Electrofocusing and Isotachophoresis : : Proceedings of the International Symposium, August 1-4, 1976, Hamburg, Germany / / B. J. Radola, D. Graesslin.
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Place / Publishing House: | Berlin ;, Boston : : De Gruyter, , [2019] ©1977 |
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Electrofocusing and Isotachophoresis : Proceedings of the International Symposium, August 1-4, 1976, Hamburg, Germany / B. J. Radola, D. Graesslin. Reprint 2019 Berlin ; Boston : De Gruyter, [2019] ©1977 1 online resource (624 p.) text txt rdacontent computer c rdamedia online resource cr rdacarrier text file PDF rda Frontmatter -- Preface -- Contents -- Contributors -- Part I. Isoelectric Focusing -- 1. Theoretical and General Aspects -- Reminiscences about the Genesis of Isoelectric Focusing and Generalization of the Idea / Kolin, A. -- Stable pH Gradients - A Key Problem in Isoelectric Focusing / Rilbe, H. -- Electrofocusing in Buffers: Formation of Natural pH Gradients, Flexibility, Gradient Stability, Relation to Isotachophoresis and Preparative Potential / Chrambach, A. / Nguyen, Nga Y. -- Comparison of pH Gradients Used for Isoelectric Focusing / Fawcett, J. S. -- On the Temperature Dependence of Isoelectric Points of Proteins with Special Reference to Isoelectric Focusing / Fredriksson, S. -- 2. Carrier Ampholytes -- Carrier Ampholyte Distribution / Brown, R. K. / Caspers, M. L. / Vinogradow, S. N. -- Characterization of Carrier Ampholytes by New Visualization Reactions and Chromatographic Methods / Radola, B. J. / Tschesche, H. / Schuricht, H. -- Synthesis of Carrier Ampholytes for Isoelectric Focusing Containing Sulfonic and Phosphonic Acid Groups Covering a Wide pH Range / Grubhofer, N. / Borja, C. -- 3. Analytical Methodology -- Staining of Proteins in Polyacrylamide Gels / Vesterberg, O. / Hansén, L. -- Isoelectric Focusing of Complex Protein Mixtures in the Nanogram Range and Enzyme Kinetics of Dehydrogenases Following IEF / Bispink, G. / Neuhoff, V. -- Soft Laser Scanning Densitometer Compatible with the High Resolution Obtained by Electrofocusing / Zeineh, R. A. -- Immunocore Electrofocusing: A Separation and Detection Technique Amenable to Scanning Densitometry / Zeineh, R. A. -- Rapid, Convenient and Economical Procedures for the Determination of the Isoelectric Spectra of Proteins / Leaback, D. H. -- A Simple Method of Choosing Optimum pH-Conditions for Electrophoresis / Rosengren, Å. / Bjellqvist, B. / Gasparic, V. -- pH Determinations in Isoelectric Focusing with an Iridium Electrode / Gianazza, E. / Righetti, P. G. / Bordi, S. / Papeschi, G. -- Anomalous Behaviour of Horseradish Peroxidase in Isoelectric Focusing / Delincée, H. / Radola, B. J. -- Does Lipoprotein Lipase Bind Ampholytes? / Bengtsson, G. / Olivecrona, T. -- 4. Biochemical Applications -- Zein: Macromolecular Properties, Biosynthesis and Genetic Regulation / Righetti, P. G. / Gianazza, E. / Salamini, F. / Galante, E. / Viotti, A. / Soave, C. -- Growth and Isoelectric Patterns of Peroxidase in Tobacco Tissue Cultures Under the Influence of Growth Regulator Systems / Rücker, W. / Markotai, J. -- A Method for Simultaneous Analysis of Several Genetic Loci in Mice After Electrofocusing / Narayanan, K. R. / Ray, A. S. -- Phylogenetic Differences in Isoelectric Components of Liver Acid Phosphatase and LDH Between Genera of Two Closely Related Rodent Families (Muridae and Microtidae) / Kubicz, A. / Wolanska, L. -- Fractionation of Nucleic Acids on Isoelectric Focusing / Drysdale, J. W. -- 5. Clinical Applications -- Polyacrylamide Gel Isoelectric Focusing of Alpha-1-Proteinase Inhibitors (α1 Pi) Phenotypes: I. Optimal Condition, Histochemical and Proteinase Probe Studies / Allen, R. C. / Oulla, P. M. / Arnaud, P. / Baumstark, J. S. -- Polyacrylamide Gel Isoelectric Focusing (PAGIF) Applied to Alpha-1-Proteinase Inhibitor (α1 Pi) Phenotyping: II. Immunochemical Procedures / Arnaud, P. / Chapuis-Cellier, C. / Wilson, G. B. / Koistinen, J. / Allen, R. C. / Fudenberg, H. H. -- High Resolution Phenotyping of α1-Antitrypsins by Thin-Layer Polyacrylamide Electrofocusing / Jeppsson, J. O. -- Broad-Beta Disease (Hyperlipoproteinaemia Type III): Genetics, Gene Frequency and Diagnosis without Ultracentrifugation / Utermann, G. / Hess, M. / Vogelberg, K. H. -- Screening for Abnormal Hemoglobins in the Newborn: A Highly Economic Procedure Using Isoelectric Focusing / Altland, K. -- The Crystallins of the Aging Lens from Five Species Studies by Various Methods of Thin-Layer Isoelectric Focusing / Bours, J. -- Separation of Erythrocyte Membrane Components in Electric Field. Polyacrylamide Gel Isoelectric Focusing: A Potential Method for the Diagnosis of Membrane Abnormality / Das, P. K. / Graesslin, D. / Goedde, H. W. -- Microheterogeneity of Serum Glycoproteins as Revealed by Flat-Bed Gel Isoelectric Focusing / Hamann, A. -- Diagnosis of Cystic Fibrosis (CF) - Purification and Distinction Between CF Protein and Ciliary Dyskinesia Activity in CF and Asthmatic Sera Using Isoelectric Focusing and the Rabbit Tracheal Ciliary Bioassay / Wilson, G. B. / Monsher, M. T. / Arnaud, P. / Fudenberg, H. H. -- Microheterogeneity of Human Kininogen, Alpha- 2-HS and Alpha-1-Acid Glycoproteins by Thin- Layer Isoelectric Focusing / Hamberg, U. / Turpeinen, U. / Knuutinen, U. -- A Genetic Variant of Amylase from Human Parotid Saliva Detected by Isoelectric Focusing / Pronk, J. C. -- 6. Two-Dimensional Techniques -- Biological Applications of Two-Dimensional Gel Electrophoresis / Ivarie, R. D. / Gelfand, D. H. / Jones, P. P. / O'Farrell, P. Z. / Polisky, B. A. / Steinberg, R. A. / O'Farrell, P. H. -- Plant Proteins Evaluated by Two-Dimensional Methods / Stegemann, H. -- Two-Dimensional Separation of Lymphocyte Microsomal Membrane Proteins: Isoelectric Focusing Linked to SDS-Polyacrylamide Gel Electrophoresis / Kniifermann, H. -- Mammalian Mitochondrial Ribosomes: Two- Dimensional Gel Electrophoresis of the 55 S- and the Corresponding Subunit Proteins / Czempiel, W. -- Crossed Immunoelectrofocusing for Standardization and Characterization of Fungal Antigens / Holmberg, K. / Wadström, T. -- 7. Preparative Separations -- Isoelectric Focusing in Free Water / Denckla, W. D. -- Preparative Electrofocusing in Flat-Beds of Granulated Gel - Methodological Aspects / Winter, A. -- Preparative Scale Purification of Bacterial Enzymes and Toxins by Isoelectric Focusing and Isotachophoresis / Wadstrom, T. / Möllby, R. / Olsson, B. / Söderholm, J. / Smyth, C. J. -- Preparative Zone Convection Electrofocusing in Helical Glass Tubes / Quast, R. -- Chromatofocusing: Isoelectric Focusing on Ion Exchangers in the Absence of an Externally Applied Potential / Sluyterman, L. A. A. E. / Wijdenes, J. -- 8. Separation of Cells -- New Instrumentation and Procedures for the Analysis of Mammalian Cells by Electrophoretic Techniques / Catsimpoolas, N. / Griffith, A. L. -- Continuous-Flow Isoelectric Focusing: Further Studies on Human Red Blood Cells and on the Separation of Rat Liver Light Mitochondrial Fraction / Just, W. W. / Werner, G. -- Preparative Electrofocusing of Native and Modified Living Mammalian Cells in a Stationary Ficoll/ Sucrose Gradient / Manske, W. / Bohn, B. / Brossmer, R. -- Part II. Isotachophoresis -- 1. Analytical and Preparative Methodology -- The Principle of Preparative Capillary Isotachophoresis / Arlinger, L. -- Preparative Capillary Isotachophoresis: Separation of μg Amounts of Some Human Serum Proteins / Moberg, U. / Hjalmarsson, S. G. / Arlinger, L. / Lundin, H. -- Quantitative Determination of Picomoles ADP by Means of Steady State Mixed Zones in Isotachophoresis / Wielders, J. P. M. / Everaerts, F. M. -- Thin-Layer Polyacrylamide Gel Isotachophoresis, Crossed Immuno-Isotachophoresis and Crossed Isotachophoresis-Electrofocusing / Brogren, C. H. -- Isotachophoresis of the Cerebrospinal Fluid (CSF) Proteins: Advantage of Prior Dialysis and its Practical Realization / Delmotte, P. -- 2. Applications -- Plasma Protein Fractionation in Sephadex by Isotachophoresis Using Discrete Spacers / Bier, M. / Kopwillem, A. -- Immuno-lsoelectric Focusing Analysis of Antibodies Fractionated by Isotachophoresis / Peltre, G. / Brogren, C. H. -- Analytical and Preparative Isotachophoresis in Column and Flat-Bed Gels for Fractionation of Antibodies / Brogren, C. H. / Peltre, G. -- Index -- Backmatter restricted access http://purl.org/coar/access_right/c_16ec online access with authorization star Mode of access: Internet via World Wide Web. In German. Description based on online resource; title from PDF title page (publisher's Web site, viewed 23. Jul 2019) SCIENCE / General. bisacsh Graesslin, D., editor. Radola, B. J., editor. print 9783110070262 https://doi.org/10.1515/9783110873870 Cover https://www.degruyter.com/cover/covers/9783110873870.jpg |
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Graesslin, D., Radola, B. J., |
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Graesslin, D., Radola, B. J., |
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Kolin, A. -- Rilbe, H. -- Chrambach, A. / Nguyen, Nga Y. -- Fawcett, J. S. -- Fredriksson, S. -- Brown, R. K. / Caspers, M. L. / Vinogradow, S. N. -- Radola, B. J. / Tschesche, H. / Schuricht, H. -- Grubhofer, N. / Borja, C. -- Vesterberg, O. / Hansén, L. -- Bispink, G. / Neuhoff, V. -- Zeineh, R. A. -- Leaback, D. H. -- Rosengren, Å. / Bjellqvist, B. / Gasparic, V. -- Gianazza, E. / Righetti, P. G. / Bordi, S. / Papeschi, G. -- Delincée, H. / Radola, B. J. -- Bengtsson, G. / Olivecrona, T. -- Righetti, P. G. / Gianazza, E. / Salamini, F. / Galante, E. / Viotti, A. / Soave, C. -- Rücker, W. / Markotai, J. -- Narayanan, K. R. / Ray, A. S. -- Kubicz, A. / Wolanska, L. -- Drysdale, J. W. -- Allen, R. C. / Oulla, P. M. / Arnaud, P. / Baumstark, J. S. -- Arnaud, P. / Chapuis-Cellier, C. / Wilson, G. B. / Koistinen, J. / Allen, R. C. / Fudenberg, H. H. -- Jeppsson, J. O. -- Utermann, G. / Hess, M. / Vogelberg, K. H. -- Altland, K. -- Bours, J. -- Das, P. K. / Graesslin, D. / Goedde, H. W. -- Hamann, A. -- Wilson, G. B. / Monsher, M. T. / Arnaud, P. / Fudenberg, H. H. -- Hamberg, U. / Turpeinen, U. / Knuutinen, U. -- Pronk, J. C. -- Ivarie, R. D. / Gelfand, D. H. / Jones, P. P. / O'Farrell, P. Z. / Polisky, B. A. / Steinberg, R. A. / O'Farrell, P. H. -- Stegemann, H. -- Kniifermann, H. -- Czempiel, W. -- Holmberg, K. / Wadström, T. -- Denckla, W. D. -- Winter, A. -- Wadstrom, T. / Möllby, R. / Olsson, B. / Söderholm, J. / Smyth, C. J. -- Quast, R. -- Sluyterman, L. A. A. E. / Wijdenes, J. -- Catsimpoolas, N. / Griffith, A. L. -- Just, W. W. / Werner, G. -- Manske, W. / Bohn, B. / Brossmer, R. -- Arlinger, L. -- Moberg, U. / Hjalmarsson, S. G. / Arlinger, L. / Lundin, H. -- Wielders, J. P. M. / Everaerts, F. M. -- Brogren, C. H. -- Delmotte, P. -- Bier, M. / Kopwillem, A. -- Peltre, G. / Brogren, C. H. -- Brogren, C. H. / Peltre, G. -- |
title |
Electrofocusing and Isotachophoresis : Proceedings of the International Symposium, August 1-4, 1976, Hamburg, Germany / |
spellingShingle |
