Biochemistry laboratory manual for undergraduates : : an inquiry-based approach / / Timea Gerczei, Scott Pattison; managing editor, Anna Rulka.
Biochemistry laboratory manual for undergraduates - an inquiry based approach by Gerczei and Pattison is the first textbook on the market that uses a highly relevant model, antibiotic resistance, to teach seminal topics of biochemistry and molecular biology while incorporating the blossoming field o...
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| Place / Publishing House: | Warsaw, [Poland] ;, Berlin, [Germany] : : De Gruyter Open,, 2014. ©2014 |
| Year of Publication: | 2014 |
| Edition: | 1st ed. |
| Language: | English |
| Physical Description: | 1 online resource (186 pages) :; illustrations, (some colour) |
| Notes: | Bibliographic Level Mode of Issuance: Monograph |
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Table of Contents:
- Front matter
- Preface
- Contents
- 1 Introducing the Bacterial Antibiotic Sensor Mini Project
- 2 Identifying Conserved Elements in the Toxin Sensor and Designing Mutants to Test Whether They are Important for Function
- 3 Designing Primers for Site-Directed Mutagenesis
- 4 Performing Site-Directed Mutagenesis
- 5 Purifying Mutant Toxin Sensor DNA from Bacterial Cells and Evaluating its Quality Using Agarose Gel Electrophoresis and UV Spectroscopy
- 6 Preparing DNA Template for Mutant RNA Sensor Synthesis Using a Restriction Endonuclease
- 7 Synthesizing the ykkCD Mutant Toxin Sensor RNA in vitro
- 8 Purifying the ykkCD Mutant Toxin Sensor RNA and Evaluating its Purity Using Denaturing PAGE and UV spectrometry
- 9 Evaluating the Ability of the ykkCD Toxin Sensor to Recognize the Antibiotic Tetracycline Using Fluorescent Quenching
- 10 Evaluating Antibiotic Binding to Blood Serum Albumin Using Fluorescence Spectroscopy
- 11 Understanding the Importance of Buffers in Biological Systems
- 12 Molecular Visualization of an Enzyme, Acetylcholinesterase
- 13 Determining the Efficiency of the Enzyme Acetylcholine Esterase Using Steady-State Kinetic Experiment
- 14 Separation of the Phosphatidylcholines Using Reverse Phase HPLC
- List of Figures