Electrofocusing and Isotachophoresis : : Proceedings of the International Symposium, August 1-4, 1976, Hamburg, Germany / / B. J. Radola, D. Graesslin.
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| Place / Publishing House: | Berlin ;, Boston : : De Gruyter, , [2019] ©1977 |
| Year of Publication: | 2019 |
| Edition: | Reprint 2019 |
| Language: | German |
| Online Access: | |
| Physical Description: | 1 online resource (624 p.) |
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| LEADER | 09936nam a22005775i 4500 | ||
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| 001 | 9783110873870 | ||
| 003 | DE-B1597 | ||
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| 024 | 7 | |a 10.1515/9783110873870 |2 doi | |
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| 035 | |a (OCoLC)1110712791 | ||
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| 245 | 0 | 0 | |a Electrofocusing and Isotachophoresis : |b Proceedings of the International Symposium, August 1-4, 1976, Hamburg, Germany / |c B. J. Radola, D. Graesslin. |
| 250 | |a Reprint 2019 | ||
| 264 | 1 | |a Berlin ; |a Boston : |b De Gruyter, |c [2019] | |
| 264 | 4 | |c ©1977 | |
| 300 | |a 1 online resource (624 p.) | ||
| 336 | |a text |b txt |2 rdacontent | ||
| 337 | |a computer |b c |2 rdamedia | ||
| 338 | |a online resource |b cr |2 rdacarrier | ||
| 347 | |a text file |b PDF |2 rda | ||
| 505 | 0 | 0 | |t Frontmatter -- |t Preface -- |t Contents -- |t Contributors -- |t Part I. Isoelectric Focusing -- |t 1. Theoretical and General Aspects -- |t Reminiscences about the Genesis of Isoelectric Focusing and Generalization of the Idea / |r Kolin, A. -- |t Stable pH Gradients - A Key Problem in Isoelectric Focusing / |r Rilbe, H. -- |t Electrofocusing in Buffers: Formation of Natural pH Gradients, Flexibility, Gradient Stability, Relation to Isotachophoresis and Preparative Potential / |r Chrambach, A. / Nguyen, Nga Y. -- |t Comparison of pH Gradients Used for Isoelectric Focusing / |r Fawcett, J. S. -- |t On the Temperature Dependence of Isoelectric Points of Proteins with Special Reference to Isoelectric Focusing / |r Fredriksson, S. -- |t 2. Carrier Ampholytes -- |t Carrier Ampholyte Distribution / |r Brown, R. K. / Caspers, M. L. / Vinogradow, S. N. -- |t Characterization of Carrier Ampholytes by New Visualization Reactions and Chromatographic Methods / |r Radola, B. J. / Tschesche, H. / Schuricht, H. -- |t Synthesis of Carrier Ampholytes for Isoelectric Focusing Containing Sulfonic and Phosphonic Acid Groups Covering a Wide pH Range / |r Grubhofer, N. / Borja, C. -- |t 3. Analytical Methodology -- |t Staining of Proteins in Polyacrylamide Gels / |r Vesterberg, O. / Hansén, L. -- |t Isoelectric Focusing of Complex Protein Mixtures in the Nanogram Range and Enzyme Kinetics of Dehydrogenases Following IEF / |r Bispink, G. / Neuhoff, V. -- |t Soft Laser Scanning Densitometer Compatible with the High Resolution Obtained by Electrofocusing / |r Zeineh, R. A. -- |t Immunocore Electrofocusing: A Separation and Detection Technique Amenable to Scanning Densitometry / |r Zeineh, R. A. -- |t Rapid, Convenient and Economical Procedures for the Determination of the Isoelectric Spectra of Proteins / |r Leaback, D. H. -- |t A Simple Method of Choosing Optimum pH-Conditions for Electrophoresis / |r Rosengren, Å. / Bjellqvist, B. / Gasparic, V. -- |t pH Determinations in Isoelectric Focusing with