Electrofocusing and Isotachophoresis : Proceedings of the International Symposium, August 1-4, 1976, Hamburg, Germany / Frontmatter -- Preface -- Contents -- Contributors -- Part I. Isoelectric Focusing -- 1. Theoretical and General Aspects -- Reminiscences about the Genesis of Isoelectric Focusing and Generalization of the Idea / Stable pH Gradients - A Key Problem in Isoelectric Focusing / Electrofocusing in Buffers: Formation of Natural pH Gradients, Flexibility, Gradient Stability, Relation to Isotachophoresis and Preparative Potential / Comparison of pH Gradients Used for Isoelectric Focusing / On the Temperature Dependence of Isoelectric Points of Proteins with Special Reference to Isoelectric Focusing / 2. Carrier Ampholytes -- Carrier Ampholyte Distribution / Characterization of Carrier Ampholytes by New Visualization Reactions and Chromatographic Methods / Synthesis of Carrier Ampholytes for Isoelectric Focusing Containing Sulfonic and Phosphonic Acid Groups Covering a Wide pH Range / 3. Analytical Methodology -- Staining of Proteins in Polyacrylamide Gels / Isoelectric Focusing of Complex Protein Mixtures in the Nanogram Range and Enzyme Kinetics of Dehydrogenases Following IEF / Soft Laser Scanning Densitometer Compatible with the High Resolution Obtained by Electrofocusing / Immunocore Electrofocusing: A Separation and Detection Technique Amenable to Scanning Densitometry / Rapid, Convenient and Economical Procedures for the Determination of the Isoelectric Spectra of Proteins / A Simple Method of Choosing Optimum pH-Conditions for Electrophoresis / pH Determinations in Isoelectric Focusing with an Iridium Electrode / Anomalous Behaviour of Horseradish Peroxidase in Isoelectric Focusing / Does Lipoprotein Lipase Bind Ampholytes? / 4. Biochemical Applications -- Zein: Macromolecular Properties, Biosynthesis and Genetic Regulation / Growth and Isoelectric Patterns of Peroxidase in Tobacco Tissue Cultures Under the Influence of Growth Regulator Systems / A Method for Simultaneous Analysis of Several Genetic Loci in Mice After Electrofocusing / Phylogenetic Differences in Isoelectric Components of Liver Acid Phosphatase and LDH Between Genera of Two Closely Related Rodent Families (Muridae and Microtidae) / Fractionation of Nucleic Acids on Isoelectric Focusing / 5. Clinical Applications -- Polyacrylamide Gel Isoelectric Focusing of Alpha-1-Proteinase Inhibitors (α1 Pi) Phenotypes: I. Optimal Condition, Histochemical and Proteinase Probe Studies / Polyacrylamide Gel Isoelectric Focusing (PAGIF) Applied to Alpha-1-Proteinase Inhibitor (α1 Pi) Phenotyping: II. Immunochemical Procedures / High Resolution Phenotyping of α1-Antitrypsins by Thin-Layer Polyacrylamide Electrofocusing / Broad-Beta Disease (Hyperlipoproteinaemia Type III): Genetics, Gene Frequency and Diagnosis without Ultracentrifugation / Screening for Abnormal Hemoglobins in the Newborn: A Highly Economic Procedure Using Isoelectric Focusing / The Crystallins of the Aging Lens from Five Species Studies by Various Methods of Thin-Layer Isoelectric Focusing / Separation of Erythrocyte Membrane Components in Electric Field. Polyacrylamide Gel Isoelectric Focusing: A Potential Method for the Diagnosis of Membrane Abnormality / Microheterogeneity of Serum Glycoproteins as Revealed by Flat-Bed Gel Isoelectric Focusing / Diagnosis of Cystic Fibrosis (CF) - Purification and Distinction Between CF Protein and Ciliary Dyskinesia Activity in CF and Asthmatic Sera Using Isoelectric Focusing and the Rabbit Tracheal Ciliary Bioassay / Microheterogeneity of Human Kininogen, Alpha- 2-HS and Alpha-1-Acid Glycoproteins by Thin- Layer Isoelectric Focusing / A Genetic Variant of Amylase from Human Parotid Saliva Detected by Isoelectric Focusing / 6. Two-Dimensional Techniques -- Biological Applications of Two-Dimensional Gel Electrophoresis / Plant Proteins Evaluated by Two-Dimensional Methods / Two-Dimensional Separation of Lymphocyte Microsomal Membrane Proteins: Isoelectric Focusing Linked to SDS-Polyacrylamide Gel Electrophoresis / Mammalian Mitochondrial Ribosomes: Two- Dimensional Gel Electrophoresis of the 55 S- and the Corresponding Subunit Proteins / Crossed Immunoelectrofocusing for Standardization and Characterization of Fungal Antigens / 7. Preparative Separations -- Isoelectric Focusing in Free Water / Preparative Electrofocusing in Flat-Beds of Granulated Gel - Methodological Aspects / Preparative Scale Purification of Bacterial Enzymes and Toxins by Isoelectric Focusing and Isotachophoresis / Preparative Zone Convection Electrofocusing in Helical Glass Tubes / Chromatofocusing: Isoelectric Focusing on Ion Exchangers in the Absence of an Externally Applied Potential / 8. Separation of Cells -- New Instrumentation and Procedures for the Analysis of Mammalian Cells by Electrophoretic Techniques / Continuous-Flow Isoelectric Focusing: Further Studies on Human Red Blood Cells and on the Separation of Rat Liver Light Mitochondrial Fraction / Preparative Electrofocusing of Native and Modified Living Mammalian Cells in a Stationary Ficoll/ Sucrose Gradient / Part II. Isotachophoresis -- 1. Analytical and Preparative Methodology -- The Principle of Preparative Capillary Isotachophoresis / Preparative Capillary Isotachophoresis: Separation of μg Amounts of Some Human Serum Proteins / Quantitative Determination of Picomoles ADP by Means of Steady State Mixed Zones in Isotachophoresis / Thin-Layer Polyacrylamide Gel Isotachophoresis, Crossed Immuno-Isotachophoresis and Crossed Isotachophoresis-Electrofocusing / Isotachophoresis of the Cerebrospinal Fluid (CSF) Proteins: Advantage of Prior Dialysis and its Practical Realization / 2. Applications -- Plasma Protein Fractionation in Sephadex by Isotachophoresis Using Discrete Spacers / Immuno-lsoelectric Focusing Analysis of Antibodies Fractionated by Isotachophoresis / Analytical and Preparative Isotachophoresis in Column and Flat-Bed Gels for Fractionation of Antibodies / Index -- Backmatter |
