Low-Cost Methods for Molecular Characterization of Mutant Plants : : Tissue Desiccation, DNA Extraction and Mutation Discovery: Protocols.

• Author: Till, Bradley J.
• TeilnehmendeR: Jankowicz-Cieslak, Joanna.; Huynh, Owen A.; Beshir, Mayada M.; Laport, Robert G.; Hofinger, Bernhard J.
• Place / Publishing House: Cham : : Springer International Publishing AG,, 2015.; ©2015.
• Year of Publication: 2015
• Edition: 1st ed.
• Language: English
• Physical Description: 1 online resource (42 pages)

Table of Contents

Intro -- Foreword -- Acknowledgements -- Contents -- Chapter 1: Introduction -- 1.1 Background -- 1.2 Methods Used to Isolate Genomic DNA from Plant Tissues -- 1.3 Methods for the Discovery and Characterization of Induced and Natural Nucleotide Variation in Plant Genomes -- References -- Chapter 2: Health and Safety Considerations -- 2.1 Guidelines -- 2.2 Preparation of a Home-Made Chemical Spill Kit -- Reference -- Chapter 3: Sample Collection and Storage -- 3.1 Background -- 3.2 Materials -- 3.3 Methods -- References -- Chapter 4: Low-Cost DNA Extraction -- 4.1 Materials -- 4.2 Methods -- 4.2.1 Preparation of Silica Powder DNA Binding Solution -- 4.2.2 Low-Cost Extraction of Genomic DNA -- 4.3 Alternative Buffers for DNA Extraction -- Reference -- Chapter 5: PCR Amplification for Low-Cost Mutation Discovery -- 5.1 Materials -- 5.2 Methods -- References -- Chapter 6: Enzymatic Mismatch Cleavage and Agarose Gel Evaluation of Samples -- 6.1 Materials -- 6.2 Methods -- Reference -- Chapter 7: Alternative Enzymology for Mismatch Cleavage for TILLING and Ecotilling: Extraction of Enzymes from Common Weedy Pl... -- 7.1 Materials -- 7.2 Methods -- 7.2.1 Enzyme Extraction -- 7.2.2 Concentration of Enzymes Using Amicon Ultra 10 kDa MWCO Centrifugal Filter Devices (for 0.5 ml Starting Volume -- in 1.5-m... -- 7.2.3 Test of Mismatch Cleavage Activity -- Reference -- Chapter 8: Example Data -- 8.1 Quality of Genomic DNA Obtained by Silica Powder-Based DNA Extraction Method -- 8.2 Quality of Genomic DNA Obtained by Silica Powder-Based DNA Extraction Method Using Alternative Buffers -- 8.2.1 Summary -- 8.3 Example of PCR Products Using TILLING Primers with Source Genomic DNA from a Commercial Kit and Low-Cost Silica Method -- 8.4 Example of Low-Cost Agarose Gel-Based TILLING Assays for the Discovery of Induced Point Mutations.

8.5 Example of Enzyme Activity Recovered from Weeds Compared to Crude Celery Juice Extract -- 8.5.1 Summary -- References -- Chapter 9: Conclusions.