Mutation Breeding in Coffee with Special Reference to Leaf Rust : : Protocols.

• Author: Ingelbrecht, Ivan L. W.
• TeilnehmendeR: Silva, Maria do Céu Lavado da.; Jankowicz-Cieslak, Joanna.
• Place / Publishing House: Berlin, Heidelberg : : Springer Berlin / Heidelberg,, 2023.; ©2023.
• Year of Publication: 2023
• Edition: 1st ed.
• Language: English
• Physical Description: 1 online resource (316 pages)

Table of Contents

• Intro
• Preface
• Acknowledgements
• Contents
• Contributors
• Introduction
• Mutation Breeding in Arabica Coffee
• 1 General Principles of Plant Mutation Breeding
• 2 Breeding Limitations in Arabica Coffee
• 3 Mutation Breeding in Arabica Coffee
• 3.1 Background
• 3.2 The Need for Radiosensitivity Testing
• 3.3 Choice of Material for Mutation Induction
• 3.4 Enabling Biotechnology and Genomics Tools
• References
• Coffee Leaf Rust Resistance: An Overview
• 1 Introduction
• 2 Coffee's Main Diseases
• 3 CLR Causal Agent: Hemileia vastatrix
• 3.1 Life Cycle and Infection Process
• 3.2 Genetic and Physiological Diversity
• 3.3 Implications of Rust Molecular Diversity and Population Dynamics in Coffee Breeding
• 4 Coffee Rust Resistance
• 4.1 Concepts of Plant Disease Resistance
• 4.2 Mechanisms of Coffee Resistance to H. vastatrix
• 5 Conclusions
• References
• Induced Mutagenesis of in Vitro Tissues and Cells of Coffea arabica L.
• In Vitro Plantlet Establishment of Coffea arabica from Cut Seed Explants
• 1 Introduction
• 2 Materials
• 2.1 Plant Material
• 2.2 Chemicals
• 2.3 Tools and Labware
• 2.4 Lab Equipment
• 3 Methods
• 3.1 Media Preparation
• 3.2 Seed Preparation
• 3.3 Explant Isolation
• 3.4 Surface Sterilization of the Cut Seed
• 3.5 Transfer of Explants and Culture Conditions
• 3.6 Germination and Plantlet Development
• 4 Notes
• References
• Somatic Embryogenesis and Temporary Immersion for Mass Propagation of Chimera-Free Mutant Arabica Coffee Plantlets
• 1 Introduction
• 2 Materials
• 2.1 Plant Material
• 2.2 Supplies, Reagents and Basic Equipment
• 2.3 Equipment
• 2.4 Stock Solutions and Tissue Culture Media
• 3 Methods
• 3.1 Media Preparation
• 3.2 Tissue Collection and Disinfection
• 3.3 In Vitro Leaf Disc Preparation and Primary Callus Induction.
• 3.4 Embryogenic Callus Induction
• 3.5 Cell Multiplication and Establishing the Embryogenic Cell Suspension
• 3.6 Gamma-ray irradiation of the Embryogenic Cell Suspension
• 3.7 Somatic Embryo Differentiation and Germination
• 3.8 Development of Somatic Embryos and Conversion to Plantlets
• 4 Notes
• References
• Protocol on Mutation Induction in Coffee Using In Vitro Tissue Cultures
• 1 Introduction
• 2 Materials
• 2.1 Establishment of a Collection of Donor Material In Vivo
• 2.2 Establishment of In Vitro Shoot Cultures as Donor Material
• 2.3 Establishment of Tissue Culture Material for Mutation Induction
• 2.4 Mutagenesis by Physical Agents
• 3 Methods
• 3.1 Mutagenesis by Physical Agents
• 3.2 Selection and Treatment of Explants
• 3.3 Regeneration of Mutant Plant Lines
• 4 Notes
• References
• Mutation Induction Using Gamma-Ray Irradiation and High Frequency Embryogenic Callus from Coffee (Coffea arabica L.)
