The stem cell microenvironment and its role in regenerative medicine and cancer pathogenisis / / editors, Cristian Pablo Pennisi, Mayuri Sinha Prasad, Pranela Rameshwar.

• Superior document: River Publishers series in research and business chronicles: biotechnology and medicine ; Volume 7
• Author: Disputationes Workshop
• TeilnehmendeR: Pennisi, Cristian Pablo,; Prasad, Mayuri,; Rameshwar, Pranela,; Disputationes Workshop (5th : 2014 : Ålborg, Denmark)
• Place / Publishing House: Gistrup, Denmark ;, Delft, The Netherlands : : River Publishers,, 2017.; ©2017
• Year of Publication: 2017
• Edition: First edition.
• Language: English
• Series: River publishers series in research and business chronicles: biotechnology and medicine ; Volume 7.
• Physical Description: 1 online resource (144 pages) :; illustrations (some color), tables.
• Notes: "This book emerges as a result of the scientific contributions presented during the fifth Disputationes Workshop held in Aalborg (Denmark) in April 2014 and discusses some of the recent advances in stem cell research that may help understanding the properties of the niche that govern stem cell fate"--Page ix.

How stem cells behave is very much a factor of their local microenvironment, also known as the stem cell niche. Physical, chemical, or electrical signals from the neighboring cells or biochemical signals from distant cells are crucial in the cell fate decision process. A major challenge of tissue engineering is to mimic the natural cell environment by designing very sophisticated scaffolds able not only to mechanically support cells, but also to release signals biologically relevant for governing stem cell fate. In addition, increasing evidence suggests that abnormal interaction of stem cells with their niche is responsible for altered cell function leading to malignant transformation. This book discusses some of the recent advances in stem cell research that may help understanding the properties of the niche that govern stem cell fate. Technical topics discussed include:Stem cell biologyCancer stem cellsStem cell interactions with biomaterialsEngineering the stem cell microenvironmentStem cells in tissue regeneration and repairThe Disputationes Workshop series is an international initiative aimed at disseminating stem cell related cutting edge knowledge among scientists, healthcare workers, students and policy makers. This book emerges as a result of the scientific contributions presented and discussed during the fifth Disputationes Workshop held in Aalborg (Denmark) in April 2014. The stem cell microenvironment and its role in regenerative medicine and cancer pathogenesis is ideal for academic staff and master/research students in biomedical and health sciences

Table of Contents

Front Cover -- Half Title Page -- RIVER PUBLISHERS SERIES IN RESEARCH AND BUSINESS CHRONICLES: BIOTECHNOLOGY AND MEDICINE -- Full Title - The Stem Cell Microenvironment and Its Role in Regenerative Medicine and Cancer Pathogenesis -- Copyright Page -- Contents -- Preface -- List of Contributors -- List of Figures -- List of Tables -- List of Abbreviations -- Chapter 1 - Selective Expansion of Limbal Epithelial Stem Cells in Culture Using Hypoxia -- 1.1 Introduction -- 1.2 Clinical Application of Bioengineered Corneal Epithelial Stem Cell Grafts -- 1.3 Culture Techniques for Bioengineered Stem Cell Sheets -- 1.4 Selective Expansion of LESCs Using Hypoxia -- 1.5 Future Perspectives -- References -- Chapter 2 - Mesenchymal Stem Cells and Pathotropism: Regenerative Potential and Safety Concerns -- 2.1 Introduction -- 2.2 Mesenchymal Stem Cells (MSC) -- 2.2.1 MSC Immunology -- 2.3 MSC in Tumor Support -- 2.3.1 MSC in Drug Delivery to Tumors -- 2.4 Conclusion -- References -- Chapter 3 - Prospective Technologies for Cardiac Repair -- 3.1 Medicine Changing Needs -- 3.2 Additive Technologies in Tissue Engineering -- References -- Chapter 4 - Does Inter-Individual Heterogeneity in the Normal Breast Corrupt Cancer Stem Cell and/or Cancer-Specific Signaling Characterization? -- 4.1 Introduction -- 4.2 Defining Normal Breast Hierarchy -- 4.3 Need for In Vitro Assays to Document Inter-Individual Heterogeneity in the Normal Breast -- 4.4 Future Directions -- Acknowledgements -- References -- Chapter 5 - The Role of Physical Microenvironmental Cues on Myogenesis: Implications for Tissue Engineering of Skeletal Muscle -- 5.1 Introduction -- 5.2 In Vitro Models of Adult Myogenesis -- 5.3 Effect of Soluble and Bound Biochemical Cues -- 5.4 Regulation of Cell Fate by Passive Physical Cues -- 5.4.1 Substrate Topography -- 5.4.2 Substrate Stiffness.

5.5 Active Stimulation -- 5.5.1 Electrical Stimulation -- 5.5.2 Mechanical Loading -- 5.6 Summary and Perspectives -- References -- Chapter 6 - Effect of Bioactive Growth Surfaces on Human Mesenchymal Stem Cells: A Pilot Biomarker Study to Assess Growth and Differentiation -- 6.1 Introduction -- 6.2 Materials and Methods -- 6.2.1 Materials -- 6.2.2 Cell Culture Reagents -- 6.2.3 Culture of Human MSCs -- 6.2.4 qPCR for Stem Cell Markers -- 6.3 Results -- 6.4 Discussion -- 6.5 Conclusions -- Acknowledgements -- References -- Chapter 7 - Embryonic Stem Cell Markers in Cancer: Cripto-1 Expression in Glioblastoma -- 7.1 Introduction -- 7.2 Glioma Stem Cells (GSC) -- 7.3 A New Cancer Stem Cell Marker in the Tumor Scaffold -- 7.4 Cr-1 Expression in GBMTissue and the Angiogenic Phenotype -- 7.5 CR-1 Expression Linked to Poorer Prognosis and Shorter Survival -- 7.6 Conclusion -- References -- Chapter 8 Artificial Corneas, and Reinforced Composite Implants for High Risk Donor Cornea Transplantation -- 8.1 Introduction -- 8.1.1 Cornea Transplantation -- 8.2 Artificial Corneas as Prostheses and Regeneration Templates -- 8.2.1 Artificial Corneas as Prostheses -- 8.2.2 Artificial Corneas as Regeneration Templates -- 8.3 Reinforced Collagen Corneal Implants -- 8.3.1 Interpenetrating Networks of Collagen-Phosphorylcholine as Implants -- 8.3.2 RHCIII-MPC Implants in Herpes Simplex Keratitis -- 8.4 Composite Corneal Implants with Peptide and Gene Therapy Capacity -- 8.4.1 LL-37 -- 8.4.2 Implants LL-37 Peptide Release -- 8.4.3 Composite Collagen-Cell-Based Implants -- 8.5 Conclusion -- Ackowledgements -- References -- Chapter 9 - Molecular Mechanisms of Smooth Muscle Cell Differentiation from Adipose-Derived Stem Cell -- 9.1 Introduction -- 9.2 SMC and ASC Characterization -- 9.3 Differentiation of SMC from ASC and Possible Molecular Mechanism.

9.4 Conclusion and Perspectives -- 9.5 Conflict of Interest -- References -- Index -- About the Editors -- Back Cover.