Electrofocusing and Isotachophoresis : : Proceedings of the International Symposium, August 1-4, 1976, Hamburg, Germany / / B. J. Radola, D. Graesslin.

• TeilnehmendeR: Graesslin, D.,; Radola, B. J.,
• Place / Publishing House: Berlin ;, Boston : : De Gruyter, , [2019]; ©1977
• Year of Publication: 2019
• Edition: Reprint 2019
• Language: German
• Physical Description: 1 online resource (624 p.)
• Other title: Frontmatter --; Preface --; Contents --; Contributors --; Part I. Isoelectric Focusing --; 1. Theoretical and General Aspects --; Reminiscences about the Genesis of Isoelectric Focusing and Generalization of the Idea /; Stable pH Gradients - A Key Problem in Isoelectric Focusing /; Electrofocusing in Buffers: Formation of Natural pH Gradients, Flexibility, Gradient Stability, Relation to Isotachophoresis and Preparative Potential /; Comparison of pH Gradients Used for Isoelectric Focusing /; On the Temperature Dependence of Isoelectric Points of Proteins with Special Reference to Isoelectric Focusing /; 2. Carrier Ampholytes --; Carrier Ampholyte Distribution /; Characterization of Carrier Ampholytes by New Visualization Reactions and Chromatographic Methods /; Synthesis of Carrier Ampholytes for Isoelectric Focusing Containing Sulfonic and Phosphonic Acid Groups Covering a Wide pH Range /; 3. Analytical Methodology --; Staining of Proteins in Polyacrylamide Gels /; Isoelectric Focusing of Complex Protein Mixtures in the Nanogram Range and Enzyme Kinetics of Dehydrogenases Following IEF /; Soft Laser Scanning Densitometer Compatible with the High Resolution Obtained by Electrofocusing /; Immunocore Electrofocusing: A Separation and Detection Technique Amenable to Scanning Densitometry /; Rapid, Convenient and Economical Procedures for the Determination of the Isoelectric Spectra of Proteins /; A Simple Method of Choosing Optimum pH-Conditions for Electrophoresis /; pH Determinations in Isoelectric Focusing with an Iridium Electrode /; Anomalous Behaviour of Horseradish Peroxidase in Isoelectric Focusing /; Does Lipoprotein Lipase Bind Ampholytes? /; 4. Biochemical Applications --; Zein: Macromolecular Properties, Biosynthesis and Genetic Regulation /; Growth and Isoelectric Patterns of Peroxidase in Tobacco Tissue Cultures Under the Influence of Growth Regulator Systems /; A Method for Simultaneous Analysis of Several Genetic Loci in Mice After Electrofocusing /; Phylogenetic Differences in Isoelectric Components of Liver Acid Phosphatase and LDH Between Genera of Two Closely Related Rodent Families (Muridae and Microtidae) /; Fractionation of Nucleic Acids on Isoelectric Focusing /; 5. Clinical Applications --; Polyacrylamide Gel Isoelectric Focusing of Alpha-1-Proteinase Inhibitors (α1 Pi) Phenotypes: I. Optimal Condition, Histochemical and Proteinase Probe Studies /; Polyacrylamide Gel Isoelectric Focusing (PAGIF) Applied to Alpha-1-Proteinase Inhibitor (α1 Pi) Phenotyping: II. Immunochemical Procedures /; High Resolution Phenotyping of α1-Antitrypsins by Thin-Layer Polyacrylamide Electrofocusing /; Broad-Beta Disease (Hyperlipoproteinaemia Type III): Genetics, Gene Frequency and Diagnosis without Ultracentrifugation /; Screening for Abnormal Hemoglobins in the Newborn: A Highly Economic Procedure Using Isoelectric Focusing /; The Crystallins of the Aging Lens from Five Species Studies by Various Methods of Thin-Layer Isoelectric Focusing /; Separation of Erythrocyte Membrane Components in Electric Field. Polyacrylamide Gel Isoelectric Focusing: A Potential Method for the Diagnosis of Membrane Abnormality /; Microheterogeneity of Serum Glycoproteins as Revealed by Flat-Bed Gel Isoelectric Focusing /; Diagnosis of Cystic Fibrosis (CF) - Purification and Distinction Between CF Protein and Ciliary Dyskinesia Activity in CF and Asthmatic Sera Using Isoelectric Focusing and the Rabbit Tracheal Ciliary Bioassay /; Microheterogeneity of Human Kininogen, Alpha- 2-HS and Alpha-1-Acid Glycoproteins by Thin- Layer Isoelectric Focusing /; A Genetic Variant of Amylase from Human Parotid Saliva Detected by Isoelectric Focusing /; 6. Two-Dimensional Techniques --; Biological Applications of Two-Dimensional Gel Electrophoresis /; Plant Proteins Evaluated by Two-Dimensional Methods /; Two-Dimensional Separation of Lymphocyte Microsomal Membrane Proteins: Isoelectric Focusing Linked to SDS-Polyacrylamide Gel Electrophoresis /; Mammalian Mitochondrial Ribosomes: Two- Dimensional Gel Electrophoresis of the 55 S- and the Corresponding Subunit Proteins /; Crossed Immunoelectrofocusing for Standardization and Characterization of Fungal Antigens /; 7. Preparative Separations --; Isoelectric Focusing in Free Water /; Preparative Electrofocusing in Flat-Beds of Granulated Gel - Methodological Aspects /; Preparative Scale Purification of Bacterial Enzymes and Toxins by Isoelectric Focusing and Isotachophoresis /; Preparative Zone Convection Electrofocusing in Helical Glass Tubes /; Chromatofocusing: Isoelectric Focusing on Ion Exchangers in the Absence of an Externally Applied Potential /; 8. Separation of Cells --; New Instrumentation and Procedures for the Analysis of Mammalian Cells by Electrophoretic Techniques /; Continuous-Flow Isoelectric Focusing: Further Studies on Human Red Blood Cells and on the Separation of Rat Liver Light Mitochondrial Fraction /; Preparative Electrofocusing of Native and Modified Living Mammalian Cells in a Stationary Ficoll/ Sucrose Gradient /; Part II. Isotachophoresis --; 1. Analytical and Preparative Methodology --; The Principle of Preparative Capillary Isotachophoresis /; Preparative Capillary Isotachophoresis: Separation of μg Amounts of Some Human Serum Proteins /; Quantitative Determination of Picomoles ADP by Means of Steady State Mixed Zones in Isotachophoresis /; Thin-Layer Polyacrylamide Gel Isotachophoresis, Crossed Immuno-Isotachophoresis and Crossed Isotachophoresis-Electrofocusing /; Isotachophoresis of the Cerebrospinal Fluid (CSF) Proteins: Advantage of Prior Dialysis and its Practical Realization /; 2. Applications --; Plasma Protein Fractionation in Sephadex by Isotachophoresis Using Discrete Spacers /; Immuno-lsoelectric Focusing Analysis of Antibodies Fractionated by Isotachophoresis /; Analytical and Preparative Isotachophoresis in Column and Flat-Bed Gels for Fractionation of Antibodies /; Index --; Backmatter
• Format: Mode of access: Internet via World Wide Web.
• ISBN: 9783110873870
• DOI: 10.1515/9783110873870
• Access: restricted access
• Hierarchical level: Monograph
• Statement of Responsibility: B. J. Radola, D. Graesslin.