• 1 Introduction
• 2 Materials
• 2.1 Plant Material
• 2.2 Explants Collection and Disinfection
• 2.3 Induction of Embryogenic Callus
• 2.4 Embryogenic Callus Multiplication
• 2.5 Embryogenic Callus Irradiation
• 2.6 Regeneration of the Irradiated Embryogenic Callus
• 2.7 Embryo Germination
• 2.8 Development of Somatic Embryos into Plantlets
• 2.9 Media Culture: Preparation
• 3 Methods
• 3.1 Explants Collection and Disinfection
• 3.2 Induction of Embryogenic Callus
• 3.3 Embryogenic Callus Multiplication
• 3.4 Irradiation of the Embryogenic Callus
• 3.5 Regeneration of the Irradiated Embryogenic Callus
• 3.6 Embryo Germination
• 3.7 Regeneration of Plantlets
• 4 Notes
• References
• Chemical Mutagenesis of Embryogenic Cell Suspensions of Coffea arabica L. var. Catuaí Using EMS and NaN3
• 1 Introduction
• 2 Materials
• 2.1 Plant Material
• 2.2 Reagents
• 2.3 Glassware and Minor Equipment.
• 2.4 Equipment
• 2.5 Tissue Culture Media
• 2.6 Software
• 3 Methods
• 3.1 Preparation of Stock Solutions
• 3.2 Preparation of Tissue Culture Media
• 3.3 Germination of Coffee Zygotic Embryos Under In Vitro Conditions
• 3.4 Protocol for Plant Regeneration via Somatic Embryogenesis
• 3.5 Determination of the Viability of the Embryogenic Calli
• 3.6 Sodium Azide Dose Determination
• 3.7 Ethyl Methanesulphonate Dose Determination
• 3.8 Bulk Mutagenesis
• 4 Notes
• References
• Chemical Mutagenesis of Coffea arabica L. var. Venecia Cell Suspensions Using EMS
• 1 Introduction
• 2 Materials
• 2.1 Culture Medium
• 2.2 Chemical Toxicity Test
• 2.3 Calculation of Lethal Dose (LD)
• 3 Methods
• 3.1 Preparation of Liquid Culture Medium
• 3.2 Mutagenesis of Coffee Cell Suspensions: Chemical Toxicity Test
• 3.3 Calculation of Lethal Dose (LD)
• 3.4 Development of Somatic Embryos and Conversion into Plantlets
• 4 Notes
• References
• Chemical Mutagenesis of Zygotic Embryos of Coffea arabica L. var. Catuaí Using EMS and NaN3
• 1 Introduction
• 2 Materials
• 2.1 Plant Material
• 2.2 Reagents
• 2.3 Glassware and Minor Equipment
• 2.4 Equipment
• 2.5 Software
• 2.6 Bulk Mutagenesis of Zygotic Embryos
• 3 Methods
• 3.1 Preparation of Stock Solutions
• 3.2 Preparation of Tissue Culture Medium
• 3.3 Seed Disinfection and Excision of Zygotic Embryos
• 3.4 Determination of the Viability of the Zygotic Embryos
• 3.5 Sodium Azide Dose Determination
• 3.6 Ethyl Methanesulphonate Dose Determination
• 3.7 Bulk Mutagenesis
• 3.8 Acclimatization of M1 Plantlets
• 4 Notes
• References
• Induced Mutagenesis of Seed and Vegetative Propagules of Coffea arabica L.
• Physical Mutagenesis of Arabica Coffee Seeds and Seedlings
• 1 Introduction
• 2 Materials
• 3 Methods
• 3.1 Seed Treatment
• 3.2 Coffee Seedlings Treatment.
