Ruolo di TAF12B e UVR3 nel ciclo circadiano dei vegetali / / Susanna Pollastri.

The book goes over the studies carried out on the circadian oscillators in plants, performed using the model plant <i>Arabidopsis thaliana</i>. The plant's oscillators seem to include similar components to those described for other model organisms. A bioinformatic analysis on the Ar...

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Bibliographic Details
Superior document:Premio Tesi di Dottorato ; 28
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Place / Publishing House:Firenze : : Firenze University Press,, 2012
Year of Publication:2012
Language:English
Series:Premio Tesi di Dottorato ; 28.
Physical Description:1 online resource (78 pages) :; illustrations; digital, PDF file(s).
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520 |a The book goes over the studies carried out on the circadian oscillators in plants, performed using the model plant <i>Arabidopsis thaliana</i>. The plant's oscillators seem to include similar components to those described for other model organisms. A bioinformatic analysis on the Arabidopsis genome found the genes TAF12b, At1g17440 and UVR3, At3g15620. Both the genes were cloned with the GFP green fluorescent protein in order to locate this protein at cell level. Furthermore, Arabidopsis SALK line plants were grown and selected, mutating the genes in question, and in parallel gene expression tests were performed on the main circadian rhythm genes in order to monitor their expression both in conditions of alternating light and dark, and in constant conditions. From the data that has emerged to date it seems that both genes are involved in the Arabidopsis's circadian rhythms. 
530 |a Also available in print form. 
505 0 |a Lista delle abbreviazioni -- Capitolo 1 -- Introduzione -- 1. I ritmi circadiani 1 -- 2. Allineamento dei ritmi interni ed esterni nello sviluppo di Arabidopsis 2 -- 3. Le proprietà dell'orologio circadiano 3 -- 4. Il motivo della presenza dell'orologio 5 -- 5. L'architettura dell'orologio 5 6. Il nucleo oscillatore 6 -- 7. Basi molecolari della regolazione dei geni orologio 8 -- 8. Componenti aggiuntivi del sistema circadiano 8 -- 9. Studio dei ritmi circadiani in Arabidopsis 10 -- 10. Obiettivi della ricerca 11 -- Capitolo 2 -- Materiali e metodi -- 1. Materiali 13 -- 2. Metodi 14 -- Capitolo 3 -- Risultati -- 1. Identificazione omologo di CLOCK, Homo sapiens, in Arabidopsis thaliana 21 -- 2. Identificazione omologo di CRYPTOCROMO 1, Homo sapiens, in Arabidopsis thaliana 27 -- 3. Analisi dell'espressione genica 36Il ruolo di TAF12B e UVR3 nel ciclo circadiano dei vegetali -- Capitolo 4 -- Discussione -- 1. TAF12B 43 -- 2. UVR3 44 -- 3. Conclusioni 46 -- Appendice 47 -- Bibliografia 59 -- Ringraziamenti 66. 
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830 0 |a Premio Tesi di Dottorato ;  |v 28. 
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