Innovative strategies in tissue engineering / / editors, Mayuri Prasad, Paolo di Nardo.

• Superior document: River Publishers Series in Research and Business Chronicles: Biotechnology and Medicine ; Volume 2
• TeilnehmendeR: Prasad, Mayuri,; Di Nardo, Paolo,
• Place / Publishing House: Aalborg, Denmark : : River Publishers,, 2015.; ©2015
• Year of Publication: 2015
• Edition: 1st ed.
• Language: English
• Series: River publishers series in research and business chronicles: biotechnology and medicine ; Volume 2.
• Physical Description: 1 online resource (211 pages) :; illustrations (some color), charts, photographs, graphs.

In spite of intensive investments and investigations carried out in the last decade, many aspects of the stem cell physiology, technology and regulation remain to be fully defined. After the enthusiasm that characterized the first decade of the discovery that when given the right cue, stem cells could repair all the different tissues in the body; it is now time to start a serious and coordinated action to define how to govern the stem cell potential and to exploit it for clinical applications. This can be achieved only with shared research programs involving investigators from all over the world and making the results available to all.The Disputationes Workshop series (http://disputationes.info) is an international initiative aimed at disseminating stem cell related cutting edge knowledge among scientists, healthcare workers, students and policy makers. The present book gathers together some of the ideas discussed during the third and fourth Disputationes Workshops held in Florence (Italy) and Aalborg (Denmark), respectively. The aim of this book is to preserve those ideas in order to contribute to the general discussion on organ repair and to bolster a fundamental scientific and technological leap forwards the treatment of otherwise incurable diseases.

Table of Contents

Cover -- Half Title -- Title -- Copyright -- Contents -- Preface -- List of Figures -- List of Abbreviations -- 1. Bioactive Nanocomposites withApplications in Biomedicine -- Abstract -- 1.1 Introduction -- 1.2 Factors that Influence the Quality of a Biomaterial -- 1.3 Layered Silicate Nanocomposites for Biomaterials -- 1.3.1 Layered Silicate Properties -- 1.3.2 Layered Silicate Purification -- 1.3.3 Layered Silicate in Drug Release Systems -- 1.3.4 Biopolymers Properties -- 1.4 Routes for Obtaining Bio-Nanocomposites -- 1.5 Biomaterials Development -- 1.6 Conclusions -- References -- 2. Cerium Dioxide Nanoparticles ProtectCardiac Progenitor Cells against theOxidative Stress -- Abstract -- 2.1 Interaction of Cerium Oxide Nanoparticleswith Biological Systems -- 2.2 Cerium Oxide Nanoparticles Shield Cardiac PrecursorCells against the Oxidative Stress -- References -- 3. Animals Models and In Vitro Alternativesin Regenerative Medicine: Focus onBiomaterials Development -- 3.1 Introduction -- 3.1.1 Animals in Medical Research -- 3.1.2 Ethical Considerations of Animal Use -- 3.2 Designing Animal Experiments -- 3.2.1 Randomization and Blinding -- 3.2.2 Control Groups -- 3.2.3 Statistical Analysis -- 3.2.4 Design Stages -- 3.3 Limitations of Animal Models -- 3.3.1 Animal Species -- 3.3.2 Health and Age Status -- 3.3.3 Reproducibility -- 3.4 Examples of Animal Models for Cardiac and CornealRegenerative Medicine Testing -- 3.4.1 Myocardial Infarct and Other Ischemic Models -- 3.4.1.1 Myocardial coronary artery ligation -- 3.4.1.3 Hind-limb ischemia -- 3.4.1.2 Cryoinjury -- 3.4.2 Corneal Transplantation Models -- 3.4.2.1 Animal species -- 3.4.2.2 Lamellar and penetrating keratoplasty -- 3.4.2.3 Infectious models -- 3.5 In Vitro Systems as Alternatives to Animal Testing -- 3.5.1 In Vitro Corneal Equivalents for Screening Biomaterialsas Potential Implants.

