Mutation Breeding in Coffee with Special Reference to Leaf Rust : : Protocols.

• Author: Ingelbrecht, Ivan L. W.
• TeilnehmendeR: Silva, Maria do Céu Lavado da.; Jankowicz-Cieslak, Joanna.
• Place / Publishing House: Berlin, Heidelberg : : Springer Berlin / Heidelberg,, 2023.; ©2023.
• Year of Publication: 2023
• Edition: 1st ed.
• Language: English
• Physical Description: 1 online resource (316 pages)

Table of Contents

Intro -- Preface -- Acknowledgements -- Contents -- Contributors -- Introduction -- Mutation Breeding in Arabica Coffee -- 1 General Principles of Plant Mutation Breeding -- 2 Breeding Limitations in Arabica Coffee -- 3 Mutation Breeding in Arabica Coffee -- 3.1 Background -- 3.2 The Need for Radiosensitivity Testing -- 3.3 Choice of Material for Mutation Induction -- 3.4 Enabling Biotechnology and Genomics Tools -- References -- Coffee Leaf Rust Resistance: An Overview -- 1 Introduction -- 2 Coffee's Main Diseases -- 3 CLR Causal Agent: Hemileia vastatrix -- 3.1 Life Cycle and Infection Process -- 3.2 Genetic and Physiological Diversity -- 3.3 Implications of Rust Molecular Diversity and Population Dynamics in Coffee Breeding -- 4 Coffee Rust Resistance -- 4.1 Concepts of Plant Disease Resistance -- 4.2 Mechanisms of Coffee Resistance to H. vastatrix -- 5 Conclusions -- References -- Induced Mutagenesis of in Vitro Tissues and Cells of Coffea arabica L. -- In Vitro Plantlet Establishment of Coffea arabica from Cut Seed Explants -- 1 Introduction -- 2 Materials -- 2.1 Plant Material -- 2.2 Chemicals -- 2.3 Tools and Labware -- 2.4 Lab Equipment -- 3 Methods -- 3.1 Media Preparation -- 3.2 Seed Preparation -- 3.3 Explant Isolation -- 3.4 Surface Sterilization of the Cut Seed -- 3.5 Transfer of Explants and Culture Conditions -- 3.6 Germination and Plantlet Development -- 4 Notes -- References -- Somatic Embryogenesis and Temporary Immersion for Mass Propagation of Chimera-Free Mutant Arabica Coffee Plantlets -- 1 Introduction -- 2 Materials -- 2.1 Plant Material -- 2.2 Supplies, Reagents and Basic Equipment -- 2.3 Equipment -- 2.4 Stock Solutions and Tissue Culture Media -- 3 Methods -- 3.1 Media Preparation -- 3.2 Tissue Collection and Disinfection -- 3.3 In Vitro Leaf Disc Preparation and Primary Callus Induction.

3.4 Embryogenic Callus Induction -- 3.5 Cell Multiplication and Establishing the Embryogenic Cell Suspension -- 3.6 Gamma-ray irradiation of the Embryogenic Cell Suspension -- 3.7 Somatic Embryo Differentiation and Germination -- 3.8 Development of Somatic Embryos and Conversion to Plantlets -- 4 Notes -- References -- Protocol on Mutation Induction in Coffee Using In Vitro Tissue Cultures -- 1 Introduction -- 2 Materials -- 2.1 Establishment of a Collection of Donor Material In Vivo -- 2.2 Establishment of In Vitro Shoot Cultures as Donor Material -- 2.3 Establishment of Tissue Culture Material for Mutation Induction -- 2.4 Mutagenesis by Physical Agents -- 3 Methods -- 3.1 Mutagenesis by Physical Agents -- 3.2 Selection and Treatment of Explants -- 3.3 Regeneration of Mutant Plant Lines -- 4 Notes -- References -- Mutation Induction Using Gamma-Ray Irradiation and High Frequency Embryogenic Callus from Coffee (Coffea arabica L.) -- 1 Introduction -- 2 Materials -- 2.1 Plant Material -- 2.2 Explants Collection and Disinfection -- 2.3 Induction of Embryogenic Callus -- 2.4 Embryogenic Callus Multiplication -- 2.5 Embryogenic Callus Irradiation -- 2.6 Regeneration of the Irradiated Embryogenic Callus -- 2.7 Embryo Germination -- 2.8 Development of Somatic Embryos into Plantlets -- 2.9 Media Culture: Preparation -- 3 Methods -- 3.1 Explants Collection and Disinfection -- 3.2 Induction of Embryogenic Callus -- 3.3 Embryogenic Callus Multiplication -- 3.4 Irradiation of the Embryogenic Callus -- 3.5 Regeneration of the Irradiated Embryogenic Callus -- 3.6 Embryo Germination -- 3.7 Regeneration of Plantlets -- 4 Notes -- References -- Chemical Mutagenesis of Embryogenic Cell Suspensions of Coffea arabica L. var. Catuaí Using EMS and NaN3 -- 1 Introduction -- 2 Materials -- 2.1 Plant Material -- 2.2 Reagents -- 2.3 Glassware and Minor Equipment.