title_sub |
Proceedings of the International Symposium, August 1-4, 1976, Hamburg, Germany / |
title_full |
Electrofocusing and Isotachophoresis : Proceedings of the International Symposium, August 1-4, 1976, Hamburg, Germany / B. J. Radola, D. Graesslin. |
title_fullStr |
Electrofocusing and Isotachophoresis : Proceedings of the International Symposium, August 1-4, 1976, Hamburg, Germany / B. J. Radola, D. Graesslin. |
title_full_unstemmed |
Electrofocusing and Isotachophoresis : Proceedings of the International Symposium, August 1-4, 1976, Hamburg, Germany / B. J. Radola, D. Graesslin. |
title_auth |
Electrofocusing and Isotachophoresis : Proceedings of the International Symposium, August 1-4, 1976, Hamburg, Germany / |
title_alt |
Frontmatter -- Preface -- Contents -- Contributors -- Part I. Isoelectric Focusing -- 1. Theoretical and General Aspects -- Reminiscences about the Genesis of Isoelectric Focusing and Generalization of the Idea / Stable pH Gradients - A Key Problem in Isoelectric Focusing / Electrofocusing in Buffers: Formation of Natural pH Gradients, Flexibility, Gradient Stability, Relation to Isotachophoresis and Preparative Potential / Comparison of pH Gradients Used for Isoelectric Focusing / On the Temperature Dependence of Isoelectric Points of Proteins with Special Reference to Isoelectric Focusing / 2. Carrier Ampholytes -- Carrier Ampholyte Distribution / Characterization of Carrier Ampholytes by New Visualization Reactions and Chromatographic Methods / Synthesis of Carrier Ampholytes for Isoelectric Focusing Containing Sulfonic and Phosphonic Acid Groups Covering a Wide pH Range / 3. Analytical Methodology -- Staining of Proteins in Polyacrylamide Gels / Isoelectric Focusing of Complex Protein Mixtures in the Nanogram Range and Enzyme Kinetics of Dehydrogenases Following IEF / Soft Laser Scanning Densitometer Compatible with the High Resolution Obtained by Electrofocusing / Immunocore Electrofocusing: A Separation and Detection Technique Amenable to Scanning Densitometry / Rapid, Convenient and Economical Procedures for the Determination of the Isoelectric Spectra of Proteins / A Simple Method of Choosing Optimum pH-Conditions for Electrophoresis / pH Determinations in Isoelectric Focusing with an Iridium Electrode / Anomalous Behaviour of Horseradish Peroxidase in Isoelectric Focusing / Does Lipoprotein Lipase Bind Ampholytes? / 4. Biochemical Applications -- Zein: Macromolecular Properties, Biosynthesis and Genetic Regulation / Growth and Isoelectric Patterns of Peroxidase in Tobacco Tissue Cultures Under the Influence of Growth Regulator Systems / A Method for Simultaneous Analysis of Several Genetic Loci in Mice After Electrofocusing / Phylogenetic Differences in Isoelectric Components of Liver Acid Phosphatase and LDH Between Genera of Two Closely Related Rodent Families (Muridae and Microtidae) / Fractionation of Nucleic Acids on Isoelectric Focusing / 5. Clinical Applications -- Polyacrylamide Gel Isoelectric Focusing of Alpha-1-Proteinase Inhibitors (α1 Pi) Phenotypes: I. Optimal Condition, Histochemical and Proteinase Probe Studies / Polyacrylamide Gel Isoelectric Focusing (PAGIF) Applied to Alpha-1-Proteinase Inhibitor (α1 Pi) Phenotyping: II. Immunochemical Procedures / High Resolution Phenotyping of α1-Antitrypsins by Thin-Layer Polyacrylamide Electrofocusing / Broad-Beta Disease (Hyperlipoproteinaemia Type III): Genetics, Gene Frequency and Diagnosis without Ultracentrifugation / Screening for Abnormal Hemoglobins in the Newborn: A Highly Economic Procedure Using Isoelectric Focusing / The Crystallins of the Aging Lens from Five Species Studies by Various Methods of Thin-Layer Isoelectric Focusing / Separation of Erythrocyte Membrane Components in Electric Field. Polyacrylamide Gel Isoelectric Focusing: A Potential Method for the Diagnosis of Membrane Abnormality / Microheterogeneity of Serum Glycoproteins as Revealed by Flat-Bed Gel Isoelectric Focusing / Diagnosis of Cystic Fibrosis (CF) - Purification and Distinction Between CF Protein and Ciliary Dyskinesia Activity in CF and Asthmatic Sera Using Isoelectric Focusing and the Rabbit Tracheal Ciliary Bioassay / Microheterogeneity of Human Kininogen, Alpha- 2-HS and Alpha-1-Acid Glycoproteins by Thin- Layer Isoelectric Focusing / A Genetic Variant of Amylase from Human Parotid Saliva Detected by Isoelectric Focusing / 6. Two-Dimensional Techniques -- Biological Applications of Two-Dimensional Gel Electrophoresis / Plant Proteins Evaluated by Two-Dimensional Methods / Two-Dimensional Separation of Lymphocyte Microsomal Membrane Proteins: Isoelectric Focusing Linked to SDS-Polyacrylamide Gel Electrophoresis / Mammalian Mitochondrial Ribosomes: Two- Dimensional Gel Electrophoresis of the 55 S- and the Corresponding Subunit Proteins / Crossed Immunoelectrofocusing for Standardization and Characterization of Fungal Antigens / 7. Preparative Separations -- Isoelectric Focusing in Free Water / Preparative Electrofocusing in Flat-Beds of Granulated Gel - Methodological Aspects / Preparative Scale Purification of Bacterial Enzymes and Toxins by Isoelectric Focusing and Isotachophoresis / Preparative Zone Convection Electrofocusing in