J.,
• Place / Publishing House: Berlin ;, Boston : : De Gruyter, , [2019]; ©1977
• Year of Publication: 2019
• Edition: Reprint 2019
• Language: German
• Physical Description: 1 online resource (624 p.)

Table of Contents

• Frontmatter
• Preface
• Contents
• Contributors
• Part I. Isoelectric Focusing
• 1. Theoretical and General Aspects
• Reminiscences about the Genesis of Isoelectric Focusing and Generalization of the Idea / Kolin, A.
• Stable pH Gradients - A Key Problem in Isoelectric Focusing / Rilbe, H.
• Electrofocusing in Buffers: Formation of Natural pH Gradients, Flexibility, Gradient Stability, Relation to Isotachophoresis and Preparative Potential / Chrambach, A. / Nguyen, Nga Y.
• Comparison of pH Gradients Used for Isoelectric Focusing / Fawcett, J. S.
• On the Temperature Dependence of Isoelectric Points of Proteins with Special Reference to Isoelectric Focusing / Fredriksson, S.
• 2. Carrier Ampholytes
• Carrier Ampholyte Distribution / Brown, R. K. / Caspers, M. L. / Vinogradow, S. N.
• Characterization of Carrier Ampholytes by New Visualization Reactions and Chromatographic Methods / Radola, B. J. / Tschesche, H. / Schuricht, H.
• Synthesis of Carrier Ampholytes for Isoelectric Focusing Containing Sulfonic and Phosphonic Acid Groups Covering a Wide pH Range / Grubhofer, N. / Borja, C.
• 3. Analytical Methodology
• Staining of Proteins in Polyacrylamide Gels / Vesterberg, O. / Hansén, L.
• Isoelectric Focusing of Complex Protein Mixtures in the Nanogram Range and Enzyme Kinetics of Dehydrogenases Following IEF / Bispink, G. / Neuhoff, V.
• Soft Laser Scanning Densitometer Compatible with the High Resolution Obtained by Electrofocusing / Zeineh, R. A.
• Immunocore Electrofocusing: A Separation and Detection Technique Amenable to Scanning Densitometry / Zeineh, R. A.
• Rapid, Convenient and Economical Procedures for the Determination of the Isoelectric Spectra of Proteins / Leaback, D. H.
• A Simple Method of Choosing Optimum pH-Conditions for Electrophoresis / Rosengren, Å. / Bjellqvist, B. / Gasparic, V.
• pH Determinations in Isoelectric Focusing with an Iridium Electrode / Gianazza, E. / Righetti, P. G. / Bordi, S. / Papeschi, G.
• Anomalous Behaviour of Horseradish Peroxidase in Isoelectric Focusing / Delincée, H. / Radola, B. J.
• Does Lipoprotein Lipase Bind Ampholytes? / Bengtsson, G. / Olivecrona, T.
• 4. Biochemical Applications
• Zein: Macromolecular Properties, Biosynthesis and Genetic Regulation / Righetti, P. G. / Gianazza, E. / Salamini, F. / Galante, E. / Viotti, A. / Soave, C.
• Growth and Isoelectric Patterns of Peroxidase in Tobacco Tissue Cultures Under the Influence of Growth Regulator Systems / Rücker, W. / Markotai, J.
• A Method for Simultaneous Analysis of Several Genetic Loci in Mice After Electrofocusing / Narayanan, K. R. / Ray, A. S.
• Phylogenetic Differences in Isoelectric Components of Liver Acid Phosphatase and LDH Between Genera of Two Closely Related Rodent Families (Muridae and Microtidae) / Kubicz, A. / Wolanska, L.
• Fractionation of Nucleic Acids on Isoelectric Focusing / Drysdale, J. W.
• 5. Clinical Applications
• Polyacrylamide Gel Isoelectric Focusing of Alpha-1-Proteinase Inhibitors (α1 Pi) Phenotypes: I. Optimal Condition, Histochemical and Proteinase Probe Studies / Allen, R. C. / Oulla, P. M. / Arnaud, P. / Baumstark, J. S.
• Polyacrylamide Gel Isoelectric Focusing (PAGIF) Applied to Alpha-1-Proteinase Inhibitor (α1 Pi) Phenotyping: II. Immunochemical Procedures / Arnaud, P. / Chapuis-Cellier, C. / Wilson, G. B. / Koistinen, J. / Allen, R. C. / Fudenberg, H. H.
• High Resolution Phenotyping of α1-Antitrypsins by Thin-Layer Polyacrylamide Electrofocusing / Jeppsson, J. O.
• Broad-Beta Disease (Hyperlipoproteinaemia Type III): Genetics, Gene Frequency and Diagnosis without Ultracentrifugation / Utermann, G. / Hess, M. / Vogelberg, K. H.
• Screening for Abnormal Hemoglobins in the Newborn: A Highly Economic Procedure Using Isoelectric Focusing / Altland, K.