2.4 Equipment -- 2.5 Tissue Culture Media -- 2.6 Software -- 3 Methods -- 3.1 Preparation of Stock Solutions -- 3.2 Preparation of Tissue Culture Media -- 3.3 Germination of Coffee Zygotic Embryos Under In Vitro Conditions -- 3.4 Protocol for Plant Regeneration via Somatic Embryogenesis -- 3.5 Determination of the Viability of the Embryogenic Calli -- 3.6 Sodium Azide Dose Determination -- 3.7 Ethyl Methanesulphonate Dose Determination -- 3.8 Bulk Mutagenesis -- 4 Notes -- References -- Chemical Mutagenesis of Coffea arabica L. var. Venecia Cell Suspensions Using EMS -- 1 Introduction -- 2 Materials -- 2.1 Culture Medium -- 2.2 Chemical Toxicity Test -- 2.3 Calculation of Lethal Dose (LD) -- 3 Methods -- 3.1 Preparation of Liquid Culture Medium -- 3.2 Mutagenesis of Coffee Cell Suspensions: Chemical Toxicity Test -- 3.3 Calculation of Lethal Dose (LD) -- 3.4 Development of Somatic Embryos and Conversion into Plantlets -- 4 Notes -- References -- Chemical Mutagenesis of Zygotic Embryos of Coffea arabica L. var. Catuaí Using EMS and NaN3 -- 1 Introduction -- 2 Materials -- 2.1 Plant Material -- 2.2 Reagents -- 2.3 Glassware and Minor Equipment -- 2.4 Equipment -- 2.5 Software -- 2.6 Bulk Mutagenesis of Zygotic Embryos -- 3 Methods -- 3.1 Preparation of Stock Solutions -- 3.2 Preparation of Tissue Culture Medium -- 3.3 Seed Disinfection and Excision of Zygotic Embryos -- 3.4 Determination of the Viability of the Zygotic Embryos -- 3.5 Sodium Azide Dose Determination -- 3.6 Ethyl Methanesulphonate Dose Determination -- 3.7 Bulk Mutagenesis -- 3.8 Acclimatization of M1 Plantlets -- 4 Notes -- References -- Induced Mutagenesis of Seed and Vegetative Propagules of Coffea arabica L. -- Physical Mutagenesis of Arabica Coffee Seeds and Seedlings -- 1 Introduction -- 2 Materials -- 3 Methods -- 3.1 Seed Treatment -- 3.2 Coffee Seedlings Treatment.

