The Impact of Food Bioactives on Health : : In Vitro and Ex Vivo Models.
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Verhoeckx, Kitty. The Impact of Food Bioactives on Health : In Vitro and Ex Vivo Models. 1st ed. Cham : Springer International Publishing AG, 2015. ©2015. 1 online resource (341 pages) text txt rdacontent computer c rdamedia online resource cr rdacarrier Intro -- Preface -- General Introduction -- Digestion and Absorption -- Cells Present in the Intestine -- Role of Microbiota -- Contents -- Part I: Gastrointestinal Digestion Models, General Introduction -- General Introduction -- References -- Chapter 1: Static Digestion Models: General Introduction -- 1.1 Definition of Concepts: Bioavailability, Bioaccessibility and Bioactivity -- 1.2 Static Methods -- 1.2.1 Solubility/Dialyzability -- 1.2.2 Digestion Conditions -- 1.3 Applications: Advantages and Disadvantages -- 1.4 Static Versus In Vivo Digestion: Conclusions -- References -- Chapter 2: InfoGest Consensus Method -- 2.1 Introduction -- 2.2 The Oral Phase -- 2.3 The Gastric Phase -- 2.4 The Small Intestinal Phase -- 2.5 Practicalities -- 2.6 Sampling -- 2.7 Conclusions -- References -- Chapter 3: Approaches to Static Digestion Models -- 3.1 Introduction -- 3.2 Static Models for Protein Hydrolysis -- 3.3 Static Models for Lipid Hydrolysis -- 3.4 Other Static Models -- References -- Chapter 4: Dynamic Digestion Models: General Introduction -- 4.1 Geometry -- 4.2 Physical Forces -- 4.3 Biochemistry -- References -- Chapter 5: The TNO Gastro-Intestinal Model (TIM) -- 5.1 Introduction -- 5.2 Concept of TIM -- 5.3 TIM-1 -- 5.4 TinyTIM -- 5.5 Advanced Gastric Compartment (TIM-agc) -- 5.6 The Use of TIM to Study the Bio-accessibility of Nutrients -- 5.7 Protein Quality -- 5.8 Prediction of Glycemic Response -- 5.9 Lipids -- 5.10 Conclusions -- References -- Chapter 6: Dynamic Gastric Model (DGM) -- 6.1 Origins and Design of the DGM -- 6.2 General Protocol for DGM Experiments -- 6.3 Uses of the DGM -- 6.3.1 Food-Based Research -- 6.3.2 Pharmaceutical-Based Research -- 6.4 Advantages, Disadvantages and Limitations -- 6.5 Availability of the System -- References -- Chapter 7: Human Gastric Simulator (Riddet Model) -- 7.1 Origins of the HGS. 7.2 Model Description -- 7.2.1 Gastric Compartment -- 7.2.2 Gastric Motility -- 7.2.3 Gastric Emptying -- 7.2.4 Gastric Secretions -- 7.2.5 Temperature Control -- 7.3 Analysis of HGS Biomechanical Relevance -- 7.4 Operating Protocol -- 7.4.1 Preparation of a Food Bolus -- 7.4.2 Gastric Processing -- 7.5 Uses of the HGS -- 7.5.1 Role of ACW Activity on Food Digestion -- 7.5.2 Role of Food Material Properties -- 7.6 Advantages and Limitations -- 7.7 Availability of the System -- References -- Chapter 8: The DIDGI® System -- 8.1 Origins and Design of the DIDGI® System -- 8.2 Validation of DIDGI® for the Digestion of Infant Formula -- 8.2.1 Protocol for the In Vitro Dynamic Digestion of Infant Formula Using the DIDGI® System -- 8.2.2 In Vivo Digestion of Infant Formula on Piglets -- 8.2.3 Comparison In Vitro/In Vivo Data -- 8.3 Advantages, Disadvantages and Limitations -- 8.4 Conclusion and Prospects -- References -- Part II: General Introduction to Cells, Cell Lines and Cell Culture -- Introduction -- Salt Solutions -- Culture Media -- Medium Quality -- pH and CO2 -- Serum Addition -- The Cell Culture Hood -- Cell Culture Terminology (Fig. 1) -- General Cell Culture Protocols -- Trypsinisation and Subculturing of Cells -- Passaging of Cells in Suspension Culture -- Freezing Cells -- The Thawing and Recovery of Cells -- Cell Viability Testing -- Contamination of Cell Cultures -- References -- Chapter 9: Epithelial Cell Models -- General Introduction -- 9.1 Measurement of Transepithelial Electrical Resistance (TEER) -- 9.1.1 Basic Protocol -- 9.1.2 Calculating Transepithelial Resistance -- 9.2 Verification of Monolayer Integrity by Lucifer Yellow Flux -- 9.2.1 Basic Protocol -- 9.3 Summary -- References -- Chapter 10: Caco-2 Cell Line -- 10.1 Origin -- 10.2 Features and Mechanisms -- 10.3 Stability, Consistency and Reproducibility. 10.4 Relevance to Human In Vivo Situation -- 10.5 General Protocols for Caco-2 Cells -- 10.5.1 General Maintenance -- 10.5.2 Protocol for Polarizing Caco-2 Cells in Tissue Culture Inserts -- 10.5.3 Troubleshooting Guide for Transport Experiments Across Caco-2 Monolayers -- 10.6 Applications -- 10.7 Advantages and Disadvantages -- 10.8 Conclusion -- References -- Chapter 11: HT29 Cell Line -- 11.1 Origin -- 11.2 Features and Mechanisms -- 11.3 Stability, Consistency and Reproducibility -- 11.4 Relevance to Human In Vivo Situation -- 11.5 General Protocol for HT29-MTX Cells -- 11.5.1 Cell Maintenance Protocol -- 11.5.2 Experimental Protocol for Test Compounds -- 11.5.2.1 Study of the Mucin-Stimulating Activity -- 11.5.2.2 Evaluation of Transepithelial Absorption by Transwell® Inserts -- 11.6 Experimental Read Out -- 11.6.1 Functionality Studies -- 11.6.2 Transport Studies -- 11.6.3 Microorganisms Survival, Adhesion or Invasion -- 11.7 Conclusions -- References -- Chapter 12: The IPEC-J2 Cell Line -- 12.1 Origin -- 12.2 Special Features/Morphology/Receptors -- 12.3 Stability/Consistency/Reproducibility of the System -- 12.4 Relevance to Human In Vivo Situation -- 12.5 General Protocol -- 12.5.1 Culture Conditions -- 12.5.2 Experimental Readout -- 12.5.3 Sample Preparation -- 12.6 Conclusion -- References -- Chapter 13: Co-cultivation of Caco-2 and HT-29MTX -- 13.1 Origin, Features and Mechanisms -- 13.2 Stability/Consistency and Reproducibility -- 13.3 Relevance to the Human In Vivo Situation -- 13.4 General Protocol -- 13.5 Assess Viability -- 13.6 Experimental Readout -- 13.7 Advantages, Disadvantages and Limitations -- 13.8 Conclusions -- References -- Part III: Innate and Adaptive Immune Cells: General Introduction Iván López-Expósito -- Monocytes and Macrophages -- Dendritic Cells -- Human Peripheral Blood Mononuclear Cells. T Lymphocytes or T-Cells -- References -- Chapter 14: THP-1 and U937 Cells -- 14.1 Origin and Some Features of THP-1 and U937 Cells -- 14.2 Stability, Consistency and Reproducibility of the System -- 14.3 Relevance to Human In Vivo Situation -- 14.4 Other Models with the Same Applicability -- 14.5 General Protocol of Culturing THP-1 Cells -- 14.6 Differentiation of THP-1 and U937 Monocytes into Macrophages -- 14.7 Differentiation of THP-1 and U937 Monocytes into Dendritic Cells -- 14.8 Controls to Test Viability and Performance of the Model -- 14.9 Critical Notes -- 14.10 Read-Out of the System -- References -- Chapter 15: Peripheral Blood Mononuclear Cells -- 15.1 Origin -- 15.2 Features and Mechanisms -- 15.3 Stability, Consistency and Reproducibility -- 15.4 Relevance to Human In Vivo Situation -- 15.5 General Protocol -- 15.5.1 Study of Proliferative/Cytotoxic Activity -- 15.5.2 Study of Inflammatory Responses -- 15.6 Assess Viability -- 15.7 Experimental Read Out -- 15.8 Advantages, Disadvantages and Limitations of the System -- 15.9 Conclusions -- References -- Chapter 16: PBMC-Derived T Cells -- 16.1 Introduction and Origin -- 16.2 Features and Mechanisms -- 16.3 Applications of T Cell Cultures -- 16.4 General Protocol -- 16.4.1 T Cell Isolation Protocols -- 16.4.2 Indirect Positive Isolation of Human CD4+ T -- 16.4.2.1 Preparation of Cells and Antibodies -- 16.4.2.2 Coating of PBMCs with CD4 Antibody -- 16.4.2.3 Magnetic Beads Washing Procedure -- 16.4.2.4 Separation of T Cells -- 16.4.2.5 Detachment of T Cells from Beads -- 16.5 Assess Viability -- 16.6 Samples -- 16.7 Experimental Readouts -- References -- Chapter 17: Dendritic Cells -- 17.1 Origin -- 17.2 Features and Mechanisms -- 17.2.1 DC Subsets -- 17.2.1.1 Blood DCs -- 17.2.1.2 Mucosal DCs -- 17.2.1.3 Monocyte-Derived DCs -- 17.3 General Protocols -- 17.3.1 DC Cell Lines. 17.3.2 Isolating Primary DCs from Blood -- 17.3.3 CD34+-Derived DCs -- 17.3.4 Monocyte-Derived DCs -- 17.4 Asses Viability -- 17.5 Experimental Readout -- 17.5.1 Co-stimulation -- 17.5.2 Cytokine Production -- 17.5.3 Other DC Readouts -- 17.6 In Vitro Studies on Food Bioactives Using DCs (Table 17.3) -- 17.7 Critical Notes -- References -- Chapter 18: Co-culture Caco-2/Immune Cells -- 18.1 Origin, Features and Mechanisms -- 18.2 Relevance to Human In Vivo Situation -- 18.2.1 Co-culture Caco-2 and Dendritic Cells -- 18.2.2 Co-culture Caco-2 and B-cells (Raji) -- 18.3 Stability, Consistency and Reproducibility -- 18.4 General Protocol -- 18.4.1 Co-culture of Caco-2/Human Monocyte Derived DCs (Include Contact Dependent Events) -- 18.4.2 Caco-2/Human Monocyte Derived DCs (Soluble Factors) -- 18.4.3 Caco-2/THP-1 (Soluble Factors) -- 18.4.4 Caco-2/PBMCs (Soluble Factors) -- 18.4.5 Caco-2/B Cells -- 18.5 Assess Viability -- 18.6 Experimental Readout -- 18.7 Advantages, Disadvantages and Limitations -- 18.8 Conclusions -- References -- Part IV: Enteroendocrine Cell Models: General Introduction -- References -- Chapter 19: STC-1 Cells -- 19.1 Origin -- 19.2 Features and Mechanisms -- 19.3 Stability, Consistency and Reproducibility -- 19.4 Relevance to Human In Vivo Situation -- 19.5 General Protocol -- 19.5.1 Cell Maintenance Protocol -- 19.5.2 Experimental Protocol for Test Compounds -- 19.6 Assess Viability -- 19.7 Experimental Read out -- 19.8 Conclusions -- References -- Chapter 20: NCI-H716 Cells -- 20.1 Introduction -- 20.2 Origin -- 20.3 Features and Mechanisms -- 20.4 Stability/Consistency/Reproducibility -- 20.5 Relevance to the Human L-Cell In Vivo -- 20.6 General Protocol -- 20.6.1 Cell Maintenance Protocol -- 20.6.2 Experimental Protocol for Test Compounds -- 20.7 Assess Viability -- 20.8 Experimental Readout -- 20.9 Conclusions -- References. Chapter 21: Murine GLUTag Cells. Description based on publisher supplied metadata and other sources. Electronic reproduction. Ann Arbor, Michigan : ProQuest Ebook Central, 2024. Available via World Wide Web. Access may be limited to ProQuest Ebook Central affiliated libraries. Electronic books. Cotter, Paul. López-Expósito, Iván. Kleiveland, Charlotte. Lea, Tor. Mackie, Alan. Requena, Teresa. Swiatecka, Dominika. Wichers, Harry. Print version: Verhoeckx, Kitty The Impact of Food Bioactives on Health Cham : Springer International Publishing AG,c2015 9783319157917 ProQuest (Firm) https://ebookcentral.proquest.com/lib/oeawat/detail.action?docID=6422901 Click to View |
