Lectins : Methods and Protocols /

Lectins : Methods and Protocols / / edited by Jun Hirabayashi.

Lectins: Methods and Protocols summarizes classic lectin technologies and advanced techniques with high throughputs and sensitivities. Chapters include methods and techniques for serial lectin-affinity chromatography procedure, lectin-probed western blot and histochemical analyses, quantitative inte...

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Bibliographic Details
Superior document:Methods in Molecular Biology, 1200
TeilnehmendeR:
Hirabayashi, Jun.
Springer Science+Business Media,
Year of Publication:2014
Language:English
Series:Methods in Molecular Biology, 1200.
Physical Description:1 online resource
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Table of Contents:
  • History of Plant Lectin Research
  • Fungal Lectins a Growing Family
  • The “White Kidney Bean Incident” in Japan
  • Hemagglutination (Inhibition) Assay
  • Preparation of Affinity Adsorbents and Purification of Lectins from Natural Sources
  • High-Performance Lectin Affinity Chromatography
  • Determination of Glycan Motifs Using Serial Lectin Affinity Chromatography
  • Lectin-Probed Western Blot Analysis
  • Solid-Phase Assay of Lectin Activity Using HRP-conjugated Glycoproteins
  • Sialidase-dependent Binding Assay for Influenza and Related Viruses
  • Lectin Affinity Electrophoresis
  • Capillary-Based Lectin Affinity Electrophoresis for Interaction Analysis between Lectins and Glycans
  • Basic Procedures for Lectin Flow Cytometry
  • Histochemical Staining Using Lectin Probes
  • Equilibrium Dialysis using Chromophoric Sugar Derivatives
  • Centrifugal Ultrafiltration-HPLC Method for Interaction Analysis between Lectins and Sugars
  • Surface Plasmon Resonance as a Tool to Characterize Lectin-carbohydrate Interactions
  • Isothermal Calorimetric Analysis of Lectin–sugar Interaction
  • Carbohydrate–Lectin Interaction Assay by Fluorescence Correlation Spectroscopy Using Fluorescence-Labeled Glycosylasparagines
  • Lectin-Based Glycomics: How and When Was the Technology Born?
  • Frontal Affinity Chromatography (FAC): Theory and Basic Aspects
  • Advanced FAC with a Library of Fluorescent-Labeled Oligosaccharides
  • Differential Glycan Analysis of an Endogenous Glycoprotein: Toward Clinical Implementation. From Sample Pretreatment to Data Standardization
  • Lectin-Microarray Technique for Glycomic Profiling of Fungal Cell Surfaces
  • Application of Lectin Microarray to Bacteria Including Lactobacillus casei Shirota
  • Live C.

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