Helical Glass Tubes / Chromatofocusing: Isoelectric Focusing on Ion Exchangers in the Absence of an Externally Applied Potential / 8. Separation of Cells -- New Instrumentation and Procedures for the Analysis of Mammalian Cells by Electrophoretic Techniques / Continuous-Flow Isoelectric Focusing: Further Studies on Human Red Blood Cells and on the Separation of Rat Liver Light Mitochondrial Fraction / Preparative Electrofocusing of Native and Modified Living Mammalian Cells in a Stationary Ficoll/ Sucrose Gradient / Part II. Isotachophoresis -- 1. Analytical and Preparative Methodology -- The Principle of Preparative Capillary Isotachophoresis / Preparative Capillary Isotachophoresis: Separation of μg Amounts of Some Human Serum Proteins / Quantitative Determination of Picomoles ADP by Means of Steady State Mixed Zones in Isotachophoresis / Thin-Layer Polyacrylamide Gel Isotachophoresis, Crossed Immuno-Isotachophoresis and Crossed Isotachophoresis-Electrofocusing / Isotachophoresis of the Cerebrospinal Fluid (CSF) Proteins: Advantage of Prior Dialysis and its Practical Realization / 2. Applications -- Plasma Protein Fractionation in Sephadex by Isotachophoresis Using Discrete Spacers / Immuno-lsoelectric Focusing Analysis of Antibodies Fractionated by Isotachophoresis / Analytical and Preparative Isotachophoresis in Column and Flat-Bed Gels for Fractionation of Antibodies / Index -- Backmatter |
title_new |
Electrofocusing and Isotachophoresis : |
title_sort |
electrofocusing and isotachophoresis : proceedings of the international symposium, august 1-4, 1976, hamburg, germany / |
publisher |
De Gruyter, |
publishDate |
2019 |
physical |
1 online resource (624 p.) |
edition |
Reprint 2019 |
contents |
Frontmatter -- Preface -- Contents -- Contributors -- Part I. Isoelectric Focusing -- 1. Theoretical and General Aspects -- Reminiscences about the Genesis of Isoelectric Focusing and Generalization of the Idea / Stable pH Gradients - A Key Problem in Isoelectric Focusing / Electrofocusing in Buffers: Formation of Natural pH Gradients, Flexibility, Gradient Stability, Relation to Isotachophoresis and Preparative Potential / Comparison of pH Gradients Used for Isoelectric Focusing / On the Temperature Dependence of Isoelectric Points of Proteins with Special Reference to Isoelectric Focusing / 2. Carrier Ampholytes -- Carrier Ampholyte Distribution / Characterization of Carrier Ampholytes by New Visualization Reactions and Chromatographic Methods / Synthesis of Carrier Ampholytes for Isoelectric Focusing Containing Sulfonic and Phosphonic Acid Groups Covering a Wide pH Range / 3. Analytical Methodology -- Staining of Proteins in Polyacrylamide Gels / Isoelectric Focusing of Complex Protein Mixtures in the Nanogram Range and Enzyme Kinetics of Dehydrogenases Following IEF / Soft Laser Scanning Densitometer Compatible with the High Resolution Obtained by Electrofocusing / Immunocore Electrofocusing: A Separation and Detection Technique Amenable to Scanning Densitometry / Rapid, Convenient and Economical Procedures for the Determination of the Isoelectric Spectra of Proteins / A Simple Method of Choosing Optimum pH-Conditions for Electrophoresis / pH Determinations in Isoelectric Focusing with an Iridium Electrode / Anomalous Behaviour of Horseradish Peroxidase in Isoelectric Focusing / Does Lipoprotein Lipase Bind Ampholytes? / 4. Biochemical Applications -- Zein: Macromolecular Properties, Biosynthesis and Genetic Regulation / Growth and Isoelectric Patterns of Peroxidase in Tobacco Tissue Cultures Under the Influence of Growth Regulator Systems / A Method for Simultaneous Analysis of Several Genetic Loci in Mice After Electrofocusing / Phylogenetic Differences in Isoelectric Components of Liver Acid Phosphatase and LDH Between Genera of Two Closely Related Rodent Families (Muridae and Microtidae) / Fractionation of Nucleic Acids on Isoelectric Focusing / 5. Clinical Applications -- Polyacrylamide Gel Isoelectric Focusing of Alpha-1-Proteinase Inhibitors (α1 Pi) Phenotypes: I. Optimal Condition, Histochemical and Proteinase Probe Studies / Polyacrylamide Gel Isoelectric Focusing (PAGIF) Applied to Alpha-1-Proteinase Inhibitor (α1 Pi) Phenotyping: II. Immunochemical Procedures / High Resolution Phenotyping of α1-Antitrypsins by Thin-Layer Polyacrylamide Electrofocusing / Broad-Beta Disease (Hyperlipoproteinaemia Type III): Genetics, Gene Frequency and Diagnosis without Ultracentrifugation / Screening for Abnormal Hemoglobins in the Newborn: A Highly Economic Procedure Using Isoelectric Focusing / The Crystallins of the Aging Lens from Five Species Studies by Various Methods of Thin-Layer Isoelectric Focusing / Separation of Erythrocyte Membrane Components in Electric Field. Polyacrylamide Gel Isoelectric Focusing: A Potential Method for the Diagnosis of Membrane Abnormality / Microheterogeneity of Serum Glycoproteins as Revealed by Flat-Bed Gel Isoelectric Focusing / Diagnosis of Cystic Fibrosis (CF) - Purification and Distinction Between CF Protein and Ciliary Dyskinesia Activity in CF and Asthmatic Sera Using Isoelectric Focusing and the Rabbit Tracheal Ciliary Bioassay / Microheterogeneity of Human Kininogen, Alpha- 2-HS and Alpha-1-Acid Glycoproteins by Thin- Layer Isoelectric Focusing / A Genetic Variant of Amylase from Human Parotid Saliva Detected by Isoelectric Focusing / 6. Two-Dimensional Techniques -- Biological Applications of Two-Dimensional Gel Electrophoresis / Plant Proteins Evaluated by Two-Dimensional Methods / Two-Dimensional Separation of Lymphocyte Microsomal