Application of diffractive lens arrays in confocal microscopy / / Zheng Li.
Diffractive lens arrays are proposed in this work for application in reflected-light confocal microscopes. They have overcome the limitations between fields of view and resolution of traditional objectives. Experiments of multi-spot confocal imaging in surface metrology and fluorescence microscopy h...
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Place / Publishing House: | Karlsruhe : : KIT Scientific Publishing,, [2022] ©2022 |
Year of Publication: | 2022 |
Language: | English |
Series: | Schriftenreihe automatische Sichtprüfung und Bildverarbeitung
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Physical Description: | 1 online resource (xv, 148 pages) :; illustrations |
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Li, Zheng, author. Application of diffractive lens arrays in confocal microscopy / Zheng Li. Karlsruhe : KIT Scientific Publishing, [2022] ©2022 1 online resource (xv, 148 pages) : illustrations text txt rdacontent computer c rdamedia online resource cr rdacarrier Schriftenreihe automatische Sichtprüfung und Bildverarbeitung Description based on: online resource; title from PDF information screen (Worldcat, viewed March 30, 2023). Diffractive lens arrays are proposed in this work for application in reflected-light confocal microscopes. They have overcome the limitations between fields of view and resolution of traditional objectives. Experiments of multi-spot confocal imaging in surface metrology and fluorescence microscopy have been demonstrated based on the proposed concepts, which have shown capabilities of high-resolution measurement over a large area. Abstract -- Kurzfassung -- Acknowledgements -- Notation -- Acronyms -- 1 Introduction -- 1.1 Motivation -- 1.2 Related work -- 1.3 Main contributions -- 1.4 Thesis outline -- 2 Theoretical Background -- 2.1 Diffraction simulation -- 2.1.1 Maxwell's equations -- 2.1.2 Scalar approximation and solutions -- 2.1.3 Numerical implementation -- 2.2 Theory of confocal microscopy -- 2.2.1 Focusing by a thin lens -- 2.2.2 PSF of a thin lens -- 2.2.3 PSF of a confocal scanning microscope -- 3 Design and Simulation -- 3.1 See-through DLA design -- 3.1.1 System overview -- 3.1.2 Design process -- 3.1.3 Simulation and optimization -- 3.2 Direct-imaging DLA design -- 3.2.1 System overview -- 3.2.2 Design process -- 3.2.3 Simulation and optimization -- 4 Experiment Results -- 4.1 Spot characterization -- 4.1.1 Spot measurement of See-through DLAs -- 4.1.2 Spot measurement of Direct-imaging DLAs -- 4.2 Lateral measurement -- 4.2.1 Lateral measurement by See-through DLAs -- 4.2.2 Lateral measurement by Direct-imaging DLAs -- 4.3 Axial measurement -- 4.3.1 Axial response of See-through DLAs -- 4.3.2 Axial response of Direct-imaging DLAs -- 4.3.3 Interference measurement by See-through DLAs -- 4.3.4 Measurement of a step height target -- 4.4 Fluorescence measurement -- 5 Conclusions -- 5.1 Summary of the work -- 5.2 Outlook -- Bibliography -- Publications -- Appendix -- A Kirchhoff's Diffraction Formulation. Confocal microscopy. Fluorescence microscopy. 1000145608 |
language |
English |
format |
eBook |
author |
Li, Zheng, |
spellingShingle |
Li, Zheng, Application of diffractive lens arrays in confocal microscopy / Schriftenreihe automatische Sichtprüfung und Bildverarbeitung Abstract -- Kurzfassung -- Acknowledgements -- Notation -- Acronyms -- 1 Introduction -- 1.1 Motivation -- 1.2 Related work -- 1.3 Main contributions -- 1.4 Thesis outline -- 2 Theoretical Background -- 2.1 Diffraction simulation -- 2.1.1 Maxwell's equations -- 2.1.2 Scalar approximation and solutions -- 2.1.3 Numerical implementation -- 2.2 Theory of confocal microscopy -- 2.2.1 Focusing by a thin lens -- 2.2.2 PSF of a thin lens -- 2.2.3 PSF of a confocal scanning microscope -- 3 Design