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Verhoeckx, Kitty. The Impact of Food Bioactives on Health : In Vitro and Ex Vivo Models. Intro -- Preface -- General Introduction -- Digestion and Absorption -- Cells Present in the Intestine -- Role of Microbiota -- Contents -- Part I: Gastrointestinal Digestion Models, General Introduction -- General Introduction -- References -- Chapter 1: Static Digestion Models: General Introduction -- 1.1 Definition of Concepts: Bioavailability, Bioaccessibility and Bioactivity -- 1.2 Static Methods -- 1.2.1 Solubility/Dialyzability -- 1.2.2 Digestion Conditions -- 1.3 Applications: Advantages and Disadvantages -- 1.4 Static Versus In Vivo Digestion: Conclusions -- References -- Chapter 2: InfoGest Consensus Method -- 2.1 Introduction -- 2.2 The Oral Phase -- 2.3 The Gastric Phase -- 2.4 The Small Intestinal Phase -- 2.5 Practicalities -- 2.6 Sampling -- 2.7 Conclusions -- References -- Chapter 3: Approaches to Static Digestion Models -- 3.1 Introduction -- 3.2 Static Models for Protein Hydrolysis -- 3.3 Static Models for Lipid Hydrolysis -- 3.4 Other Static Models -- References -- Chapter 4: Dynamic Digestion Models: General Introduction -- 4.1 Geometry -- 4.2 Physical Forces -- 4.3 Biochemistry -- References -- Chapter 5: The TNO Gastro-Intestinal Model (TIM) -- 5.1 Introduction -- 5.2 Concept of TIM -- 5.3 TIM-1 -- 5.4 TinyTIM -- 5.5 Advanced Gastric Compartment (TIM-agc) -- 5.6 The Use of TIM to Study the Bio-accessibility of Nutrients -- 5.7 Protein Quality -- 5.8 Prediction of Glycemic Response -- 5.9 Lipids -- 5.10 Conclusions -- References -- Chapter 6: Dynamic Gastric Model (DGM) -- 6.1 Origins and Design of the DGM -- 6.2 General Protocol for DGM Experiments -- 6.3 Uses of the DGM -- 6.3.1 Food-Based Research -- 6.3.2 Pharmaceutical-Based Research -- 6.4 Advantages, Disadvantages and Limitations -- 6.5 Availability of the System -- References -- Chapter 7: Human Gastric Simulator (Riddet Model) -- 7.1 Origins of the HGS. 7.2 Model Description -- 7.2.1 Gastric Compartment -- 7.2.2 Gastric Motility -- 7.2.3 Gastric Emptying -- 7.2.4 Gastric Secretions -- 7.2.5 Temperature Control -- 7.3 Analysis of HGS Biomechanical Relevance -- 7.4 Operating Protocol -- 7.4.1 Preparation of a Food Bolus -- 7.4.2 Gastric Processing -- 7.5 Uses of the HGS -- 7.5.1 Role of ACW Activity on Food Digestion -- 7.5.2 Role of Food Material Properties -- 7.6 Advantages and Limitations -- 7.7 Availability of the System -- References -- Chapter 8: The DIDGI® System -- 8.1 Origins and Design of the DIDGI® System -- 8.2 Validation of DIDGI® for the Digestion of Infant Formula -- 8.2.1 Protocol for the In Vitro Dynamic Digestion of Infant Formula Using the DIDGI® System -- 8.2.2 In Vivo Digestion of Infant Formula on Piglets -- 8.2.3 Comparison In Vitro/In Vivo Data -- 8.3 Advantages, Disadvantages and Limitations -- 8.4 Conclusion and Prospects -- References -- Part II: General Introduction to Cells, Cell Lines and Cell Culture -- Introduction -- Salt Solutions -- Culture Media -- Medium Quality -- pH and CO2 -- Serum Addition -- The Cell Culture Hood -- Cell Culture Terminology (Fig. 1) -- General Cell Culture Protocols -- Trypsinisation and Subculturing of Cells -- Passaging of Cells in Suspension Culture -- Freezing Cells -- The Thawing and Recovery of Cells -- Cell Viability Testing -- Contamination of Cell Cultures -- References -- Chapter 9: Epithelial Cell Models -- General Introduction -- 9.1 Measurement of Transepithelial Electrical Resistance (TEER) -- 9.1.1 Basic Protocol -- 9.1.2 Calculating Transepithelial Resistance -- 9.2 Verification of Monolayer Integrity by Lucifer Yellow Flux -- 9.2.1 Basic Protocol -- 9.3 Summary -- References -- Chapter 10: Caco-2 Cell Line -- 10.1 Origin -- 10.2 Features and Mechanisms -- 10.3 Stability, Consistency and Reproducibility. 10.4 Relevance to Human In Vivo Situation -- 10.5 General Protocols for Caco-2 Cells -- 10.5.1 General Maintenance -- 10.5.2 Protocol for Polarizing Caco-2 Cells in Tissue Culture Inserts -- 10.5.3 Troubleshooting Guide for Transport Experiments Across Caco-2 Monolayers -- 10.6 Applications -- 10.7 Advantages and Disadvantages -- 10.8 Conclusion -- References -- Chapter 11: HT29 Cell Line -- 11.1 Origin -- 11.2 Features and Mechanisms -- 11.3 Stability, Consistency and Reproducibility -- 11.4 Relevance to Human In Vivo Situation -- 11.5 General Protocol for HT29-MTX Cells -- 11.5.1 Cell Maintenance Protocol -- 11.5.2 Experimental Protocol for Test Compounds -- 11.5.2.1 Study of the Mucin-Stimulating Activity -- 11.5.2.2 Evaluation of Transepithelial Absorption by Transwell® Inserts -- 11.6 Experimental Read Out -- 11.6.1 Functionality Studies -- 11.6.2 Transport Studies -- 11.6.3 Microorganisms Survival, Adhesion or Invasion -- 11.7 Conclusions -- References -- Chapter 12: The IPEC-J2 Cell Line -- 12.1 Origin -- 12.2 Special Features/Morphology/Receptors -- 12.3 Stability/Consistency/Reproducibility of the System -- 12.4 Relevance to Human In Vivo Situation -- 12.5 General Protocol -- 12.5.1 Culture Conditions -- 12.5.2 Experimental Readout -- 12.5.3 Sample Preparation -- 12.6 Conclusion -- References -- Chapter 13: Co-cultivation of Caco-2 and HT-29MTX -- 13.1 Origin, Features and Mechanisms -- 13.2 Stability/Consistency and Reproducibility -- 13.3 Relevance to the Human In Vivo Situation -- 13.4 General Protocol -- 13.5 Assess Viability -- 13.6 Experimental Readout -- 13.7 Advantages, Disadvantages and Limitations -- 13.8 Conclusions -- References -- Part III: Innate and Adaptive Immune Cells: General Introduction Iván López-Expósito -- Monocytes and Macrophages -- Dendritic Cells -- Human Peripheral Blood Mononuclear Cells. T Lymphocytes or T-Cells -- References -- Chapter 14: THP-1 and U937 Cells -- 14.1 Origin and Some Features of THP-1 and U937 Cells -- 14.2 Stability, Consistency and Reproducibility of the System -- 14.3 Relevance to Human In Vivo Situation -- 14.4 Other Models with the Same Applicability -- 14.5 General Protocol of Culturing THP-1 