• The Crystallins of the Aging Lens from Five Species Studies by Various Methods of Thin-Layer Isoelectric Focusing / Bours, J.
• Separation of Erythrocyte Membrane Components in Electric Field. Polyacrylamide Gel Isoelectric Focusing: A Potential Method for the Diagnosis of Membrane Abnormality / Das, P. K. / Graesslin, D. / Goedde, H. W.
• Microheterogeneity of Serum Glycoproteins as Revealed by Flat-Bed Gel Isoelectric Focusing / Hamann, A.
• Diagnosis of Cystic Fibrosis (CF) - Purification and Distinction Between CF Protein and Ciliary Dyskinesia Activity in CF and Asthmatic Sera Using Isoelectric Focusing and the Rabbit Tracheal Ciliary Bioassay / Wilson, G. B. / Monsher, M. T. / Arnaud, P. / Fudenberg, H. H.
• Microheterogeneity of Human Kininogen, Alpha- 2-HS and Alpha-1-Acid Glycoproteins by Thin- Layer Isoelectric Focusing / Hamberg, U. / Turpeinen, U. / Knuutinen, U.
• A Genetic Variant of Amylase from Human Parotid Saliva Detected by Isoelectric Focusing / Pronk, J. C.
• 6. Two-Dimensional Techniques
• Biological Applications of Two-Dimensional Gel Electrophoresis / Ivarie, R. D. / Gelfand, D. H. / Jones, P. P. / O'Farrell, P. Z. / Polisky, B. A. / Steinberg, R. A. / O'Farrell, P. H.
• Plant Proteins Evaluated by Two-Dimensional Methods / Stegemann, H.
• Two-Dimensional Separation of Lymphocyte Microsomal Membrane Proteins: Isoelectric Focusing Linked to SDS-Polyacrylamide Gel Electrophoresis / Kniifermann, H.
• Mammalian Mitochondrial Ribosomes: Two- Dimensional Gel Electrophoresis of the 55 S- and the Corresponding Subunit Proteins / Czempiel, W.
• Crossed Immunoelectrofocusing for Standardization and Characterization of Fungal Antigens / Holmberg, K. / Wadström, T.
• 7. Preparative Separations
• Isoelectric Focusing in Free Water / Denckla, W. D.
• Preparative Electrofocusing in Flat-Beds of Granulated Gel - Methodological Aspects / Winter, A.
• Preparative Scale Purification of Bacterial Enzymes and Toxins by Isoelectric Focusing and Isotachophoresis / Wadstrom, T. / Möllby, R. / Olsson, B. / Söderholm, J. / Smyth, C. J.
• Preparative Zone Convection Electrofocusing in Helical Glass Tubes / Quast, R.
• Chromatofocusing: Isoelectric Focusing on Ion Exchangers in the Absence of an Externally Applied Potential / Sluyterman, L. A. A. E. / Wijdenes, J.
• 8. Separation of Cells
• New Instrumentation and Procedures for the Analysis of Mammalian Cells by Electrophoretic Techniques / Catsimpoolas, N. / Griffith, A. L.
• Continuous-Flow Isoelectric Focusing: Further Studies on Human Red Blood Cells and on the Separation of Rat Liver Light Mitochondrial Fraction / Just, W. W. / Werner, G.
• Preparative Electrofocusing of Native and Modified Living Mammalian Cells in a Stationary Ficoll/ Sucrose Gradient / Manske, W. / Bohn, B. / Brossmer, R.
• Part II. Isotachophoresis
• 1. Analytical and Preparative Methodology
• The Principle of Preparative Capillary Isotachophoresis / Arlinger, L.
• Preparative Capillary Isotachophoresis: Separation of μg Amounts of Some Human Serum Proteins / Moberg, U. / Hjalmarsson, S. G. / Arlinger, L. / Lundin, H.
• Quantitative Determination of Picomoles ADP by Means of Steady State Mixed Zones in Isotachophoresis / Wielders, J. P. M. / Everaerts, F. M.
• Thin-Layer Polyacrylamide Gel Isotachophoresis, Crossed Immuno-Isotachophoresis and Crossed Isotachophoresis-Electrofocusing / Brogren, C. H.
• Isotachophoresis of the Cerebrospinal Fluid (CSF) Proteins: Advantage of Prior Dialysis and its Practical Realization / Delmotte, P.
• 2. Applications
• Plasma Protein Fractionation in Sephadex by Isotachophoresis Using Discrete Spacers / Bier, M. / Kopwillem, A.
• Immuno-lsoelectric Focusing Analysis of Antibodies Fractionated by Isotachophoresis / Peltre, G. / Brogren, C. H.
• Analytical and Preparative Isotachophoresis in Column and Flat-Bed Gels for Fractionation of Antibodies / Brogren, C. H. / Peltre, G.
• Index
• Backmatter