Whole-Body Regeneration : Methods and Protocols / / edited by Simon Blanchoud, Brigitte Galliot.

• Superior document: Methods in Molecular Biology, 2450
• Author: Bale, Gemma
• HerausgeberIn: Blanchoud, Simon.; Galliot, Brigitte.
• Place / Publishing House: New York, NY : : Springer US :, Imprint: Humana,, 2022.
• Year of Publication: 2022
• Edition: 1st ed. 2022.
• Language: English
• Series: Methods in Molecular Biology, 2450
• Physical Description: 1 online resource (XVII, 685 p. 131 illus., 123 illus. in color.)

This Open Access volume provides a comprehensive overview of the latest tools available to scientists to study the many facets of whole-body regeneration (WBR). The chapters in this book are organized into six parts. Part One provides a historical overview on the study of the WBR phenomena focusing on the primary challenges of this research. Parts Two and Three explore a series of non-vertebrate zoological contexts that provide experimental models for WBR, showing how they can be approached with cellular tools. Parts Four, Five, and Six discuss the future advancements of WBR, reporting about the cutting-edge techniques in genetics and omics used to dissect the underlying mechanisms of WBR, and systems biology approaches to reach a synthetic view of WBR. Written in the highly successful Methods in Molecular Biology series format, chapters include introductions to their respective topics, lists of the necessary materials and reagents, step-by-step, readily reproducible laboratory protocols, and tips on troubleshooting and avoiding known pitfalls. Authoritative and thorough, Whole-Body Regeneration: Methods and Protocols is a valuable resource for scientists and researchers who want to learn more about this important and developing field.

Table of Contents

The Hazards of Regeneration: From Morgan’s Legacy to Evo-Devo -- Studying Regeneration in Ascidians: An Historical Overview -- Studying Protista WBR and Repair using Physarum polycephalum -- Studying Porifera WBR using the Calcerous Sponges Leucosolenia -- Studying Ctenophora WBR using Mnemiopsis leidyi -- Studying Placozoa WBR in the Simplest Metazoan Animal, Trichoplax adhaerens -- Collecting and Culturing Kamptozoans for Regenerative Studies -- Collecting and Culturing Bryozoans for Regenerative Studies -- Studying Annelida Regeneration in a Novel Model Organism: The Freshwater Aeolosoma viride -- Studying Annelida Body Regeneration under Environmental Stress in Diopatra neapolitana -- Studying Annelida Regeneration using Platynereis dumerilii -- Collecting and Culturing Lineus sanguineus to Study Nemertea WBR -- Studying Xenacoelomorpha WBR using Isodiametra pulchra -- Studying Echinodermata Arm Explant Regeneration using Echinaster sepositus -- Studying Hemichordata WBR using Ptychodera flava -- Studying Tunicata WBR using Botrylloides anceps -- In Situ Hybridization to Identify Stem Cells in the Freshwater Sponge Ephydatia fluviatilis -- Isolation and Maintenance of In Vitro Cell Cultures from the Ctenophore Mnemiopsis leidyi -- Analysis of Spatial Gene Expression at the Cellular Level in Stony Corals -- Studying Stem Cell Biology in Intact and Whole-Body Regenerating Hydra by Flow Cytometry -- Non-Invasive Intra-Vascular Micro-Transfusion in Colonial Tunicates -- Gene Manipulation in Hydractinia -- Manipulation of Gene Activity in the Regenerative Model Sea Anemone, Nematostella vectensis -- Monitoring Telomere Maintenance during Regeneration of Annelids -- Analysis of DNA Double Stranded Breaks using the COMET Assay in Planarians -- Random Integration Transgenesis in a Free-Living Regenerative Flatworm Macrostomum lignano -- RNAi Screening to Assess Tissue Regeneration in Planarians -- Monitoring Chromatin Regulation in Planarians using Chromatin Immunoprecipitation followed by Sequencing (ChIP-seq) -- Assessing Chromatin Accessibility during WBR in Acoels -- Single Cell Transcriptomic Analysis in the Regenerating Cnidarian Nematostella vectensis -- Characterization of Soluble Cell-Free Coelomic Fluid Proteome from the Starfish Marthasterias glacialis -- Using RNA-Sequencing for Transcriptome Profiling of Botrylloides sp. Regeneration -- Studying Mechanical Oscillations during Whole-Body Regeneration in Hydra -- Combining RNAi-Mediated β-catenin Inhibition and Reaggregation to Study Hydra Whole-Body Regeneration -- Creating a User-Friendly and Open-Access Gene Expression Database for Comparing Embryonic Development and Regeneration in Nematostelle vectensis -- Formalizing Phenotypes of Regeneration.