Cells -- 14.6 Differentiation of THP-1 and U937 Monocytes into Macrophages -- 14.7 Differentiation of THP-1 and U937 Monocytes into Dendritic Cells -- 14.8 Controls to Test Viability and Performance of the Model -- 14.9 Critical Notes -- 14.10 Read-Out of the System -- References -- Chapter 15: Peripheral Blood Mononuclear Cells -- 15.1 Origin -- 15.2 Features and Mechanisms -- 15.3 Stability, Consistency and Reproducibility -- 15.4 Relevance to Human In Vivo Situation -- 15.5 General Protocol -- 15.5.1 Study of Proliferative/Cytotoxic Activity -- 15.5.2 Study of Inflammatory Responses -- 15.6 Assess Viability -- 15.7 Experimental Read Out -- 15.8 Advantages, Disadvantages and Limitations of the System -- 15.9 Conclusions -- References -- Chapter 16: PBMC-Derived T Cells -- 16.1 Introduction and Origin -- 16.2 Features and Mechanisms -- 16.3 Applications of T Cell Cultures -- 16.4 General Protocol -- 16.4.1 T Cell Isolation Protocols -- 16.4.2 Indirect Positive Isolation of Human CD4+ T -- 16.4.2.1 Preparation of Cells and Antibodies -- 16.4.2.2 Coating of PBMCs with CD4 Antibody -- 16.4.2.3 Magnetic Beads Washing Procedure -- 16.4.2.4 Separation of T Cells -- 16.4.2.5 Detachment of T Cells from Beads -- 16.5 Assess Viability -- 16.6 Samples -- 16.7 Experimental Readouts -- References -- Chapter 17: Dendritic Cells -- 17.1 Origin -- 17.2 Features and Mechanisms -- 17.2.1 DC Subsets -- 17.2.1.1 Blood DCs -- 17.2.1.2 Mucosal DCs -- 17.2.1.3 Monocyte-Derived DCs -- 17.3 General Protocols -- 17.3.1 DC Cell Lines. 17.3.2 Isolating Primary DCs from Blood -- 17.3.3 CD34+-Derived DCs -- 17.3.4 Monocyte-Derived DCs -- 17.4 Asses Viability -- 17.5 Experimental Readout -- 17.5.1 Co-stimulation -- 17.5.2 Cytokine Production -- 17.5.3 Other DC Readouts -- 17.6 In Vitro Studies on Food Bioactives Using DCs (Table 17.3) -- 17.7 Critical Notes -- References -- Chapter 18: Co-culture Caco-2/Immune Cells -- 18.1 Origin, Features and Mechanisms -- 18.2 Relevance to Human In Vivo Situation -- 18.2.1 Co-culture Caco-2 and Dendritic Cells -- 18.2.2 Co-culture Caco-2 and B-cells (Raji) -- 18.3 Stability, Consistency and Reproducibility -- 18.4 General Protocol -- 18.4.1 Co-culture of Caco-2/Human Monocyte Derived DCs (Include Contact Dependent Events) -- 18.4.2 Caco-2/Human Monocyte Derived DCs (Soluble Factors) -- 18.4.3 Caco-2/THP-1 (Soluble Factors) -- 18.4.4 Caco-2/PBMCs (Soluble Factors) -- 18.4.5 Caco-2/B Cells -- 18.5 Assess Viability -- 18.6 Experimental Readout -- 18.7 Advantages, Disadvantages and Limitations -- 18.8 Conclusions -- References -- Part IV: Enteroendocrine Cell Models: General Introduction -- References -- Chapter 19: STC-1 Cells -- 19.1 Origin -- 19.2 Features and Mechanisms -- 19.3 Stability, Consistency and Reproducibility -- 19.4 Relevance to Human In Vivo Situation -- 19.5 General Protocol -- 19.5.1 Cell Maintenance Protocol -- 19.5.2 Experimental Protocol for Test Compounds -- 19.6 Assess Viability -- 19.7 Experimental Read out -- 19.8 Conclusions -- References -- Chapter 20: NCI-H716 Cells -- 20.1 Introduction -- 20.2 Origin -- 20.3 Features and Mechanisms -- 20.4 Stability/Consistency/Reproducibility -- 20.5 Relevance to the Human L-Cell In Vivo -- 20.6 General Protocol -- 20.6.1 Cell Maintenance Protocol -- 20.6.2 Experimental Protocol for Test Compounds -- 20.7 Assess Viability -- 20.8 Experimental Readout -- 20.9 Conclusions -- References. Chapter 21: Murine GLUTag Cells. |
author_facet |
Verhoeckx, Kitty. Cotter, Paul. López-Expósito, Iván. Kleiveland, Charlotte. Lea, Tor. Mackie, Alan. Requena, Teresa. Swiatecka, Dominika. Wichers, Harry. |
author_variant |
k v kv |
author2 |
Cotter, Paul. López-Expósito, Iván. Kleiveland, Charlotte. Lea, Tor. Mackie, Alan. Requena, Teresa. Swiatecka, Dominika. Wichers, Harry. |
author2_variant |
p c pc i l e ile c k ck t l tl a m am t r tr d s ds h w hw |
author2_role |
TeilnehmendeR TeilnehmendeR TeilnehmendeR TeilnehmendeR TeilnehmendeR TeilnehmendeR TeilnehmendeR TeilnehmendeR |
author_sort |
Verhoeckx, Kitty. |
title |
The Impact of Food Bioactives on Health : In Vitro and Ex Vivo Models. |
title_sub |
In Vitro and Ex Vivo Models. |
title_full |
The Impact of Food Bioactives on Health : In Vitro and Ex Vivo Models. |
title_fullStr |
The Impact of Food Bioactives on Health : In Vitro and Ex Vivo Models. |
title_full_unstemmed |
The Impact of Food Bioactives on Health : In Vitro and Ex Vivo Models. |
title_auth |
The Impact of Food Bioactives on Health : In Vitro and Ex Vivo Models. |
title_new |
The Impact of Food Bioactives on Health : |
title_sort |
the impact of food bioactives on health : in vitro and ex vivo models. |
publisher |
Springer International Publishing AG, |
publishDate |
2015 |
physical |
1 online resource (341 pages) |
edition |
1st ed. |
contents |
