Low-Cost Methods for Molecular Characterization of Mutant Plants : : Tissue Desiccation, DNA Extraction and Mutation Discovery: Protocols.
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Place / Publishing House: | Cham : : Springer International Publishing AG,, 2015. ©2015. |
Year of Publication: | 2015 |
Edition: | 1st ed. |
Language: | English |
Online Access: | |
Physical Description: | 1 online resource (42 pages) |
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001 | 5006422689 | ||
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008 | 240229s2015 xx o ||||0 eng d | ||
020 | |a 9783319162591 |q (electronic bk.) | ||
020 | |z 9783319162584 | ||
035 | |a (MiAaPQ)5006422689 | ||
035 | |a (Au-PeEL)EBL6422689 | ||
035 | |a (OCoLC)1231605307 | ||
040 | |a MiAaPQ |b eng |e rda |e pn |c MiAaPQ |d MiAaPQ | ||
050 | 4 | |a TP248.27.P55 | |
100 | 1 | |a Till, Bradley J. | |
245 | 1 | 0 | |a Low-Cost Methods for Molecular Characterization of Mutant Plants : |b Tissue Desiccation, DNA Extraction and Mutation Discovery: Protocols. |
250 | |a 1st ed. | ||
264 | 1 | |a Cham : |b Springer International Publishing AG, |c 2015. | |
264 | 4 | |c ©2015. | |
300 | |a 1 online resource (42 pages) | ||
336 | |a text |b txt |2 rdacontent | ||
337 | |a computer |b c |2 rdamedia | ||
338 | |a online resource |b cr |2 rdacarrier | ||
505 | 0 | |a Intro -- Foreword -- Acknowledgements -- Contents -- Chapter 1: Introduction -- 1.1 Background -- 1.2 Methods Used to Isolate Genomic DNA from Plant Tissues -- 1.3 Methods for the Discovery and Characterization of Induced and Natural Nucleotide Variation in Plant Genomes -- References -- Chapter 2: Health and Safety Considerations -- 2.1 Guidelines -- 2.2 Preparation of a Home-Made Chemical Spill Kit -- Reference -- Chapter 3: Sample Collection and Storage -- 3.1 Background -- 3.2 Materials -- 3.3 Methods -- References -- Chapter 4: Low-Cost DNA Extraction -- 4.1 Materials -- 4.2 Methods -- 4.2.1 Preparation of Silica Powder DNA Binding Solution -- 4.2.2 Low-Cost Extraction of Genomic DNA -- 4.3 Alternative Buffers for DNA Extraction -- Reference -- Chapter 5: PCR Amplification for Low-Cost Mutation Discovery -- 5.1 Materials -- 5.2 Methods -- References -- Chapter 6: Enzymatic Mismatch Cleavage and Agarose Gel Evaluation of Samples -- 6.1 Materials -- 6.2 Methods -- Reference -- Chapter 7: Alternative Enzymology for Mismatch Cleavage for TILLING and Ecotilling: Extraction of Enzymes from Common Weedy Pl... -- 7.1 Materials -- 7.2 Methods -- 7.2.1 Enzyme Extraction -- 7.2.2 Concentration of Enzymes Using Amicon Ultra 10 kDa MWCO Centrifugal Filter Devices (for 0.5 ml Starting Volume -- in 1.5-m... -- 7.2.3 Test of Mismatch Cleavage Activity -- Reference -- Chapter 8: Example Data -- 8.1 Quality of Genomic DNA Obtained by Silica Powder-Based DNA Extraction Method -- 8.2 Quality of Genomic DNA Obtained by Silica Powder-Based DNA Extraction Method Using Alternative Buffers -- 8.2.1 Summary -- 8.3 Example of PCR Products Using TILLING Primers with Source Genomic DNA from a Commercial Kit and Low-Cost Silica Method -- 8.4 Example of Low-Cost Agarose Gel-Based TILLING Assays for the Discovery of Induced Point Mutations. | |
505 | 8 | |a 8.5 Example of Enzyme Activity Recovered from Weeds Compared to Crude Celery Juice Extract -- 8.5.1 Summary -- References -- Chapter 9: Conclusions. | |
588 | |a Description based on publisher supplied metadata and other sources. | ||
590 | |a Electronic reproduction. Ann Arbor, Michigan : ProQuest Ebook Central, 2024. Available via World Wide Web. Access may be limited to ProQuest Ebook Central affiliated libraries. | ||
655 | 4 | |a Electronic books. | |
700 | 1 | |a Jankowicz-Cieslak, Joanna. | |
700 | 1 | |a Huynh, Owen A. | |
700 | 1 | |a Beshir, Mayada M. | |
700 | 1 | |a Laport, Robert G. | |
700 | 1 | |a Hofinger, Bernhard J. | |
776 | 0 | 8 | |i Print version: |a Till, Bradley J. |t Low-Cost Methods for Molecular Characterization of Mutant Plants |d Cham : Springer International Publishing AG,c2015 |z 9783319162584 |
797 | 2 | |a ProQuest (Firm) | |
856 | 4 | 0 | |u https://ebookcentral.proquest.com/lib/oeawat/detail.action?docID=6422689 |z Click to View |