Protocols for Pre-Field Screening of Mutants for Salt Tolerance in Rice, Wheat and Barley.

• Author: Bado, Souleymane.
• TeilnehmendeR: Forster, Brian P.; Ghanim, Abdelbagi.; Jankowicz-Cieslak, Joanna.; Günter, Berthold.; Luxiang, Liu.
• Place / Publishing House: Cham : : Springer International Publishing AG,, 2016.; ©2016.
• Year of Publication: 2016
• Edition: 1st ed.
• Language: English
• Physical Description: 1 online resource (44 pages)

Table of Contents

• Intro
• Preface
• Acknowledgements
• Contents
• Chapter 1: Introduction
• 1.1 Background
• 1.2 Biology of Salt Tolerance
• 1.3 Screening Methods
• 1.4 Breeding for Salt Tolerance
• 1.4.1 Traditional Breeding
• 1.4.2 Induced Mutation in Breeding for Salt Tolerance
• 1.5 Need for Reliable Screening Techniques for Pre-field Selection
• References
• Chapter 2: Objectives
• 2.1 Monitoring Field Salinity
• 2.2 Screening for Salt Tolerance
• 2.3 Benefits and Drawbacks of Seedling Screening
• References
• Chapter 3: Protocol for Measuring Soil Salinity
• 3.1 Background
• 3.2 Instruments and Reagents
• 3.3 Preparation of 5:1 Water/Soil Extract
• 3.4 Preparation of 1:1 Water/Soil Extract
• 3.5 Important Considerations
• 3.6 Measurements
• 3.6.1 Weight Method
• 3.6.1.1 Instruments and Reagents
• 3.6.1.2 Method
• 3.6.1.3 Calculation of Total Water-Soluble Salts
• 3.6.1.4 Important Considerations
• 3.6.2 Conductivity Method
• 3.6.2.1 Instruments
• 3.6.2.2 Method
• 3.6.2.3 Calculation
• 3.6.2.4 Important Considerations
• Chapter 4: Protocol for Screening for Salt Tolerance in Rice
• 4.1 Introduction
• 4.2 Equipment
• 4.3 Plant Materials
• 4.4 Setting Up Hydroponic Hardware
• 4.5 Preparation of Hydroponic Solutions
• 4.6 Seed Storage and Seed Pregermination Treatments
• 4.7 Seedling Establishment in Hydroponics
• 4.8 Care of Plants in Hydroponics
• 4.9 Salt Treatment
• 4.10 Scoring
• 4.11 Recovery of Salt-Tolerant Lines
• 4.12 Examples
• References
• Chapter 5: Protocol for Screening for Salt Tolerance in Barley and Wheat
• 5.1 Introduction
• 5.2 Adaptations of Rice Protocol to Wheat and Barley
• 5.2.1 Germination
• 5.2.2 Hydroponic Solution
• 5.2.3 Aeration
• 5.2.4 Glasshouse Conditions
• 5.2.5 Test Salt Concentrations
• Reference.