Intro -- Preface -- General Introduction -- Digestion and Absorption -- Cells Present in the Intestine -- Role of Microbiota -- Contents -- Part I: Gastrointestinal Digestion Models, General Introduction -- General Introduction -- References -- Chapter 1: Static Digestion Models: General Introduction -- 1.1 Definition of Concepts: Bioavailability, Bioaccessibility and Bioactivity -- 1.2 Static Methods -- 1.2.1 Solubility/Dialyzability -- 1.2.2 Digestion Conditions -- 1.3 Applications: Advantages and Disadvantages -- 1.4 Static Versus In Vivo Digestion: Conclusions -- References -- Chapter 2: InfoGest Consensus Method -- 2.1 Introduction -- 2.2 The Oral Phase -- 2.3 The Gastric Phase -- 2.4 The Small Intestinal Phase -- 2.5 Practicalities -- 2.6 Sampling -- 2.7 Conclusions -- References -- Chapter 3: Approaches to Static Digestion Models -- 3.1 Introduction -- 3.2 Static Models for Protein Hydrolysis -- 3.3 Static Models for Lipid Hydrolysis -- 3.4 Other Static Models -- References -- Chapter 4: Dynamic Digestion Models: General Introduction -- 4.1 Geometry -- 4.2 Physical Forces -- 4.3 Biochemistry -- References -- Chapter 5: The TNO Gastro-Intestinal Model (TIM) -- 5.1 Introduction -- 5.2 Concept of TIM -- 5.3 TIM-1 -- 5.4 TinyTIM -- 5.5 Advanced Gastric Compartment (TIM-agc) -- 5.6 The Use of TIM to Study the Bio-accessibility of Nutrients -- 5.7 Protein Quality -- 5.8 Prediction of Glycemic Response -- 5.9 Lipids -- 5.10 Conclusions -- References -- Chapter 6: Dynamic Gastric Model (DGM) -- 6.1 Origins and Design of the DGM -- 6.2 General Protocol for DGM Experiments -- 6.3 Uses of the DGM -- 6.3.1 Food-Based Research -- 6.3.2 Pharmaceutical-Based Research -- 6.4 Advantages, Disadvantages and Limitations -- 6.5 Availability of the System -- References -- Chapter 7: Human Gastric Simulator (Riddet Model) -- 7.1 Origins of the HGS. 7.2 Model Description -- 7.2.1 Gastric Compartment -- 7.2.2 Gastric Motility -- 7.2.3 Gastric Emptying -- 7.2.4 Gastric Secretions -- 7.2.5 Temperature Control -- 7.3 Analysis of HGS Biomechanical Relevance -- 7.4 Operating Protocol -- 7.4.1 Preparation of a Food Bolus -- 7.4.2 Gastric Processing -- 7.5 Uses of the HGS -- 7.5.1 Role of ACW Activity on Food Digestion -- 7.5.2 Role of Food Material Properties -- 7.6 Advantages and Limitations -- 7.7 Availability of the System -- References -- Chapter 8: The DIDGI® System -- 8.1 Origins and Design of the DIDGI® System -- 8.2 Validation of DIDGI® for the Digestion of Infant Formula -- 8.2.1 Protocol for the In Vitro Dynamic Digestion of Infant Formula Using the DIDGI® System -- 8.2.2 In Vivo Digestion of Infant Formula on Piglets -- 8.2.3 Comparison In Vitro/In Vivo Data -- 8.3 Advantages, Disadvantages and Limitations -- 8.4 Conclusion and Prospects -- References -- Part II: General Introduction to Cells, Cell Lines and Cell Culture -- Introduction -- Salt Solutions -- Culture Media -- Medium Quality -- pH and CO2 -- Serum Addition -- The Cell Culture Hood -- Cell Culture Terminology (Fig. 1) -- General Cell Culture Protocols -- Trypsinisation and Subculturing of Cells -- Passaging of Cells in Suspension Culture -- Freezing Cells -- The Thawing and Recovery of Cells -- Cell Viability Testing -- Contamination of Cell Cultures -- References -- Chapter 9: Epithelial Cell Models -- General Introduction -- 9.1 Measurement of Transepithelial Electrical Resistance (TEER) -- 9.1.1 Basic Protocol -- 9.1.2 Calculating Transepithelial Resistance -- 9.2 Verification of Monolayer Integrity by Lucifer Yellow Flux -- 9.2.1 Basic Protocol -- 9.3 Summary -- References -- Chapter 10: Caco-2 Cell Line -- 10.1 Origin -- 10.2 Features and Mechanisms -- 10.3 Stability, Consistency and Reproducibility. 10.4 Relevance to Human In Vivo Situation -- 10.5 General Protocols for Caco-2 Cells -- 10.5.1 General Maintenance -- 10.5.2 Protocol for Polarizing Caco-2 Cells in Tissue Culture Inserts -- 10.5.3 Troubleshooting Guide for Transport Experiments Across Caco-2 Monolayers -- 10.6 Applications -- 10.7 Advantages and Disadvantages -- 10.8 Conclusion -- References -- Chapter 11: HT29 Cell Line -- 11.1 Origin -- 11.2 Features and Mechanisms -- 11.3 Stability, Consistency and Reproducibility -- 11.4 Relevance to Human In Vivo Situation -- 11.5 General Protocol for HT29-MTX Cells -- 11.5.1 Cell Maintenance Protocol -- 11.5.2 Experimental Protocol for Test Compounds -- 11.5.2.1 Study of the Mucin-Stimulating Activity -- 11.5.2.2 Evaluation of Transepithelial Absorption by Transwell® Inserts -- 11.6 Experimental Read Out -- 11.6.1 Functionality Studies -- 11.6.2 Transport Studies -- 11.6.3 Microorganisms Survival, Adhesion or Invasion -- 11.7 Conclusions -- References -- Chapter 12: The IPEC-J2 Cell Line -- 12.1 Origin -- 12.2 Special Features/Morphology/Receptors -- 12.3 Stability/Consistency/Reproducibility of the System -- 12.4 Relevance to Human In Vivo Situation -- 12.5 General Protocol -- 12.5.1 Culture Conditions -- 12.5.2 Experimental Readout -- 12.5.3 Sample Preparation -- 12.6 Conclusion -- References -- Chapter 13: Co-cultivation of Caco-2 and HT-29MTX -- 13.1 Origin, Features and Mechanisms -- 13.2 Stability/Consistency and Reproducibility -- 13.3 Relevance to the Human In Vivo Situation -- 13.4 General Protocol -- 13.5 Assess Viability -- 13.6 Experimental Readout -- 13.7 Advantages, Disadvantages and Limitations -- 13.8 Conclusions -- References -- Part III: Innate and Adaptive Immune Cells: General Introduction Iván López-Expósito -- Monocytes and Macrophages -- Dendritic Cells -- Human Peripheral Blood Mononuclear Cells. T Lymphocytes or T-Cells -- References -- Chapter 14: THP-1 and U937 Cells -- 14.1 Origin and Some Features of THP-1 and U937 Cells -- 14.2 Stability, Consistency and Reproducibility of the System -- 14.3 Relevance to Human In Vivo Situation -- 14.4 Other Models with the Same Applicability -- 14.5 General Protocol of Culturing THP-1 Cells -- 14.6 Differentiation of THP-1 and U937 Monocytes into Macrophages -- 14.7 Differentiation of THP-1 and U937 Monocytes into Dendritic Cells -- 14.8 Controls to Test Viability and Performance of the Model -- 14.9 Critical Notes -- 