Whole-Body Regeneration : Methods and Protocols / / edited by Simon Blanchoud, Brigitte Galliot.
This Open Access volume provides a comprehensive overview of the latest tools available to scientists to study the many facets of whole-body regeneration (WBR). The chapters in this book are organized into six parts. Part One provides a historical overview on the study of the WBR phenomena focusing...
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| Superior document: | Methods in Molecular Biology, 2450 |
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| HerausgeberIn: | |
| Place / Publishing House: | New York, NY : : Springer US :, Imprint: Humana,, 2022. |
| Year of Publication: | 2022 |
| Edition: | 1st ed. 2022. |
| Language: | English |
| Series: | Methods in Molecular Biology,
2450 |
| Physical Description: | 1 online resource (XVII, 685 p. 131 illus., 123 illus. in color.) |
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Table of Contents:
- The Hazards of Regeneration: From Morgan’s Legacy to Evo-Devo
- Studying Regeneration in Ascidians: An Historical Overview
- Studying Protista WBR and Repair using Physarum polycephalum
- Studying Porifera WBR using the Calcerous Sponges Leucosolenia
- Studying Ctenophora WBR using Mnemiopsis leidyi
- Studying Placozoa WBR in the Simplest Metazoan Animal, Trichoplax adhaerens
- Collecting and Culturing Kamptozoans for Regenerative Studies
- Collecting and Culturing Bryozoans for Regenerative Studies
- Studying Annelida Regeneration in a Novel Model Organism: The Freshwater Aeolosoma viride
- Studying Annelida Body Regeneration under Environmental Stress in Diopatra neapolitana
- Studying Annelida Regeneration using Platynereis dumerilii
- Collecting and Culturing Lineus sanguineus to Study Nemertea WBR
- Studying Xenacoelomorpha WBR using Isodiametra pulchra
- Studying Echinodermata Arm Explant Regeneration using Echinaster sepositus
- Studying Hemichordata WBR using Ptychodera flava
- Studying Tunicata WBR using Botrylloides anceps
- In Situ Hybridization to Identify Stem Cells in the Freshwater Sponge Ephydatia fluviatilis
- Isolation and Maintenance of In Vitro Cell Cultures from the Ctenophore Mnemiopsis leidyi
- Analysis of Spatial Gene Expression at the Cellular Level in Stony Corals
- Studying Stem Cell Biology in Intact and Whole-Body Regenerating Hydra by Flow Cytometry
- Non-Invasive Intra-Vascular Micro-Transfusion in Colonial Tunicates
- Gene Manipulation in Hydractinia
- Manipulation of Gene Activity in the Regenerative Model Sea Anemone, Nematostella vectensis
- Monitoring Telomere Maintenance during Regeneration of Annelids
- Analysis of DNA Double Stranded Breaks using the COMET Assay in Planarians
- Random Integration Transgenesis in a Free-Living Regenerative Flatworm Macrostomum lignano
- RNAi Screening to Assess Tissue Regeneration in Planarians
- Monitoring Chromatin Regulation in Planarians using Chromatin Immunoprecipitation followed by Sequencing (ChIP-seq)
- Assessing Chromatin Accessibility during WBR in Acoels
- Single Cell Transcriptomic Analysis in the Regenerating Cnidarian Nematostella vectensis
- Characterization of Soluble Cell-Free Coelomic Fluid Proteome from the Starfish Marthasterias glacialis
- Using RNA-Sequencing for Transcriptome Profiling of Botrylloides sp. Regeneration
- Studying Mechanical Oscillations during Whole-Body Regeneration in Hydra
- Combining RNAi-Mediated β-catenin Inhibition and Reaggregation to Study Hydra Whole-Body Regeneration
- Creating a User-Friendly and Open-Access Gene Expression Database for Comparing Embryonic Development and Regeneration in Nematostelle vectensis
- Formalizing Phenotypes of Regeneration.