Genexpressionsmuster in Thrombozyten und Thrombozyten-Vorläuferzellen / eingereicht von Sandra Rieger
ger: Background: The hemostatic system is of essential importance and platelets (PLT), formed by their precursors (megakaryocytes; MEG) in bone marrow are central components for the maintenance of the vascular integrity. In this study mRNA quantities of PLT specific genes (GPIb, GPIIla, P-selectin,...
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Place / Publishing House: | 2002 |
Year of Publication: | 2002 |
Language: | German |
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Classification: | 42.20 - Genetik |
Physical Description: | 122 Bl.; Ill., graph. Darst. |
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Rieger, Sandra aut Genexpressionsmuster in Thrombozyten und Thrombozyten-Vorläuferzellen eingereicht von Sandra Rieger mRNA expression in human megakaryocytes and platelets 2002 122 Bl. Ill., graph. Darst. Abweichender Titel laut Übersetzung der Verfasserin/des Verfassers Salzburg, Univ., Diss., 2002 ger: Background: The hemostatic system is of essential importance and platelets (PLT), formed by their precursors (megakaryocytes; MEG) in bone marrow are central components for the maintenance of the vascular integrity. In this study mRNA quantities of PLT specific genes (GPIb, GPIIla, P-selectin, vWF) were measured during differentiation of MEG in tissue culture, in PLT of healthy controls and clonal PLT of patients with essential thrombocythemia (ET; 30% thrombosis). Furthermore, we investigated gene transcripts which are up- or downregulated in clonal versus polyclonal PLT population by differential display analysis (DD).<br />We evaluated the association of two frequent polymorphisms in protein S and P-selectin gene with clonal PLT.<br />Patients and methods: Tissue cultures were performed in human megakaryoblastic cells (MEG-01), with and without phorbolester (TPA).<br />Unstimulated and TPA treated cells were isolated on day 0,2,4,6 and 8, used for mRNA extraction and eDNA synthesis. MEG-O1 cells and polyclonal and clonal PLT of 20 healthy controls and 12 ET patients were analysed with Real-time PCR system (LightCyclerTM) to quantify expression of PLT specific genes. With DD eDNA fingerprints of clonal versus polyclonal PLT were compared. The prevalence of the Pro626Pro polymorphism in protein S and the Pro715Thr polymorphism in P-selectin gene was determined in 80 patients with ET and 80 healthy controls. The polymorphisms were then used to study allelic expression of these genes.<br />Results: MEG-01 cells showed significant increase of mRNA following TPA treatment (P-selectin 30x; vWF 20x, GPIb 2x) to a maximum on day 8.<br />GPIIIa mRNA could already be detected on day 2 in 10fold increased concentration. In ET patients we observed a 100times increased expression of vWF and GPIIIa mENA compared to healthy controls. By DD we identified an increased mRNA expression of CD55 in a patient with clonal PLT. Quantification of CD55 mRNA showed elevated mRNA levels only in few ET patients. Polymorphisms in protein S and P-selectin gene were not associated with ET. Allelic expression analysis of protein S and P-selectin showed expression of both alleles in clonal PLT which indicates transcription of both alleles in every MEG. Blutgerinnung s (DE-588)4007275-7 Thrombozyt s (DE-588)4059964-4 Genexpression s (DE-588)4020136-3 AT-OBV UBSRIC YWOAW MAG1-3 30028-C.Stip. 2214523940004498 |
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Rieger, Sandra |
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Rieger, Sandra Genexpressionsmuster in Thrombozyten und Thrombozyten-Vorläuferzellen Blutgerinnung (DE-588)4007275-7 Thrombozyt (DE-588)4059964-4 Genexpression (DE-588)4020136-3 |
author_facet |
Rieger, Sandra |
author_variant |
s r sr |
author_role |
VerfasserIn |
author_sort |
Rieger, Sandra |
title |
Genexpressionsmuster in Thrombozyten und Thrombozyten-Vorläuferzellen |
title_full |
Genexpressionsmuster in Thrombozyten und Thrombozyten-Vorläuferzellen eingereicht von Sandra Rieger |
title_fullStr |
Genexpressionsmuster in Thrombozyten und Thrombozyten-Vorläuferzellen eingereicht von Sandra Rieger |
title_full_unstemmed |
Genexpressionsmuster in Thrombozyten und Thrombozyten-Vorläuferzellen eingereicht von Sandra Rieger |
title_auth |
Genexpressionsmuster in Thrombozyten und Thrombozyten-Vorläuferzellen |
title_alt |
mRNA expression in human megakaryocytes and platelets |
title_new |
Genexpressionsmuster in Thrombozyten und Thrombozyten-Vorläuferzellen |
title_sort |
genexpressionsmuster in thrombozyten und thrombozyten-vorläuferzellen |
publishDate |
2002 |
physical |
122 Bl. Ill., graph. Darst. |
callnumber-raw |
30028-C.Stip. |
callnumber-search |
30028-C.Stip. |
topic |
Blutgerinnung (DE-588)4007275-7 Thrombozyt (DE-588)4059964-4 Genexpression (DE-588)4020136-3 |
topic_facet |
Blutgerinnung Thrombozyt Genexpression |
illustrated |
Illustrated |
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AT riegersandra genexpressionsmusterinthrombozytenundthrombozytenvorlauferzellen AT riegersandra mrnaexpressioninhumanmegakaryocytesandplatelets |
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