an Iridium Electrode / |r Gianazza, E. / Righetti, P. G. / Bordi, S. / Papeschi, G. -- |t Anomalous Behaviour of Horseradish Peroxidase in Isoelectric Focusing / |r Delincée, H. / Radola, B. J. -- |t Does Lipoprotein Lipase Bind Ampholytes? / |r Bengtsson, G. / Olivecrona, T. -- |t 4. Biochemical Applications -- |t Zein: Macromolecular Properties, Biosynthesis and Genetic Regulation / |r Righetti, P. G. / Gianazza, E. / Salamini, F. / Galante, E. / Viotti, A. / Soave, C. -- |t Growth and Isoelectric Patterns of Peroxidase in Tobacco Tissue Cultures Under the Influence of Growth Regulator Systems / |r Rücker, W. / Markotai, J. -- |t A Method for Simultaneous Analysis of Several Genetic Loci in Mice After Electrofocusing / |r Narayanan, K. R. / Ray, A. S. -- |t Phylogenetic Differences in Isoelectric Components of Liver Acid Phosphatase and LDH Between Genera of Two Closely Related Rodent Families (Muridae and Microtidae) / |r Kubicz, A. / Wolanska, L. -- |t Fractionation of Nucleic Acids on Isoelectric Focusing / |r Drysdale, J. W. -- |t 5. Clinical Applications -- |t Polyacrylamide Gel Isoelectric Focusing of Alpha-1-Proteinase Inhibitors (α1 Pi) Phenotypes: I. Optimal Condition, Histochemical and Proteinase Probe Studies / |r Allen, R. C. / Oulla, P. M. / Arnaud, P. / Baumstark, J. S. -- |t Polyacrylamide Gel Isoelectric Focusing (PAGIF) Applied to Alpha-1-Proteinase Inhibitor (α1 Pi) Phenotyping: II. Immunochemical Procedures / |r Arnaud, P. / Chapuis-Cellier, C. / Wilson, G. B. / Koistinen, J. / Allen, R. C. / Fudenberg, H. H. -- |t High Resolution Phenotyping of α1-Antitrypsins by Thin-Layer Polyacrylamide Electrofocusing / |r Jeppsson, J. O. -- |t Broad-Beta Disease (Hyperlipoproteinaemia Type III): Genetics, Gene Frequency and Diagnosis without Ultracentrifugation / |r Utermann, G. / Hess, M. / Vogelberg, K. H. -- |t Screening for Abnormal Hemoglobins in the Newborn: A Highly Economic Procedure Using Isoelectric Focusing / |r Altland, K. -- |t The Crystallins of the Aging Lens from Five Species Studies by Various Methods of Thin-Layer Isoelectric Focusing / |r Bours, J. -- |t Separation of Erythrocyte Membrane Components in Electric Field. Polyacrylamide Gel Isoelectric Focusing: A Potential Method for the Diagnosis of Membrane Abnormality / |r Das, P. K. / Graesslin, D. / Goedde, H. W. -- |t Microheterogeneity of Serum Glycoproteins as Revealed by Flat-Bed Gel Isoelectric Focusing / |r Hamann, A. -- |t Diagnosis of Cystic Fibrosis (CF) - Purification and Distinction Between CF Protein and Ciliary Dyskinesia Activity in CF and Asthmatic Sera Using Isoelectric Focusing and the Rabbit Tracheal Ciliary Bioassay / |r Wilson, G. B. / Monsher, M. T. / Arnaud, P. / Fudenberg, H. H. -- |t Microheterogeneity of Human Kininogen, Alpha- 2-HS and Alpha-1-Acid Glycoproteins by Thin- Layer Isoelectric Focusing / |r Hamberg, U. / Turpeinen, U. / Knuutinen, U. -- |t A Genetic Variant of Amylase from Human Parotid Saliva Detected by Isoelectric Focusing / |r Pronk, J. C. -- |t 6. Two-Dimensional Techniques -- |t Biological Applications of Two-Dimensional Gel Electrophoresis / |r Ivarie, R. D. / Gelfand, D. H. / Jones, P. P. / O'Farrell, P. Z. / Polisky, B. A. / Steinberg, R. A. / O'Farrell, P. H. -- |t Plant Proteins Evaluated by Two-Dimensional Methods / |r Stegemann, H. -- |t Two-Dimensional Separation of Lymphocyte Microsomal Membrane Proteins: Isoelectric Focusing