3.3 Example Radio-Sensitivity Testing of Arabica Coffee Seed Under Laboratory Conditions -- 4 Notes -- References -- Mutation Induction in Coffea arabica L. Using in Vivo Grafting and Cuttings -- 1 Introduction -- 2 Materials -- 2.1 Grafting -- 2.2 Cutting -- 3 Methods -- 3.1 Mutagenesis and Propagation of Coffee Through Bud Grafting -- 3.2 Mutagenesis and Propagation of Coffee Through Cuttings -- 4 Notes -- References -- Chemical Mutagenesis of Mature Seed of Coffea arabica L. var. Venecia Using EMS -- 1 Introduction -- 2 Materials -- 2.1 Chemical Toxicity Test -- 2.2 Calculation of Lethal Dose (LD) and Growth Reduction (GR) -- 2.3 Bulk Mutagenesis -- 2.4 Phenotyping -- 3 Methods -- 3.1 Seed Quality Control and Pre-treatment -- 3.2 Coffee Seed Mutagenesis -- 3.3 Post-treatment Handling of Seeds -- 3.4 Calculation of Lethal Dose (LD): Data Collection and Analyses -- 3.5 Bulk Mutagenesis -- 3.6 Phenotyping -- 4 Notes -- References -- Chemical Mutagenesis of Coffee Seeds (Coffea arabica L. var. Catuaí) Using NaN3 -- 1 Introduction -- 2 Materials -- 2.1 Plant Material -- 2.2 Reagents -- 2.3 Glassware and Minor Equipment -- 2.4 Equipment -- 2.5 Software -- 3 Methods -- 3.1 Preparation of Stock Solutions -- 3.2 Seed Germination Test -- 3.3 Sodium Azide Toxicity Test and Dose Optimization -- 3.4 Bulk Mutagenesis -- 3.5 Planting Mutagenized M1 Seedling -- 4 Notes -- References -- Mutant Phenotyping and CLR Resistance Screening Methods -- Use of Open-Source Tools for Imaging and Recording Phenotypic Traits of a Coffee (Coffea arabica L.) Mutant Population -- 1 Introduction -- 2 Materials -- 2.1 Establishment of the C. Arabica Mutant Populations -- 2.2 Phenotyping of the Mutant Populations -- 2.3 Data Analysis Tools -- 3 Methods -- 3.1 Establishing the M1 Mutant Population -- 3.2 Phenotypic Characterisation of the M1 Mutant Population.

3.3 Image Analysis to Estimate the Canopy Size -- 3.4 Statistical Analysis -- 3.5 Establishment of M2 Population -- 4 Notes -- References -- Screening for Resistance to Coffee Leaf Rust -- 1 Introduction -- 2 Materials -- 2.1 To Collect and Store Inoculum -- 2.2 Spores Viability -- 2.3 Inoculation -- 2.4 Phenotyping of Coffee-Rust Interactions -- 3 Methods -- 3.1 Procedures to Collect and Store Inoculum -- 3.2 Spores Germination Tests -- 3.3 Inoculation Techniques -- 3.4 Phenotypic Scoring Method for Disease Resistance -- 4 Notes -- References -- Inoculation and Evaluation of Hemileia vastatrix Under Laboratory Conditions -- 1 Introduction -- 2 Materials -- 2.1 Preparation of Rust Inoculum -- 2.2 Rust Inoculation -- 2.3 Rust Evaluation -- 3 Methods -- 3.1 Preparation of Rust Inocula -- 3.2 Rust Inoculation -- 3.3 Rust Evaluation -- 4 Notes -- References -- Evaluation of Coffee (Coffea arabica L. var. Catuaí) Tolerance to Leaf Rust (Hemileia vastatrix) Using Inoculation of Leaf Discs Under Controlled Conditions -- 1 Introduction -- 2 Materials -- 2.1 Plant Material -- 2.2 Other Biological Materials -- 2.3 Consumables and Minor Equipment -- 2.4 Reagents and Agrochemicals -- 2.5 Equipment -- 3 Methods -- 3.1 Germination of M2 Seeds -- 3.2 Planting Seedlings M2 -- 3.3 Preparation of Coffee Leaf Rust Inoculum -- 3.4 Inoculation of the Coffee Leaf Discs with CLR -- 3.5 Evaluation of Plant Resistance Against CLR -- 4 Notes -- References -- A PCR-Based Assay for Early Diagnosis of the Coffee Leaf Rust Pathogen Hemileia vastatrix -- 1 Introduction -- 2 Materials -- 3 Methods -- 3.1 Designing the Specific Primers for Hemileia vastatrix -- 3.2 Total DNA Extraction from Suspected Diseased Leaves or Typical Diseased Samples -- 3.3 Preparation of the PCR Reaction Mixture and PCR Amplification -- 3.4 Gel Electrophoresis -- 4 Notes -- References.

Molecular Characterisation of Induced Mutations in Coffee.