Electrophoresis '79 : : Advanced methods, biochemical and clinical applications. Proceedings of the Second International Conference on Electrophoresis, Munich, Germany, October 15–17, 1979 / / ed. by Bertold J. Radola, 1979, München› International Conference on Electrophoresis ‹2.

• Superior document: Title is part of eBook package: De Gruyter DGBA Reference Works <1990
• MitwirkendeR: Agarwal, D. P.,; Aguzzi, F.,; Aitchison, G.F.,; Allen, R.C.,; Allen, Robert C.,; Altland, K.,; Anderson, N. G.,; Anderson, N. L.,; Arnaud, P.,; Arnaud, Philippe,; Baldesten, A.,; Basset, P.,; Benzerara, O.,; Bjerrum, O.J.,; Bleyl, H.,; Bode, H.J.,; Bouissou, C.,; Bours, J.,; Braes, A.V.,; Breslow, Jan L.,; Bronson, P.M.,; Brown, M.P.,; Bruchelt, G.,; Brunner, G.,; Bøg-Hansen, T.C.,; Campens, M.,; Catsimpoolas, N.,; Catsimpoolas, Nicholas,; Cellier, C. Chapuis,; Chapuis-Cellier, C.,; Charles, D.,; Chrambach, A.,; Clerc, A.,; Cloppet, H.,; Constans, J.,; Cook, R.B.,; Coulter, B. P.,; Coulter, B.,; Cunningham, C.G.,; Czempiel, W.,; Delincee, H.,; Drzeniek, R.,; Edwards, J. J.,; Everaerts, F.M.,; Feicht, E.,; Felgenhauer, K.,; Francina, A.,; Franzen, B.,; Froissart, C.,; Fudenberg, H.H.,; Gaal, A.B.,; Garbers, M.,; Gianazza, E.,; Gianazza, Elisabetta,; Giometti, C. S.,; Goedde, H. W.,; Goerth, K.,; Gonzalez-Lama, Z.,; Good, R. A.,; Gouaillard, C.,; Granstrand, B.,; Griffith, A. L.,; Griffith, Ann L.,; Gupta, S.,; Görg, A.,; Hackler, R.,; Haeger, I.,; Hahn, H. J.,; Hallett, D.,; Hamann, A.,; Hampson, F.,; Harada, S.,; Haugen, H.F.,; Hedlund, K. W.,; Henning, R.,; Hickman, B. J.,; Hilbrig, M.,; Hjelmeland, L.,; Hockwin, O.,; Holloway, C.J.,; Husmann-Holloway, S.,; Jayakar, S. D.,; Jellum, E.,; Jeppsson, J.O.,; Just Svendsen, P.,; Kade, W.,; Kasicka, V.,; Klose, J.,; Koussoulakos, S.,; Kruppa, J.,; Köst, H.-P.,; Köst-Reyes, E.,; Lamas, A.M.,; Lambin, Patrick,; Lasne, F.,; Lasne, Y.,; Leaback, D.H.,; Lorenc-Kubis, Irena,; Marasco, P.V.,; Markotai, J.,; Martin, O.H.W.,; Massarelli, R.,; Mattock, P.,; Merlini, G.,; Mikkers, F.E.P.,; Mitzkat, H.-J.,; Müller-Beißenhirtz, W.,; Münch, T.,; Nance, S. L.,; Neuhoff, V.,; Neujahr, H.Y.,; Nguyen, N. Y.,; Nichelson, D.,; Nowak, J.,; Orge, R.H. Lopez,; Oss, C. J. van,; Palme, K.,; Parwaresch, M. R.,; Peichl, H.,; Perolini, S.,; Perry, G.W.,; Perthen, B.,; Peter, R.,; Petrini, C.,; Platsoucas, C. D.,; Postel, W.,; Pozzi, F.,; Pretsch, W.,; Prusik, Z.,; Radola, B. J.,; Radola, B.J.,; Radzun, H. J.,; Recasens, M.,; Reichel, C.,; Righetti, P.G.,; Righetti, Pier Giorgio,; Rosén, Anders,; Rücker, W.,; Saravis, C.A.,; Scandora, A. E.,; Scandora, A.,; Schafer-Nielsen, C.,; Schlanstedt-Jahn, U.,; Schmidt, D.,; Schmidt, K.,; Schroeck, R.,; Simmons, M.A.,; Skoog, B.,; Spengler, G.A.,; Stamatopoulou, Anna,; Stastny, M.,; Stathakos, D.,; Stegemann, H.,; Stein, V.,; Stepánek, J.,; Stone, George C.,; Svendsen, P. Just,; Söderlindh, A.,; Taylor, J.,; Temkin, R.,; Thomson, A.R.,; Thorsrud, A.K.,; Thymann, Mariann,; Tollaksen, S. L.,; Trautschold, I.,; Ulbrich, B.,; Vellios, A.,; Verheggen, Th.P.E.M.,; Vesterberg, O.,; Viau, M.,; Vincendon, G.,; Weber, R.-M.,; Welinder, Karen G.,; Westermeier, R.,; Wiegers, K.J.,; Willard, K. E.,; Wilson, David L.,; Wistar, R.,; Wittke, J. W.,; Zamcheck, N.,; Zannis, Vassiiis I.,; Zwiller, J.,
• HerausgeberIn: International Conference on Electrophoresis ‹2, 1979, München›,; Radola, Bertold J.,
• Place / Publishing House: Berlin ;, Boston : : De Gruyter, , [2019]; ©1980
• Year of Publication: 2019
• Edition: Reprint 2019
• Language: English
• Physical Description: 1 online resource (858 p.) :; Num. figs.