Membrane Proteins: Isoelectric Focusing Linked to SDS-Polyacrylamide Gel Electrophoresis / Mammalian Mitochondrial Ribosomes: Two- Dimensional Gel Electrophoresis of the 55 S- and the Corresponding Subunit Proteins / Crossed Immunoelectrofocusing for Standardization and Characterization of Fungal Antigens / 7. Preparative Separations -- Isoelectric Focusing in Free Water / Preparative Electrofocusing in Flat-Beds of Granulated Gel - Methodological Aspects / Preparative Scale Purification of Bacterial Enzymes and Toxins by Isoelectric Focusing and Isotachophoresis / Preparative Zone Convection Electrofocusing in Helical Glass Tubes / Chromatofocusing: Isoelectric Focusing on Ion Exchangers in the Absence of an Externally Applied Potential / 8. Separation of Cells -- New Instrumentation and Procedures for the Analysis of Mammalian Cells by Electrophoretic Techniques / Continuous-Flow Isoelectric Focusing: Further Studies on Human Red Blood Cells and on the Separation of Rat Liver Light Mitochondrial Fraction / Preparative Electrofocusing of Native and Modified Living Mammalian Cells in a Stationary Ficoll/ Sucrose Gradient / Part II. Isotachophoresis -- 1. Analytical and Preparative Methodology -- The Principle of Preparative Capillary Isotachophoresis / Preparative Capillary Isotachophoresis: Separation of μg Amounts of Some Human Serum Proteins / Quantitative Determination of Picomoles ADP by Means of Steady State Mixed Zones in Isotachophoresis / Thin-Layer Polyacrylamide Gel Isotachophoresis, Crossed Immuno-Isotachophoresis and Crossed Isotachophoresis-Electrofocusing / Isotachophoresis of the Cerebrospinal Fluid (CSF) Proteins: Advantage of Prior Dialysis and its Practical Realization / 2. Applications -- Plasma Protein Fractionation in Sephadex by Isotachophoresis Using Discrete Spacers / Immuno-lsoelectric Focusing Analysis of Antibodies Fractionated by Isotachophoresis / Analytical and Preparative Isotachophoresis in Column and Flat-Bed Gels for Fractionation of Antibodies / Index -- Backmatter |
isbn |
9783110873870 9783110070262 |
url |
https://doi.org/10.1515/9783110873870 https://www.degruyter.com/cover/covers/9783110873870.jpg |
illustrated |
Not Illustrated |
doi_str_mv |
10.1515/9783110873870 |
oclc_num |
1110712791 |
work_keys_str_mv |
AT graesslind electrofocusingandisotachophoresisproceedingsoftheinternationalsymposiumaugust141976hamburggermany AT radolabj electrofocusingandisotachophoresisproceedingsoftheinternationalsymposiumaugust141976hamburggermany |
status_str |
n |
ids_txt_mv |
(DE-B1597)39933 (OCoLC)1110712791 |
carrierType_str_mv |
cr |
is_hierarchy_title |
Electrofocusing and Isotachophoresis : Proceedings of the International Symposium, August 1-4, 1976, Hamburg, Germany / |
author2_original_writing_str_mv |
noLinkedField noLinkedField |
_version_ |
1770177981753327616 |
fullrecord |
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J. Radola, D. Graesslin.</subfield></datafield><datafield tag="250" ind1=" " ind2=" "><subfield code="a">Reprint 2019</subfield></datafield><datafield tag="264" ind1=" " ind2="1"><subfield code="a">Berlin ;</subfield><subfield code="a">Boston : </subfield><subfield code="b">De Gruyter, </subfield><subfield code="c">[2019]</subfield></datafield><datafield tag="264" ind1=" " ind2="4"><subfield code="c">©1977</subfield></datafield><datafield tag="300" ind1=" " ind2=" "><subfield code="a">1 online resource (624 p.)</subfield></datafield><datafield tag="336" ind1=" " ind2=" "><subfield code="a">text</subfield><subfield code="b">txt</subfield><subfield code="2">rdacontent</subfield></datafield><datafield tag="337" ind1=" " ind2=" "><subfield code="a">computer</subfield><subfield code="b">c</subfield><subfield code="2">rdamedia</subfield></datafield><datafield tag="338" ind1=" " ind2=" "><subfield code="a">online resource</subfield><subfield code="b">cr</subfield><subfield code="2">rdacarrier</subfield></datafield><datafield tag="347" ind1=" " ind2=" "><subfield code="a">text file</subfield><subfield code="b">PDF</subfield><subfield code="2">rda</subfield></datafield><datafield tag="505" ind1="0" ind2="0"><subfield code="t">Frontmatter -- </subfield><subfield code="t">Preface -- </subfield><subfield code="t">Contents -- </subfield><subfield code="t">Contributors -- </subfield><subfield code="t">Part I. Isoelectric Focusing -- </subfield><subfield code="t">1. Theoretical and General Aspects -- </subfield><subfield code="t">Reminiscences about the Genesis of Isoelectric Focusing and Generalization of the Idea / </subfield><subfield code="r">Kolin, A. -- </subfield><subfield code="t">Stable pH Gradients - A Key Problem in Isoelectric Focusing / </subfield><subfield code="r">Rilbe, H. -- </subfield><subfield code="t">Electrofocusing in Buffers: Formation of Natural pH Gradients, Flexibility, Gradient Stability, Relation to Isotachophoresis and Preparative Potential / </subfield><subfield code="r">Chrambach, A. / Nguyen, Nga Y. -- </subfield><subfield code="t">Comparison of pH Gradients Used for Isoelectric Focusing / </subfield><subfield code="r">Fawcett, J. S. -- </subfield><subfield code="t">On the Temperature Dependence of Isoelectric Points of Proteins with Special Reference to Isoelectric Focusing / </subfield><subfield code="r">Fredriksson, S. -- </subfield><subfield code="t">2. Carrier Ampholytes -- </subfield><subfield code="t">Carrier Ampholyte Distribution / </subfield><subfield code="r">Brown, R. K. / Caspers, M. L. / Vinogradow, S. N. -- </subfield><subfield code="t">Characterization of Carrier Ampholytes by New