Low-Cost Methods for Molecular Characterization of Mutant Plants : : Tissue Desiccation, DNA Extraction and Mutation Discovery: Protocols / / by Bradley J. Till, Joanna Jankowicz-Cieslak, Owen A. Huynh, Mayada M. Beshir, Robert G. Laport, Bernhard J. Hofinger.
This book offers low-cost and rapid molecular assays for the characterization of mutant plant germplasm. Detailed protocols are provided for the desiccation of plant tissues; the extraction of high-quality DNA for downstream applications; the extraction of single-strand-specific nucleases for single...
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| Place / Publishing House: | Cham : : Springer International Publishing :, Imprint: Springer,, 2015. |
| Year of Publication: | 2015 |
| Edition: | 1st ed. 2015. |
| Language: | English |
| Physical Description: | 1 online resource (X, 35 p. 9 illus., 3 illus. in color.) |
| Notes: | Bibliographic Level Mode of Issuance: Monograph |
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Table of Contents:
- Introduction
- Health and Safety Considerations
- Sample Collection and Storage
- Low-Cost DNA Extraction
- PCR Amplification for Low-Cost Mutation Discovery
- Enzymatic Mismatch Cleavage and Agarose Gel Evaluation of Samples
- Alternative Enzymology for Mismatch Cleavage for TILLING and Ecotilling: Extraction of Enzymes form Common Weedy Plants
- Example Data
- Conclusions. .