CRISPR Technology : : recent advances / / edited by Yuan-Chuan Chen.
CRISPR technology has been extensively used in vitro and in vivo as a tool in basic research for genetic editing (e.g., genome encoding, silencing, enhancing, and modification). Although there are many technical and ethical challenges to overcome, such as off-target effects, delivery tool selection,...
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Place / Publishing House: | London : : IntechOpen,, 2023. ©2023 |
Year of Publication: | 2023 |
Language: | English |
Physical Description: | 1 online resource (158 pages) |
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Table of Contents:
- Preface
- Section 1 Introduction
- Chapter 1 Introductory Chapter: CRISPR Technology by Yuan-Chuan Chen
- Chapter 2 Emerging CRISPR Technologies by Annelise Cassidy and Stephane Pelletier
- Section 2 Technology
- Chapter 3 Maximizing the Efficacy of CRISPR/Cas Homology-Directed Repair Gene Targeting by Terry S. Elton, Md. Ismail Hossain, Jessika Carvajal-Moreno, Xinyi Wang, Dalton J. Skaggs and Jack C. Yalowich
- Chapter 4 The Prominent Characteristics of the Effective sgRNA for a Precise CRISPR Genome Editing by Reza Mohammadhassan, Sara Tutunchi, Negar Nasehi, Fatemeh Goudarziasl and Lena Mahya
- Chapter 5 Recent Advances in In Vivo Genome Editing Targeting Mammalian Preimplantation Embryos by Masahiro Sato, Masato Ohtsuka, Emi Inada, Shingo Nakamura, Issei Saitoh and Shuji Takabayashi
- Section 3 Application
- Chapter 6 Applications of CRISPR/Cas9 for Selective Sequencing and Clinical Diagnostics by Maximilian Evers, Björn Brändl, Franz-Josef Müller, Sönke Friedrichsen and Stephan Kolkenbrock.