Visualization Reactions and Chromatographic Methods / </subfield><subfield code="r">Radola, B. J. / Tschesche, H. / Schuricht, H. -- </subfield><subfield code="t">Synthesis of Carrier Ampholytes for Isoelectric Focusing Containing Sulfonic and Phosphonic Acid Groups Covering a Wide pH Range / </subfield><subfield code="r">Grubhofer, N. / Borja, C. -- </subfield><subfield code="t">3. Analytical Methodology -- </subfield><subfield code="t">Staining of Proteins in Polyacrylamide Gels / </subfield><subfield code="r">Vesterberg, O. / Hansén, L. -- </subfield><subfield code="t">Isoelectric Focusing of Complex Protein Mixtures in the Nanogram Range and Enzyme Kinetics of Dehydrogenases Following IEF / </subfield><subfield code="r">Bispink, G. / Neuhoff, V. -- </subfield><subfield code="t">Soft Laser Scanning Densitometer Compatible with the High Resolution Obtained by Electrofocusing / </subfield><subfield code="r">Zeineh, R. A. -- </subfield><subfield code="t">Immunocore Electrofocusing: A Separation and Detection Technique Amenable to Scanning Densitometry / </subfield><subfield code="r">Zeineh, R. A. -- </subfield><subfield code="t">Rapid, Convenient and Economical Procedures for the Determination of the Isoelectric Spectra of Proteins / </subfield><subfield code="r">Leaback, D. H. -- </subfield><subfield code="t">A Simple Method of Choosing Optimum pH-Conditions for Electrophoresis / </subfield><subfield code="r">Rosengren, Å. / Bjellqvist, B. / Gasparic, V. -- </subfield><subfield code="t">pH Determinations in Isoelectric Focusing with an Iridium Electrode / </subfield><subfield code="r">Gianazza, E. / Righetti, P. G. / Bordi, S. / Papeschi, G. -- </subfield><subfield code="t">Anomalous Behaviour of Horseradish Peroxidase in Isoelectric Focusing / </subfield><subfield code="r">Delincée, H. / Radola, B. J. -- </subfield><subfield code="t">Does Lipoprotein Lipase Bind Ampholytes? / </subfield><subfield code="r">Bengtsson, G. / Olivecrona, T. -- </subfield><subfield code="t">4. Biochemical Applications -- </subfield><subfield code="t">Zein: Macromolecular Properties, Biosynthesis and Genetic Regulation / </subfield><subfield code="r">Righetti, P. G. / Gianazza, E. / Salamini, F. / Galante, E. / Viotti, A. / Soave, C. -- </subfield><subfield code="t">Growth and Isoelectric Patterns of Peroxidase in Tobacco Tissue Cultures Under the Influence of Growth Regulator Systems / </subfield><subfield code="r">Rücker, W. / Markotai, J. -- </subfield><subfield code="t">A Method for Simultaneous Analysis of Several Genetic Loci in Mice After Electrofocusing / </subfield><subfield code="r">Narayanan, K. R. / Ray, A. S. -- </subfield><subfield code="t">Phylogenetic Differences in Isoelectric Components of Liver Acid Phosphatase and LDH Between Genera of Two Closely Related Rodent Families (Muridae and Microtidae) / </subfield><subfield code="r">Kubicz, A. / Wolanska, L. -- </subfield><subfield code="t">Fractionation of Nucleic Acids on Isoelectric Focusing / </subfield><subfield code="r">Drysdale, J. W. -- </subfield><subfield code="t">5. Clinical Applications -- </subfield><subfield code="t">Polyacrylamide Gel Isoelectric Focusing of Alpha-1-Proteinase Inhibitors (α1 Pi) Phenotypes: I. Optimal Condition, Histochemical and Proteinase Probe Studies / </subfield><subfield code="r">Allen, R. C. / Oulla, P. M. / Arnaud, P. / Baumstark, J. S. -- </subfield><subfield code="t">Polyacrylamide Gel Isoelectric Focusing (PAGIF) Applied to Alpha-1-Proteinase Inhibitor (α1 Pi) Phenotyping: II. Immunochemical Procedures / </subfield><subfield code="r">Arnaud, P. / Chapuis-Cellier, C. / Wilson, G. B. / Koistinen, J. / Allen, R. C. / Fudenberg, H. H. -- </subfield><subfield code="t">High Resolution Phenotyping of α1-Antitrypsins by Thin-Layer Polyacrylamide Electrofocusing / </subfield><subfield code="r">Jeppsson, J. O. -- </subfield><subfield code="t">Broad-Beta Disease (Hyperlipoproteinaemia Type III): Genetics, Gene Frequency and Diagnosis without Ultracentrifugation / </subfield><subfield code="r">Utermann, G. / Hess, M. / Vogelberg, K. H. -- </subfield><subfield code="t">Screening for Abnormal Hemoglobins in the Newborn: A Highly Economic Procedure Using Isoelectric Focusing / </subfield><subfield code="r">Altland, K. -- </subfield><subfield code="t">The Crystallins of the Aging Lens from Five Species Studies by Various Methods of Thin-Layer Isoelectric Focusing / </subfield><subfield code="r">Bours, J. -- </subfield><subfield code="t">Separation of Erythrocyte Membrane Components in Electric Field. Polyacrylamide Gel Isoelectric Focusing: A Potential Method for the Diagnosis of Membrane Abnormality / </subfield><subfield code="r">Das, P. K. / Graesslin, D. / Goedde, H. W. -- </subfield><subfield code="t">Microheterogeneity of Serum Glycoproteins as Revealed by Flat-Bed Gel Isoelectric Focusing / </subfield><subfield code="r">Hamann, A. -- </subfield><subfield code="t">Diagnosis of Cystic Fibrosis (CF) - Purification and Distinction Between CF Protein and Ciliary Dyskinesia Activity in CF and Asthmatic Sera Using Isoelectric Focusing and the Rabbit Tracheal Ciliary Bioassay / </subfield><subfield code="r">Wilson, G. B. / Monsher, M. T. / Arnaud, P. / Fudenberg, H. H. -- </subfield><subfield code="t">Microheterogeneity of Human Kininogen, Alpha- 2-HS and Alpha-1-Acid Glycoproteins by Thin- Layer Isoelectric Focusing / </subfield><subfield code="r">Hamberg, U. / Turpeinen, U. / Knuutinen, U. -- </subfield><subfield code="t">A Genetic Variant of Amylase from Human Parotid Saliva Detected by Isoelectric Focusing / </subfield><subfield code="r">Pronk, J. C. -- </subfield><subfield code="t">6. Two-Dimensional Techniques -- </subfield><subfield code="t">Biological Applications of Two-Dimensional Gel Electrophoresis / </subfield><subfield code="r">Ivarie, R. D. / Gelfand, D. H. / Jones, P. P. / O'Farrell, P. Z. / Polisky, B. A. / Steinberg, R. A. / O'Farrell, P. H. -- </subfield><subfield code="t">Plant Proteins Evaluated by Two-Dimensional Methods / </subfield><subfield code="r">Stegemann, H. -- </subfield><subfield code="t">Two-Dimensional Separation of Lymphocyte Microsomal Membrane Proteins: Isoelectric Focusing Linked to SDS-Polyacrylamide Gel Electrophoresis / </subfield><subfield code="r">Kniifermann, H. -- </subfield><subfield code="t">Mammalian Mitochondrial Ribosomes: Two- Dimensional Gel Electrophoresis of the 55 S- and the Corresponding Subunit Proteins / </subfield><subfield code="r">Czempiel, W. -- </subfield><subfield code="t">Crossed Immunoelectrofocusing for Standardization and Characterization of Fungal Antigens / </subfield><subfield code="r">Holmberg, K. / Wadström, T. -- </subfield><subfield code="t">7. Preparative Separations -- </subfield><subfield code="t">Isoelectric Focusing in Free Water / </subfield><subfield code="r">Denckla, W. D. -- </subfield><subfield code="t">Preparative Electrofocusing in Flat-Beds of Granulated Gel - Methodological Aspects / </subfield><subfield code="r">Winter, A. -- </subfield><subfield code="t">Preparative Scale Purification of Bacterial Enzymes and Toxins by Isoelectric Focusing and Isotachophoresis / </subfield><subfield code="r">Wadstrom, T. / Möllby, R. / Olsson, B. / Söderholm, J. / Smyth, C. J. -- </subfield><subfield code="t">Preparative Zone Convection Electrofocusing in Helical Glass Tubes / </subfield><subfield code="r">Quast, R. -- </subfield><subfield code="t">Chromatofocusing: Isoelectric Focusing on Ion Exchangers in the Absence of an Externally Applied Potential / </subfield><subfield code="r">Sluyterman, L. A. A. E. / Wijdenes, J. -- </subfield><subfield code="t">8. Separation of Cells -- </subfield><subfield code="t">New Instrumentation and Procedures for the Analysis of Mammalian Cells by Electrophoretic Techniques / </subfield><subfield code="r">Catsimpoolas, N. / Griffith, A. L. -- </subfield><subfield code="t">Continuous-Flow Isoelectric Focusing: Further Studies on Human Red Blood Cells and on the Separation of Rat Liver Light Mitochondrial Fraction / </subfield><subfield code="r">Just, W. W. / Werner, G. -- </subfield><subfield code="t">Preparative Electrofocusing of Native and Modified Living Mammalian Cells in a Stationary Ficoll/ Sucrose Gradient / </subfield><subfield code="r">Manske, W. / Bohn, B. / Brossmer, R. -- </subfield><subfield code="t">Part II. Isotachophoresis -- </subfield><subfield code="t">1. Analytical and Preparative Methodology -- </subfield><subfield code="t">The Principle of Preparative Capillary Isotachophoresis / </subfield><subfield code="r">Arlinger, L. -- </subfield><subfield code="t">Preparative Capillary Isotachophoresis: Separation of μg Amounts of Some Human Serum Proteins / </subfield><subfield code="r">Moberg, U. / Hjalmarsson, S. G. / Arlinger, L. / Lundin, H. -- </subfield><subfield code="t">Quantitative Determination of Picomoles ADP by Means of Steady State Mixed Zones in Isotachophoresis / </subfield><subfield code="r">Wielders, J. P. M. / Everaerts, F. M. -- </subfield><subfield code="t">Thin-Layer Polyacrylamide Gel Isotachophoresis, Crossed Immuno-Isotachophoresis and Crossed Isotachophoresis-Electrofocusing / </subfield><subfield code="r">Brogren, C. H. -- </subfield><subfield code="t">Isotachophoresis of the Cerebrospinal Fluid (CSF) Proteins: Advantage of Prior Dialysis and its Practical Realization / </subfield><subfield code="r">Delmotte, P. -- </subfield><subfield code="t">2. Applications -- </subfield><subfield code="t">Plasma Protein Fractionation in Sephadex by Isotachophoresis Using Discrete Spacers / </subfield><subfield code="r">Bier, M. / Kopwillem, A. -- </subfield><subfield code="t">Immuno-lsoelectric Focusing Analysis of Antibodies Fractionated by Isotachophoresis / </subfield><subfield code="r">Peltre, G. / Brogren, C. H. -- </subfield><subfield code="t">Analytical and Preparative Isotachophoresis in Column and Flat-Bed Gels for Fractionation of Antibodies / </subfield><subfield code="r">Brogren, C. H. / Peltre, G. -- </subfield><subfield code="t">Index -- </subfield><subfield code="t">Backmatter</subfield></datafield><datafield tag="506" ind1="0" ind2=" "><subfield code="a">restricted access</subfield><subfield code="u">http://purl.org/coar/access_right/c_16ec</subfield><subfield code="f">online access with authorization</subfield><subfield code="2">star</subfield></datafield><datafield tag="538" ind1=" " ind2=" "><subfield code="a">Mode of access: Internet via World Wide Web.</subfield></datafield><datafield tag="546" ind1=" " ind2=" "><subfield code="a">In German.</subfield></datafield><datafield tag="588" ind1="0" ind2=" "><subfield code="a">Description based on online resource; title from PDF title page (publisher's Web site, viewed 23. Jul 2019)</subfield></datafield><datafield tag="650" ind1=" " ind2="7"><subfield code="a">SCIENCE / General.</subfield><subfield code="2">bisacsh</subfield></datafield><datafield tag="700" ind1="1" ind2=" "><subfield code="a">Graesslin, D., </subfield><subfield code="e">editor.</subfield></datafield><datafield tag="700" ind1="1" ind2=" "><subfield code="a">Radola, B. 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