Protocols for Pre-Field Screening of Mutants for Salt Tolerance in Rice, Wheat and Barley.

• Author: Bado, Souleymane.
• TeilnehmendeR: Forster, Brian P.; Ghanim, Abdelbagi.; Jankowicz-Cieslak, Joanna.; Günter, Berthold.; Luxiang, Liu.
• Place / Publishing House: Cham : : Springer International Publishing AG,, 2016.; ©2016.
• Year of Publication: 2016
• Edition: 1st ed.
• Language: English
• Physical Description: 1 online resource (44 pages)

Table of Contents

Intro -- Preface -- Acknowledgements -- Contents -- Chapter 1: Introduction -- 1.1 Background -- 1.2 Biology of Salt Tolerance -- 1.3 Screening Methods -- 1.4 Breeding for Salt Tolerance -- 1.4.1 Traditional Breeding -- 1.4.2 Induced Mutation in Breeding for Salt Tolerance -- 1.5 Need for Reliable Screening Techniques for Pre-field Selection -- References -- Chapter 2: Objectives -- 2.1 Monitoring Field Salinity -- 2.2 Screening for Salt Tolerance -- 2.3 Benefits and Drawbacks of Seedling Screening -- References -- Chapter 3: Protocol for Measuring Soil Salinity -- 3.1 Background -- 3.2 Instruments and Reagents -- 3.3 Preparation of 5:1 Water/Soil Extract -- 3.4 Preparation of 1:1 Water/Soil Extract -- 3.5 Important Considerations -- 3.6 Measurements -- 3.6.1 Weight Method -- 3.6.1.1 Instruments and Reagents -- 3.6.1.2 Method -- 3.6.1.3 Calculation of Total Water-Soluble Salts -- 3.6.1.4 Important Considerations -- 3.6.2 Conductivity Method -- 3.6.2.1 Instruments -- 3.6.2.2 Method -- 3.6.2.3 Calculation -- 3.6.2.4 Important Considerations -- Chapter 4: Protocol for Screening for Salt Tolerance in Rice -- 4.1 Introduction -- 4.2 Equipment -- 4.3 Plant Materials -- 4.4 Setting Up Hydroponic Hardware -- 4.5 Preparation of Hydroponic Solutions -- 4.6 Seed Storage and Seed Pregermination Treatments -- 4.7 Seedling Establishment in Hydroponics -- 4.8 Care of Plants in Hydroponics -- 4.9 Salt Treatment -- 4.10 Scoring -- 4.11 Recovery of Salt-Tolerant Lines -- 4.12 Examples -- References -- Chapter 5: Protocol for Screening for Salt Tolerance in Barley and Wheat -- 5.1 Introduction -- 5.2 Adaptations of Rice Protocol to Wheat and Barley -- 5.2.1 Germination -- 5.2.2 Hydroponic Solution -- 5.2.3 Aeration -- 5.2.4 Glasshouse Conditions -- 5.2.5 Test Salt Concentrations -- Reference.