14.10 Read-Out of the System -- References -- Chapter 15: Peripheral Blood Mononuclear Cells -- 15.1 Origin -- 15.2 Features and Mechanisms -- 15.3 Stability, Consistency and Reproducibility -- 15.4 Relevance to Human In Vivo Situation -- 15.5 General Protocol -- 15.5.1 Study of Proliferative/Cytotoxic Activity -- 15.5.2 Study of Inflammatory Responses -- 15.6 Assess Viability -- 15.7 Experimental Read Out -- 15.8 Advantages, Disadvantages and Limitations of the System -- 15.9 Conclusions -- References -- Chapter 16: PBMC-Derived T Cells -- 16.1 Introduction and Origin -- 16.2 Features and Mechanisms -- 16.3 Applications of T Cell Cultures -- 16.4 General Protocol -- 16.4.1 T Cell Isolation Protocols -- 16.4.2 Indirect Positive Isolation of Human CD4+ T -- 16.4.2.1 Preparation of Cells and Antibodies -- 16.4.2.2 Coating of PBMCs with CD4 Antibody -- 16.4.2.3 Magnetic Beads Washing Procedure -- 16.4.2.4 Separation of T Cells -- 16.4.2.5 Detachment of T Cells from Beads -- 16.5 Assess Viability -- 16.6 Samples -- 16.7 Experimental Readouts -- References -- Chapter 17: Dendritic Cells -- 17.1 Origin -- 17.2 Features and Mechanisms -- 17.2.1 DC Subsets -- 17.2.1.1 Blood DCs -- 17.2.1.2 Mucosal DCs -- 17.2.1.3 Monocyte-Derived DCs -- 17.3 General Protocols -- 17.3.1 DC Cell Lines. 17.3.2 Isolating Primary DCs from Blood -- 17.3.3 CD34+-Derived DCs -- 17.3.4 Monocyte-Derived DCs -- 17.4 Asses Viability -- 17.5 Experimental Readout -- 17.5.1 Co-stimulation -- 17.5.2 Cytokine Production -- 17.5.3 Other DC Readouts -- 17.6 In Vitro Studies on Food Bioactives Using DCs (Table 17.3) -- 17.7 Critical Notes -- References -- Chapter 18: Co-culture Caco-2/Immune Cells -- 18.1 Origin, Features and Mechanisms -- 18.2 Relevance to Human In Vivo Situation -- 18.2.1 Co-culture Caco-2 and Dendritic Cells -- 18.2.2 Co-culture Caco-2 and B-cells (Raji) -- 18.3 Stability, Consistency and Reproducibility -- 18.4 General Protocol -- 18.4.1 Co-culture of Caco-2/Human Monocyte Derived DCs (Include Contact Dependent Events) -- 18.4.2 Caco-2/Human Monocyte Derived DCs (Soluble Factors) -- 18.4.3 Caco-2/THP-1 (Soluble Factors) -- 18.4.4 Caco-2/PBMCs (Soluble Factors) -- 18.4.5 Caco-2/B Cells -- 18.5 Assess Viability -- 18.6 Experimental Readout -- 18.7 Advantages, Disadvantages and Limitations -- 18.8 Conclusions -- References -- Part IV: Enteroendocrine Cell Models: General Introduction -- References -- Chapter 19: STC-1 Cells -- 19.1 Origin -- 19.2 Features and Mechanisms -- 19.3 Stability, Consistency and Reproducibility -- 19.4 Relevance to Human In Vivo Situation -- 19.5 General Protocol -- 19.5.1 Cell Maintenance Protocol -- 19.5.2 Experimental Protocol for Test Compounds -- 19.6 Assess Viability -- 19.7 Experimental Read out -- 19.8 Conclusions -- References -- Chapter 20: NCI-H716 Cells -- 20.1 Introduction -- 20.2 Origin -- 20.3 Features and Mechanisms -- 20.4 Stability/Consistency/Reproducibility -- 20.5 Relevance to the Human L-Cell In Vivo -- 20.6 General Protocol -- 20.6.1 Cell Maintenance Protocol -- 20.6.2 Experimental Protocol for Test Compounds -- 20.7 Assess Viability -- 20.8 Experimental Readout -- 20.9 Conclusions -- References. Chapter 21: Murine GLUTag Cells. |
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9783319161044 9783319157917 |
callnumber-first |
Q - Science |
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Electronic books. |
genre_facet |
Electronic books. |
url |
https://ebookcentral.proquest.com/lib/oeawat/detail.action?docID=6422901 |
illustrated |
Not Illustrated |
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1231608706 |
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<?xml version="1.0" encoding="UTF-8"?><collection xmlns="http://www.loc.gov/MARC21/slim"><record><leader>12029nam a22005293i 4500</leader><controlfield tag="001">5006422901</controlfield><controlfield tag="003">MiAaPQ</controlfield><controlfield tag="005">20240229073838.0</controlfield><controlfield tag="006">m o d | </controlfield><controlfield tag="007">cr cnu||||||||</controlfield><controlfield tag="008">240229s2015 xx o ||||0 eng d</controlfield><datafield tag="020" ind1=" " ind2=" "><subfield code="a">9783319161044</subfield><subfield code="q">(electronic bk.)</subfield></datafield><datafield tag="020" ind1=" " ind2=" "><subfield code="z">9783319157917</subfield></datafield><datafield tag="035" ind1=" " ind2=" "><subfield code="a">(MiAaPQ)5006422901</subfield></datafield><datafield tag="035" ind1=" " ind2=" "><subfield code="a">(Au-PeEL)EBL6422901</subfield></datafield><datafield tag="035" ind1=" " ind2=" "><subfield code="a">(OCoLC)1231608706</subfield></datafield><datafield tag="040" ind1=" " ind2=" "><subfield code="a">MiAaPQ</subfield><subfield code="b">eng</subfield><subfield code="e">rda</subfield><subfield code="e">pn</subfield><subfield code="c">MiAaPQ</subfield><subfield code="d">MiAaPQ</subfield></datafield><datafield tag="050" ind1=" " ind2="4"><subfield code="a">QH301-705</subfield></datafield><datafield tag="100" ind1="1" ind2=" "><subfield code="a">Verhoeckx, Kitty.</subfield></datafield><datafield tag="245" ind1="1" ind2="4"><subfield code="a">The Impact of Food Bioactives on Health :</subfield><subfield code="b">In Vitro and Ex Vivo Models.</subfield></datafield><datafield tag="250" ind1=" " ind2=" "><subfield code="a">1st ed.</subfield></datafield><datafield tag="264" ind1=" " ind2="1"><subfield code="a">Cham :</subfield><subfield code="b">Springer International Publishing AG,</subfield><subfield code="c">2015.