Enzymes and Biocatalysis / / Chia-Hung Kuo [and three others].

• VerfasserIn: Kuo, Chia-Hung,
• Place / Publishing House: Basel : : MDPI - Multidisciplinary Digital Publishing Institute,, 2022.
• Year of Publication: 2022
• Language: English
• Physical Description: 1 online resource (244 pages)

Enzymes, also known as biocatalysts, are proteins produced by living cells and found in a wide range of species, including animals, plants, and microorganisms. Due to their specificity, enzymes are often widely used to catalyze various chemical reactions as proficient biocatalysts in many applications. Since enzymes are proteins, they are easily denatured and lose their activity under unfavorable conditions of the surrounding environment. Therefore, in order to prolong the activity of and increase the stability of enzymes, immobilization technology is commonly used for improving the overall efficiency of enzyme catalysis. Recent developments in the discovery of stable enzymes have expanded their uses to encompass organic synthesis and the production of specialized chemicals, pharmaceutical intermediates, and agrochemicals. In order to improve enzyme performance, microbial screening or gene cloning is generally used. It is also common for enzyme studies to use a particular medium for microbiological fermentation in order to produce enzymes. This Special Issue shows that enzymes and biocatalysis have found numerous applications in various fields. These applications include the production of APIs, wastewater treatment, transformation of health ingredients, laundry detergent, fortification of feed, and biocementation. Moreover, there are also studies focusing on the immobilization and production procedures of enzymes. Finally, an extensive review of metagenomics studies related to water and soil has also been included.

Table of Contents

About the Editors -- Enzymes and Biocatalysis -- Two Novel, Flavin-Dependent Halogenases from the Bacterial Consortia of Botryococcus braunii Catalyze Mono- and Dibromination -- Exogenous Enzymes as Zootechnical Additives in Animal Feed: A Review -- Use of a Sequential Fermentation Method for the Production of Aspergillus tamarii URM4634 Protease and a Kinetic/ Thermodynamic Study of the Enzyme -- Utilization of Clay Materials as Support for Aspergillus japonicus Lipase: An Eco-Friendly Approach -- Phenylalanine, Tyrosine, and DOPA Are bona fide Substrates for Bambusa oldhamii BoPAL4 -- Gordonia hydrophobica Nitrile Hydratase for Amide Preparation from Nitriles -- Effects of Lower Temperature on Expression and Biochemical Characteristics of HCV NS3 Antigen Recombinant Protein -- Application Potential of Cyanide Hydratase from Exidia glandulosa: Free Cyanide Removal from Simulated Industrial Effluents -- An Alkalothermophilic Amylopullulanase from the Yeast Clavispora lusitaniae ABS7: Purification, Characterization and Potential Application in Laundry Detergent -- Effects of Soil Surface Chemistry on Adsorption and Activity of Urease from a Crude Protein Extract: Implications for Biocementation Applications -- Metagenomic Approaches as a Tool to Unravel Promising Biocatalysts from Natural Resources: Soil and Water -- Continuous Production of DHA and EPA Ethyl Esters via Lipase-Catalyzed Transesterification in an Ultrasonic Packed-Bed Bioreactor -- Rational Design of a Calcium-Independent Trypsin Variant.