3.5.2 In Vitro Angiogenesis Models -- 3.6 Conclusion -- References -- 4. Differentiation Plasticity of GermlineCell-Derived Pluripotent Stem Cells andTheir Potential Application in RegenerativeMedic -- Abstract -- 4.1 Introduction -- 4.2 Hepatocytes Derived from GPSCs -- 4.3 Cardiac Cells Derived from GPSCs -- 4.4 Neuronal Cells Derived from GPSCs -- 4.5 Hematopoietic Cells from GPSCs -- 4.6 Vascular Cells Derived from GPSCs -- References -- 5. Mechanical Stimulation in TissueEngineering -- 5.1 Background and Introduction -- 5.1.1 Mechanical Theories of Material Damage -- 5.1.2 Damage of Living Tissue -- 5.2 Mechanical Loading in Two Dimensions -- 5.2.1 Hertz-inspired Tissue Deformation -- 5.2.2 Preliminary Results of Cell Straining -- 5.3 Conclusions and Outlook -- References -- 6. Immune Properties of Mesenchymal StemCells in the Translation of Neural Disorders -- Abstract -- 6.1 Introduction -- 6.2 MSC Immunology -- 6.3 MSCs and Cancer -- 6.3.1 Role in Tumor Growth -- 6.3.2 MSCs in Tumor Suppression -- 6.3.3 MSC and Brain Cancer -- 6.4 Regenarative Potential -- 6.5 Safety -- 6.6 Conclusion -- Refrences -- 7. Novel Design of Manufacturing Bioreactorand Facility of Cell-Based Health CareProducts for Regenerative Medicine -- Abstract -- 7.1 Introduction -- 7.2 Bioreactor Design for Cell Processing -- 7.3 Facility Design for Cell Processing -- 7.4 Flexible Modular Platform Technology -- 7.5 Acknowledgments -- References -- 8. Insight into Melanoma Stem Cells:The Roleof the Hedgehog Signaling in RegulatingSelf-Renewal and Tumorigenicity -- 8.1 Introduction -- 8.2 Evidence for the Existance of Melanoma Stem Cellswith Self-Renewing and Tumorigenic Properties -- 8.3 The Hedgehog Signaling Pathway -- 8.4 Role of the Hedgehog Signaling in RegulatingSelf-Renewal and Tumorigenicity of MelanomaStem Cells -- 8.5 Conclusions -- 8.6 Acknowledgement.

References -- 9. A Quest for Refocussing Stem CellInduction Strategies: How to Deal withEthical Objections and Patenting Problems -- Abstract -- 9.1 Introduction -- 9.2 Potential for Autonomous Pattern Formation:Embryoid Bodies -- 9.3 Potential for Assisted Development:TetraploidComplementation -- 9.4 Pluripotency, an Obstacle for Patenting -- 9.5 Alternative Approaches -- 9.6 Conclusions -- 9.7 Acknowledgments -- References -- 10. Constitutive Equations in FiniteViscoplasticity of Nanocomposite Hydrogels -- 10.1 Introduction -- 10.2 Constitutive Model -- 10.2.1 Kinematic Relations -- 10.2.2 Free Energy Density of a Hydrogel -- 10.2.3 Derivation of Constitutive Equations -- 10.3 Simplification of the Constitutive Equations -- 10.4 Fitting of Observations -- 10.4.1 Nanocomposite Hydrogels Subjected to Dryingand Swelling -- 10.4.2 As-Prepared Poly(Dimethylacrylamide)-Silica Hydrogels -- 10.4.3 As-Prepared Polyacrylamide-Clay Hydrogels -- 10.4.4 Discussion -- 10.5 Concluding Remarks -- 10.6 Acknowledgement -- References -- 11. Regulatory Issues in Developing AdvancedTherapy Medicinal Products withStem Cells in Europe -- 11.1 Introduction -- 11.2 European Regulatory Frame for ATMP -- 11.3 Stem Cell-Based ATMP -- 11.4 Quality Issues for Stem Cell-Based ProductDevelopment -- 11.5 Non Clinical Issues for Stem Cell-Based ProductDevelopment -- 11.6 Clinical Issues for Stem Cell-Based ProductDevelopment -- 11.7 Conclusion -- References -- Index -- Editor's Biographies.