</subfield></datafield><datafield tag="264" ind1=" " ind2="4"><subfield code="c">©2015.</subfield></datafield><datafield tag="300" ind1=" " ind2=" "><subfield code="a">1 online resource (341 pages)</subfield></datafield><datafield tag="336" ind1=" " ind2=" "><subfield code="a">text</subfield><subfield code="b">txt</subfield><subfield code="2">rdacontent</subfield></datafield><datafield tag="337" ind1=" " ind2=" "><subfield code="a">computer</subfield><subfield code="b">c</subfield><subfield code="2">rdamedia</subfield></datafield><datafield tag="338" ind1=" " ind2=" "><subfield code="a">online resource</subfield><subfield code="b">cr</subfield><subfield code="2">rdacarrier</subfield></datafield><datafield tag="505" ind1="0" ind2=" "><subfield code="a">Intro -- Preface -- General Introduction -- Digestion and Absorption -- Cells Present in the Intestine -- Role of Microbiota -- Contents -- Part I: Gastrointestinal Digestion Models, General Introduction -- General Introduction -- References -- Chapter 1: Static Digestion Models: General Introduction -- 1.1 Definition of Concepts: Bioavailability, Bioaccessibility and Bioactivity -- 1.2 Static Methods -- 1.2.1 Solubility/Dialyzability -- 1.2.2 Digestion Conditions -- 1.3 Applications: Advantages and Disadvantages -- 1.4 Static Versus In Vivo Digestion: Conclusions -- References -- Chapter 2: InfoGest Consensus Method -- 2.1 Introduction -- 2.2 The Oral Phase -- 2.3 The Gastric Phase -- 2.4 The Small Intestinal Phase -- 2.5 Practicalities -- 2.6 Sampling -- 2.7 Conclusions -- References -- Chapter 3: Approaches to Static Digestion Models -- 3.1 Introduction -- 3.2 Static Models for Protein Hydrolysis -- 3.3 Static Models for Lipid Hydrolysis -- 3.4 Other Static Models -- References -- Chapter 4: Dynamic Digestion Models: General Introduction -- 4.1 Geometry -- 4.2 Physical Forces -- 4.3 Biochemistry -- References -- Chapter 5: The TNO Gastro-Intestinal Model (TIM) -- 5.1 Introduction -- 5.2 Concept of TIM -- 5.3 TIM-1 -- 5.4 TinyTIM -- 5.5 Advanced Gastric Compartment (TIM-agc) -- 5.6 The Use of TIM to Study the Bio-accessibility of Nutrients -- 5.7 Protein Quality -- 5.8 Prediction of Glycemic Response -- 5.9 Lipids -- 5.10 Conclusions -- References -- Chapter 6: Dynamic Gastric Model (DGM) -- 6.1 Origins and Design of the DGM -- 6.2 General Protocol for DGM Experiments -- 6.3 Uses of the DGM -- 6.3.1 Food-Based Research -- 6.3.2 Pharmaceutical-Based Research -- 6.4 Advantages, Disadvantages and Limitations -- 6.5 Availability of the System -- References -- Chapter 7: Human Gastric Simulator (Riddet Model) -- 7.1 Origins of the HGS.</subfield></datafield><datafield tag="505" ind1="8" ind2=" "><subfield code="a">7.2 Model Description -- 7.2.1 Gastric Compartment -- 7.2.2 Gastric Motility -- 7.2.3 Gastric Emptying -- 7.2.4 Gastric Secretions -- 7.2.5 Temperature Control -- 7.3 Analysis of HGS Biomechanical Relevance -- 7.4 Operating Protocol -- 7.4.1 Preparation of a Food Bolus -- 7.4.2 Gastric Processing -- 7.5 Uses of the HGS -- 7.5.1 Role of ACW Activity on Food Digestion -- 7.5.2 Role of Food Material Properties -- 7.6 Advantages and Limitations -- 7.7 Availability of the System -- References -- Chapter 8: The DIDGI® System -- 8.1 Origins and Design of the DIDGI® System -- 8.2 Validation of DIDGI® for the Digestion of Infant Formula -- 8.2.1 Protocol for the In Vitro Dynamic Digestion of Infant Formula Using the DIDGI® System -- 8.2.2 In Vivo Digestion of Infant Formula on Piglets -- 8.2.3 Comparison In Vitro/In Vivo Data -- 8.3 Advantages, Disadvantages and Limitations -- 8.4 Conclusion and Prospects -- References -- Part II: General Introduction to Cells, Cell Lines and Cell Culture -- Introduction -- Salt Solutions -- Culture Media -- Medium Quality -- pH and CO2 -- Serum Addition -- The Cell Culture Hood -- Cell Culture Terminology (Fig. 1) -- General Cell Culture Protocols -- Trypsinisation and Subculturing of Cells -- Passaging of Cells in Suspension Culture -- Freezing Cells -- The Thawing and Recovery of Cells -- Cell Viability Testing -- Contamination of Cell Cultures -- References -- Chapter 9: Epithelial Cell Models -- General Introduction -- 9.1 Measurement of Transepithelial Electrical Resistance (TEER) -- 9.1.1 Basic Protocol -- 9.1.2 Calculating Transepithelial Resistance -- 9.2 Verification of Monolayer Integrity by Lucifer Yellow Flux -- 9.2.1 Basic Protocol -- 9.3 Summary -- References -- Chapter 10: Caco-2 Cell Line -- 10.1 Origin -- 10.2 Features and Mechanisms -- 10.3 Stability, Consistency and Reproducibility.</subfield></datafield><datafield tag="505" ind1="8" ind2=" "><subfield code="a">10.4 Relevance to Human In Vivo Situation -- 10.5 General Protocols for Caco-2 Cells -- 10.5.1 General Maintenance -- 10.5.2 Protocol for Polarizing Caco-2 Cells in Tissue Culture Inserts -- 10.5.3 Troubleshooting Guide for Transport Experiments Across Caco-2 Monolayers -- 10.6 Applications -- 10.7 Advantages and Disadvantages -- 10.8 Conclusion -- References -- Chapter 11: HT29 Cell Line -- 11.1 Origin -- 11.2 Features and Mechanisms -- 11.3 Stability, Consistency and Reproducibility -- 11.4 Relevance to Human In Vivo Situation -- 11.5 General Protocol for HT29-MTX Cells -- 11.5.1 Cell Maintenance Protocol -- 11.5.2 Experimental Protocol for Test Compounds -- 11.5.2.1 Study of the Mucin-Stimulating Activity -- 11.5.2.2 Evaluation of Transepithelial Absorption by Transwell® Inserts -- 11.6 Experimental Read Out -- 11.6.1 Functionality Studies -- 11.6.2 Transport Studies -- 11.6.3 Microorganisms Survival, Adhesion or Invasion -- 11.7 Conclusions -- References -- Chapter 12: The IPEC-J2 Cell Line -- 12.1 Origin -- 12.2 Special Features/Morphology/Receptors -- 12.3 Stability/Consistency/Reproducibility of the System -- 12.4 Relevance to Human In Vivo Situation -- 12.5 General Protocol -- 12.5.1 Culture Conditions -- 12.5.2 Experimental Readout -- 12.5.3 Sample Preparation -- 12.6 Conclusion -- References -- Chapter 13: Co-cultivation of Caco-2 and HT-29MTX -- 13.1 Origin, Features and Mechanisms -- 13.2 Stability/Consistency and Reproducibility -- 13.3 Relevance to the Human In Vivo Situation -- 13.4 General Protocol -- 13.5 Assess Viability -- 13.6 Experimental Readout -- 13.7 Advantages, Disadvantages and Limitations -- 13.8 Conclusions -- References -- Part III: Innate and Adaptive Immune Cells: General Introduction Iván López-Expósito -- Monocytes and Macrophages -- Dendritic Cells -- Human Peripheral Blood Mononuclear Cells.</subfield></datafield><datafield tag="505" ind1="8" ind2=" "><subfield code="a">T Lymphocytes or T-Cells -- References -- Chapter 14: THP-1 and U937 Cells -- 14.1 Origin and Some Features of THP-1 and U937 Cells -- 14.2 Stability, Consistency and Reproducibility of the System -- 14.3 Relevance to Human In Vivo Situation -- 14.4 Other Models with the Same Applicability -- 14.5 General Protocol of Culturing THP-1 Cells -- 14.6 Differentiation of THP-1 and U937 Monocytes into Macrophages -- 14.7 Differentiation of THP-1 and U937 Monocytes into Dendritic Cells -- 14.8 Controls to Test Viability and Performance of the Model -- 14.9 Critical Notes -- 14.10 Read-Out of the System -- References -- Chapter 15: Peripheral Blood Mononuclear Cells -- 15.1 Origin -- 15.2 Features and Mechanisms -- 15.3 Stability, Consistency and Reproducibility -- 15.4 Relevance to Human In Vivo Situation -- 15.5 General Protocol -- 15.5.1 Study of Proliferative/Cytotoxic Activity -- 15.5.2 Study of Inflammatory Responses -- 15.6 Assess Viability -- 15.7 Experimental Read Out -- 15.8 Advantages, Disadvantages and Limitations of the System -- 15.9 Conclusions -- References -- Chapter 16: PBMC-Derived T Cells -- 16.1 Introduction and Origin -- 16.2 Features and Mechanisms -- 16.3 Applications of T Cell Cultures -- 16.4 General Protocol -- 16.4.1 T Cell Isolation Protocols -- 16.4.2 Indirect Positive Isolation of Human CD4+ T -- 16.4.2.1 Preparation of Cells and Antibodies -- 16.4.2.2 Coating of PBMCs with CD4 Antibody -- 16.4.2.3 Magnetic Beads Washing Procedure -- 16.4.2.4 Separation of T Cells -- 16.4.2.5 Detachment of T Cells from Beads -- 16.5 Assess Viability -- 16.6 Samples -- 16.7 Experimental Readouts -- References -- Chapter 17: Dendritic Cells -- 17.1 Origin -- 17.2 Features and Mechanisms -- 17.2.1 DC Subsets -- 17.2.1.1 Blood DCs -- 17.2.1.2 Mucosal DCs -- 17.2.1.3 Monocyte-Derived DCs -- 17.3 General Protocols -- 17.3.1 DC Cell Lines.</subfield></datafield><datafield tag="505" ind1="8" ind2=" "><subfield code="a">17.3.2 Isolating Primary DCs from Blood -- 17.3.3 CD34+-Derived DCs -- 17.3.4 Monocyte-Derived DCs -- 17.4 Asses Viability -- 17.5 Experimental Readout -- 17.5.1 Co-stimulation -- 17.5.2 Cytokine Production -- 17.5.3 Other DC Readouts -- 17.6 In Vitro Studies on Food Bioactives Using DCs (Table 17.3) -- 17.7 Critical Notes -- References -- Chapter 18: Co-culture Caco-2/Immune Cells -- 18.1 Origin, Features and Mechanisms -- 18.2 Relevance to Human In Vivo Situation -- 18.2.1 Co-culture Caco-2 and Dendritic Cells -- 18.2.2 Co-culture Caco-2 and B-cells (Raji) -- 18.3 Stability, Consistency and Reproducibility -- 18.4 General Protocol -- 18.4.1 Co-culture of Caco-2/Human Monocyte Derived DCs (Include Contact Dependent Events) -- 18.4.2 Caco-2/Human Monocyte Derived DCs (Soluble Factors) -- 18.4.3 Caco-2/THP-1 (Soluble Factors) -- 18.4.4 Caco-2/PBMCs (Soluble Factors) -- 18.4.5 Caco-2/B Cells -- 18.5 Assess Viability -- 18.6 Experimental Readout -- 18.7 Advantages, Disadvantages and Limitations -- 18.8 Conclusions -- References -- Part IV: Enteroendocrine Cell Models: General Introduction -- References -- Chapter 19: STC-1 Cells -- 19.1 Origin -- 19.2 Features and Mechanisms -- 19.3 Stability, Consistency and Reproducibility -- 19.4 Relevance to Human In Vivo Situation -- 19.5 General Protocol -- 19.5.1 Cell Maintenance Protocol -- 19.5.2 Experimental Protocol for Test Compounds -- 19.6 Assess Viability -- 19.7 Experimental Read out -- 19.8 Conclusions -- References -- Chapter 20: NCI-H716 Cells -- 20.1 Introduction -- 20.2 Origin -- 20.3 Features and Mechanisms -- 20.4 Stability/Consistency/Reproducibility -- 20.5 Relevance to the Human L-Cell In Vivo -- 20.6 General Protocol -- 20.6.1 Cell Maintenance Protocol -- 20.6.2 Experimental Protocol for Test Compounds -- 20.7 Assess Viability -- 20.8 Experimental Readout -- 20.9 Conclusions -- References.</subfield></datafield><datafield tag="505" ind1="8" ind2=" "><subfield code="a">Chapter 21: Murine GLUTag Cells.</subfield></datafield><datafield tag="588" ind1=" " ind2=" "><subfield code="a">Description based on publisher supplied metadata and other sources.</subfield></datafield><datafield tag="590" ind1=" " ind2=" "><subfield code="a">Electronic reproduction. Ann Arbor, Michigan : ProQuest Ebook Central, 2024. Available via World Wide Web. 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