Single Cell Analysis in Biotechnology and Systems Biology / / MDPI - Multidisciplinary Digital Publishing Institute.

• VerfasserIn: MDPI - Multidisciplinary Digital Publishing Institute,
• Place / Publishing House: [Place of publication not identified] : : MDPI - Multidisciplinary Digital Publishing Institute,, 2016.
• Year of Publication: 2016
• Language: English
• Physical Description: 1 online resource (236 pages)

Cells play significant roles in our day to day life. However, the interactions of cells, the cellular responses of organelles to molecules, and their intracellular behaviour, are still not fully understood. To better understand the physiological interactions among molecules, organelles, and cells, the ensemble measurement of (on average, millions of) cells cannot provide detailed information. However, for example, research concerning the differentiation behaviors of stem cells or the metastatic processes of tumour initiation requires detailed information. Understanding genomic sequence information at a single cell level can promote an understanding of how individual parts of a cell are integrated in time and space to form dynamic cellular processes. The relationship between cellular heterogeneity and signaling pathway regulation may result in an understanding of disease states that can potentially drive therapeutic interventions. Thus single cell analysis (SCA) has been emerging as a powerful method of investigating exciting new insights into genomics, fluxomics, proteomics, and systems biology.

Table of Contents

List of Contributors -- About the Guest Editors -- Preface -- Technologies for Single-Cell Isolation -- Single-Cell Isolation and Gene Analysis: Pitfalls and Possibilities -- Digital Microfluidics for Manipulation and Analysis of a Single Cell -- Numerical Analysis of Hydrodynamic Flow in Microfluidic Biochip for Single-Cell Trapping Application -- Get to Understand More from Single-Cells: Current Studies of Microfluidic-Based Techniques for Single-Cell Analysis -- Single Cell Electrical Characterization Techniques -- Microfluidic Impedance Flow Cytometry Enabling High-Throughput Single-Cell Electrical Property Characterization -- Detecting Antigen-Specific T Cell Responses: From Bulk Populations to Single Cells -- Evidence for P-Glycoprotein Involvement in Cell Volume Regulation Using Coulter Sizing in Flow Cytometry -- A Review of Cell Adhesion Studies for Biomedical and Biological Applications.