• 3.3 Example Radio-Sensitivity Testing of Arabica Coffee Seed Under Laboratory Conditions
• 4 Notes
• References
• Mutation Induction in Coffea arabica L. Using in Vivo Grafting and Cuttings
• 1 Introduction
• 2 Materials
• 2.1 Grafting
• 2.2 Cutting
• 3 Methods
• 3.1 Mutagenesis and Propagation of Coffee Through Bud Grafting
• 3.2 Mutagenesis and Propagation of Coffee Through Cuttings
• 4 Notes
• References
• Chemical Mutagenesis of Mature Seed of Coffea arabica L. var. Venecia Using EMS
• 1 Introduction
• 2 Materials
• 2.1 Chemical Toxicity Test
• 2.2 Calculation of Lethal Dose (LD) and Growth Reduction (GR)
• 2.3 Bulk Mutagenesis
• 2.4 Phenotyping
• 3 Methods
• 3.1 Seed Quality Control and Pre-treatment
• 3.2 Coffee Seed Mutagenesis
• 3.3 Post-treatment Handling of Seeds
• 3.4 Calculation of Lethal Dose (LD): Data Collection and Analyses
• 3.5 Bulk Mutagenesis
• 3.6 Phenotyping
• 4 Notes
• References
• Chemical Mutagenesis of Coffee Seeds (Coffea arabica L. var. Catuaí) Using NaN3
• 1 Introduction
• 2 Materials
• 2.1 Plant Material
• 2.2 Reagents
• 2.3 Glassware and Minor Equipment
• 2.4 Equipment
• 2.5 Software
• 3 Methods
• 3.1 Preparation of Stock Solutions
• 3.2 Seed Germination Test
• 3.3 Sodium Azide Toxicity Test and Dose Optimization
• 3.4 Bulk Mutagenesis
• 3.5 Planting Mutagenized M1 Seedling
• 4 Notes
• References
• Mutant Phenotyping and CLR Resistance Screening Methods
• Use of Open-Source Tools for Imaging and Recording Phenotypic Traits of a Coffee (Coffea arabica L.) Mutant Population
• 1 Introduction
• 2 Materials
• 2.1 Establishment of the C. Arabica Mutant Populations
• 2.2 Phenotyping of the Mutant Populations
• 2.3 Data Analysis Tools
• 3 Methods
• 3.1 Establishing the M1 Mutant Population
• 3.2 Phenotypic Characterisation of the M1 Mutant Population.
• 3.3 Image Analysis to Estimate the Canopy Size
• 3.4 Statistical Analysis
• 3.5 Establishment of M2 Population
• 4 Notes
• References
• Screening for Resistance to Coffee Leaf Rust
• 1 Introduction
• 2 Materials
• 2.1 To Collect and Store Inoculum
• 2.2 Spores Viability
• 2.3 Inoculation
• 2.4 Phenotyping of Coffee-Rust Interactions
• 3 Methods
• 3.1 Procedures to Collect and Store Inoculum
• 3.2 Spores Germination Tests
• 3.3 Inoculation Techniques
• 3.4 Phenotypic Scoring Method for Disease Resistance
• 4 Notes
• References
• Inoculation and Evaluation of Hemileia vastatrix Under Laboratory Conditions
• 1 Introduction
• 2 Materials
• 2.1 Preparation of Rust Inoculum
• 2.2 Rust Inoculation
• 2.3 Rust Evaluation
• 3 Methods
• 3.1 Preparation of Rust Inocula
• 3.2 Rust Inoculation
• 3.3 Rust Evaluation
• 4 Notes
• References
• Evaluation of Coffee (Coffea arabica L. var. Catuaí) Tolerance to Leaf Rust (Hemileia vastatrix) Using Inoculation of Leaf Discs Under Controlled Conditions
• 1 Introduction
• 2 Materials
• 2.1 Plant Material
• 2.2 Other Biological Materials
• 2.3 Consumables and Minor Equipment
• 2.4 Reagents and Agrochemicals
• 2.5 Equipment
• 3 Methods
• 3.1 Germination of M2 Seeds
• 3.2 Planting Seedlings M2
• 3.3 Preparation of Coffee Leaf Rust Inoculum
• 3.4 Inoculation of the Coffee Leaf Discs with CLR
• 3.5 Evaluation of Plant Resistance Against CLR
• 4 Notes
• References
• A PCR-Based Assay for Early Diagnosis of the Coffee Leaf Rust Pathogen Hemileia vastatrix
• 1 Introduction
• 2 Materials
• 3 Methods
• 3.1 Designing the Specific Primers for Hemileia vastatrix
• 3.2 Total DNA Extraction from Suspected Diseased Leaves or Typical Diseased Samples
• 3.3 Preparation of the PCR Reaction Mixture and PCR Amplification
• 3.4 Gel Electrophoresis
• 4 Notes
• References.
• Molecular Characterisation of Induced Mutations in Coffee.