Linked to SDS-Polyacrylamide Gel Electrophoresis / |r Kniifermann, H. -- |t Mammalian Mitochondrial Ribosomes: Two- Dimensional Gel Electrophoresis of the 55 S- and the Corresponding Subunit Proteins / |r Czempiel, W. -- |t Crossed Immunoelectrofocusing for Standardization and Characterization of Fungal Antigens / |r Holmberg, K. / Wadström, T. -- |t 7. Preparative Separations -- |t Isoelectric Focusing in Free Water / |r Denckla, W. D. -- |t Preparative Electrofocusing in Flat-Beds of Granulated Gel - Methodological Aspects / |r Winter, A. -- |t Preparative Scale Purification of Bacterial Enzymes and Toxins by Isoelectric Focusing and Isotachophoresis / |r Wadstrom, T. / Möllby, R. / Olsson, B. / Söderholm, J. / Smyth, C. J. -- |t Preparative Zone Convection Electrofocusing in Helical Glass Tubes / |r Quast, R. -- |t Chromatofocusing: Isoelectric Focusing on Ion Exchangers in the Absence of an Externally Applied Potential / |r Sluyterman, L. A. A. E. / Wijdenes, J. -- |t 8. Separation of Cells -- |t New Instrumentation and Procedures for the Analysis of Mammalian Cells by Electrophoretic Techniques / |r Catsimpoolas, N. / Griffith, A. L. -- |t Continuous-Flow Isoelectric Focusing: Further Studies on Human Red Blood Cells and on the Separation of Rat Liver Light Mitochondrial Fraction / |r Just, W. W. / Werner, G. -- |t Preparative Electrofocusing of Native and Modified Living Mammalian Cells in a Stationary Ficoll/ Sucrose Gradient / |r Manske, W. / Bohn, B. / Brossmer, R. -- |t Part II. Isotachophoresis -- |t 1. Analytical and Preparative Methodology -- |t The Principle of Preparative Capillary Isotachophoresis / |r Arlinger, L. -- |t Preparative Capillary Isotachophoresis: Separation of μg Amounts of Some Human Serum Proteins / |r Moberg, U. / Hjalmarsson, S. G. / Arlinger, L. / Lundin, H. -- |t Quantitative Determination of Picomoles ADP by Means of Steady State Mixed Zones in Isotachophoresis / |r Wielders, J. P. M. / Everaerts, F. M. -- |t Thin-Layer Polyacrylamide Gel Isotachophoresis, Crossed Immuno-Isotachophoresis and Crossed Isotachophoresis-Electrofocusing / |r Brogren, C. H. -- |t Isotachophoresis of the Cerebrospinal Fluid (CSF) Proteins: Advantage of Prior Dialysis and its Practical Realization / |r Delmotte, P. -- |t 2. Applications -- |t Plasma Protein Fractionation in Sephadex by Isotachophoresis Using Discrete Spacers / |r Bier, M. / Kopwillem, A. -- |t Immuno-lsoelectric Focusing Analysis of Antibodies Fractionated by Isotachophoresis / |r Peltre, G. / Brogren, C. H. -- |t Analytical and Preparative Isotachophoresis in Column and Flat-Bed Gels for Fractionation of Antibodies / |r Brogren, C. H. / Peltre, G. -- |t Index -- |t Backmatter |
| 506 | 0 | |a restricted access |u http://purl.org/coar/access_right/c_16ec |f online access with authorization |2 star | |
| 538 | |a Mode of access: Internet via World Wide Web. | ||
| 546 | |a In German. | ||
| 588 | 0 | |a Description based on online resource; title from PDF title page (publisher's Web site, viewed 23. Jul 2019) | |
| 650 | 7 | |a SCIENCE / General. |2 bisacsh | |
| 700 | 1 | |a Graesslin, D., |e editor. | |
| 700 | 1 | |a Radola, B. J., |e editor. | |
| 776 | 0 | |c print |z 9783110070262 | |
| 856 | 4 | 0 | |u https://doi.org/10.1515/9783110873870 |
| 856 | 4 | 2 | |3 Cover |u https://www.degruyter.com/cover/covers/9783110873870.jpg |
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