Table of Contents

Frontmatter -- PREFACE -- CONTENTS -- SECTION I. THEORY AND METHODS -- GEL ELECTROFOCUSING WITH INCREASED DEGREES OF FREEDOM -- pH-MOBILITY CURVES OF PROTEINS BY ISOELECTRIC FOCUSING COMBINED WITH ELECTROPHORESIS AT RIGHT ANGLES -- PARTITIONING AND ELECTROPHORESIS IN FLEXIBLE POLYMER NETWORKS -- DOUBLE ONE-DIMENSIONAL SLAB-GEL ELECTROPHORESIS -- ULTRATHIN-LAYER HORIZONTAL ELECTROPHORESIS, ISOELECTRIC FOCUSING, AND PROTEIN MAPPING IN POLYACRYLAMIDE GELS ON CELLOPHANE -- ULTRATHIN-LAYER ISOELECTRIC FOCUSING IN 50-100 pm POLYACRYLAMIDE GELS ON SILANIZED GLASS PLATES OR POLYESTER FILMS -- ISOELECTRIC FOCUSING OF PROTEINS IN AGAROSE GELS AND DETECTION OF THE PROTEINS BY DIFFERENT STAINING PROCEDURES -- AGAROSE ISOELECTRIC FOCUSING OF MONOCLONAL IgM AND IgG ANTIBODIES -- IMMUNOPEROXIDASE STAINING AND RADIOIMMUNOBINDING OF HUMAN TUMOR MARKERS SEPARATED BY DIRECT TISSUE AGAROSE ISOELECTRIC FOCUSING -- AGAROSE AS SUPPORTING MEDIUM FOR ANALYTICAL ISOELECTRIC SING. DETERMINATION OF THE RECENTLY DISCOVERED SUBTYPES HUMAN SERUM PROTEIN Gc (GROUP SPECIFIC COMPONENT) -- FACTS AND ARTEFACTS IN ISOELECTRIC FOCUSING -- ISOELECTRIC FOCUSING PATTERNS OF SIMIAN VIRUS 40 (SV 40) TUMOR ANTIGEN: VARIOUS ALKYLATION PROCEDURES CAUSE REVERSIBLE ARTIFACTS -- THE ROLE OF SIALIC ACIDS IN THE MICROHETEROGENEITY OF ALPHA ACID GLYCOPROTEIN : STUDY BY ISOELECTRIC FOCUSING AND TITRATION CURVES -- THE EFFECT OF ELECTRODE SOLUTIONS ON THE pH DRIFT IN THIN-LAYER ISOELECTRIC FOCUSING -- QUANTITATIVE IMMUNOELECTROPHORESIS. A SURVEY AND SOME APPLICATIONS. -- AFFINITY ELECTROPHORESIS. QUANTIFICATION AND CHARACTERIZATION OF GLYCOPROTEINS WITH LECTINS -- RECENT ADVANCES IN MICROELECTROPHORESIS -- A SIMPLE, EFFECTIVE AND INEXPENSIVE EQUIPMENT FOR POLYACRYLAMIDE GEL ELECTROPHORESIS IN COOLED MICROCAPILLARIES -- LINEAR POLYACRYLAMIDE GRADIENT GELS FOR MOLECULAR WEIGHT ESTIMATION OF PROTEINS -- SOLUBLE POLYACRYLAMIDE GRADIENT GELS IN THE SEPARATION AND ESTIMATION OF PROTEINS -- QUANTITATION OF PROTEINS SEPARATED BY TWO-DIMENSIONAL PAGE: A NOVEL DYE ELUTION TECHNIQUE COMPARED WITH A DENSITOMETRIC PROCEDURE -- PREPARATION AND ANALYSIS OF PROTEINS AND PROTEIN FRAGMENTS BY TWO-DIMENSIONAL THIN-LAYER GEL FILTRATION AND ISOELECTRIC FOCUSING -- ENHANCEMENT OF LOAD CAPACITY IN ISOTACHOPHORESIS -- A