and Simulation -- 3.1 See-through DLA design -- 3.1.1 System overview -- 3.1.2 Design process -- 3.1.3 Simulation and optimization -- 3.2 Direct-imaging DLA design -- 3.2.1 System overview -- 3.2.2 Design process -- 3.2.3 Simulation and optimization -- 4 Experiment Results -- 4.1 Spot characterization -- 4.1.1 Spot measurement of See-through DLAs -- 4.1.2 Spot measurement of Direct-imaging DLAs -- 4.2 Lateral measurement -- 4.2.1 Lateral measurement by See-through DLAs -- 4.2.2 Lateral measurement by Direct-imaging DLAs -- 4.3 Axial measurement -- 4.3.1 Axial response of See-through DLAs -- 4.3.2 Axial response of Direct-imaging DLAs -- 4.3.3 Interference measurement by See-through DLAs -- 4.3.4 Measurement of a step height target -- 4.4 Fluorescence measurement -- 5 Conclusions -- 5.1 Summary of the work -- 5.2 Outlook -- Bibliography -- Publications -- Appendix -- A Kirchhoff's Diffraction Formulation. |
author_facet |
Li, Zheng, |
author_variant |
z l zl |
author_role |
VerfasserIn |
author_sort |
Li, Zheng, |
title |
Application of diffractive lens arrays in confocal microscopy / |
title_full |
Application of diffractive lens arrays in confocal microscopy / Zheng Li. |
title_fullStr |
Application of diffractive lens arrays in confocal microscopy / Zheng Li. |
title_full_unstemmed |
Application of diffractive lens arrays in confocal microscopy / Zheng Li. |
title_auth |
Application of diffractive lens arrays in confocal microscopy / |
title_new |
Application of diffractive lens arrays in confocal microscopy / |
title_sort |
application of diffractive lens arrays in confocal microscopy / |
series |
Schriftenreihe automatische Sichtprüfung und Bildverarbeitung |
series2 |
Schriftenreihe automatische Sichtprüfung und Bildverarbeitung |
publisher |
KIT Scientific Publishing, |
publishDate |
2022 |
physical |
1 online resource (xv, 148 pages) : illustrations |
contents |
Abstract -- Kurzfassung -- Acknowledgements -- Notation -- Acronyms -- 1 Introduction -- 1.1 Motivation -- 1.2 Related work -- 1.3 Main contributions -- 1.4 Thesis outline -- 2 Theoretical Background -- 2.1 Diffraction simulation -- 2.1.1 Maxwell's equations -- 2.1.2 Scalar approximation and solutions -- 2.1.3 Numerical implementation -- 2.2 Theory of confocal microscopy -- 2.2.1 Focusing by a thin lens -- 2.2.2 PSF of a thin lens -- 2.2.3 PSF of a confocal scanning microscope -- 3 Design and Simulation -- 3.1 See-through DLA design -- 3.1.1 System overview -- 3.1.2 Design process -- 3.1.3 Simulation and optimization -- 3.2 Direct-imaging DLA design -- 3.2.1 System overview -- 3.2.2 Design process -- 3.2.3 Simulation and optimization -- 4 Experiment Results -- 4.1 Spot characterization -- 4.1.1 Spot measurement of See-through DLAs -- 4.1.2 Spot measurement of Direct-imaging DLAs -- 4.2 Lateral measurement -- 4.2.1 Lateral measurement by See-through DLAs -- 4.2.2 Lateral measurement by Direct-imaging DLAs -- 4.3 Axial measurement -- 4.3.1 Axial response of See-through DLAs -- 4.3.2 Axial response of Direct-imaging DLAs -- 4.3.3 Interference measurement by See-through DLAs -- 4.3.4 Measurement of a step height target -- 4.4 Fluorescence measurement -- 5 Conclusions -- 5.1 Summary of the work -- 5.2 Outlook -- Bibliography -- Publications -- Appendix -- A Kirchhoff's Diffraction Formulation. |
isbn |
1000145608 |
callnumber-first |
Q - Science |
callnumber-subject |
QH - Natural History and Biology |
callnumber-label |
QH224 |
callnumber-sort |
QH 3224 L5 42022 |
illustrated |
Illustrated |
dewey-hundreds |
500 - Science |
dewey-tens |
500 - Science |
dewey-ones |
502 - Miscellany |
dewey-full |
502.82 |
dewey-sort |
3502.82 |
dewey-raw |
502.82 |
dewey-search |
502.82 |
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Application of diffractive lens arrays in confocal microscopy / |
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