MODEL FOR THE USE OF MORE THAN ONE SINGLE BUFFERING COMMON COUNTERION IN ISOTACHOPHORESIS -- SEPARATION OF MOLECULES IN STEADY STATE ELECTROPHORESIS SYSTEMS WITH ZONES CONTAINING MORE THAN ONE TERMINATING ION -- FLOW-THROUGH CONTINUOUS THIN LAYER CARRIER-FREE ISOTACHOPHORESIS -- SECTION II. HIGH RESOLUTION TWO-DIMENSIONAL ELECTROPHORESIS -- TWO-DIMENSIONAL ELECTROPHORESIS: HIGH RESOLUTION OF PROTEINS AND AUTOMATIC EVALUATION OF THE PATTERNS UNDER DIFFERENT METHODICAL CONDITIONS -- HIGH-RESOLUTION TWO-DIMENSIONAL ELECTROPHORETIC MAPPING OF HUMAN PROTEINS -- ESTIMATION OF TWO-DIMENSIONAL ELECTROPHORETIC SPOT INTENSITIES AND POSITIONS BY MODELING -- INTERNAL CHARGE STANDARDIZATION FOR TWO-DIMENSIONAL ELECTROPHORESIS -- A METHOD FOR STUDYING PROTEINS IN 2-D GELS USING THERMAL DENATURATION ANALYSIS -- THE QUANTITATION OF RADIOACTIVELY LABELED PROTEINS ON TWO-DIMENSIONAL GELS: TESTS OF A METHOD FOR ANALYZING CHANGES IN PROTEIN SYNTHESIS AND GENE EXPRESSION -- TWO-DIMENSIONAL ELECTROPHORETIC MAPPING OF HUMAN ERYTHROCYTE PROTEINS -- TWO-DIMENSIONAL ELECTROPHORESIS OF HUMAN SALIVA -- TWO-DIMENSIONAL ELECTROPHORESIS OF HUMAN URINARY PROTEINS IN HEALTH AND DISEASE -- ALTERATIONS OF TWO-DIMENSIONAL ELECTROPHORETIC MAPS IN HUMAN PERIPHERAL BLOOD LYMPHOCYTES INDUCED BY CONCANAVALIN A -- HIGH RESOLUTION TWO-DIMENSIONAL ELECTROPHORESIS (ISO-DALT) IN CLINICAL CHEMISTRY: COMPARISON WITH ROUTINE ELECTROPHORETIC AND IMMUNOLOGICAL METHODS -- TWO DIMENSIONAL MAPS OF HUMAN APOLIPOPROTEINS IN NORMAL AND DISEASED STATES -- ISOELECTRIC FOCUSING AND TWO-DIMENSIONAL ELECTROPHORESIS OF VIRAL PROTEINS AND VIRUS-INFECTED CELLS -- SECTION III. PREPARATIVE SEPARATIONS -- ISOELECTRIC FOCUSING, ISOELECTRIC POINTS, AND THE PREPARATIVE SEPARATION OF PROTEINS -- PREPARATIVE MULTIPHASIC ZONE ELECTROPHORESIS AND ISOELECTRIC FOCUSING IN DENSITY GRADIENTS USING THE POLY-PREP -- MULTIPHASIC COLUMN ELECTROFOCUSING: AN APPROACH TO COMPOSITE FRACTIONATION PROBLEMS -- PREPARATIVE ELECTROFOCUSING OF ENZYMES INVOLVED IN AROMATIC DEGRADATION IN T. CUTANEUM -- PREPARATIVE FLAT-BED ISOELECTRIC FOCUSING OF LDH, MDH, ALD, SDH AND G-6.PDH FROM RABBIT LENS -- CONTINUOUS-FLOW ISOELECTRIC FOCUSING OF PROTEINS -- RECYCLING OF CARRIER AMPHOLYTES BY ULTRAFILTRATION -- REMOVAL OF SALTS AND CARRIER AMPHOLYTES FROM BILIPEPTIDES OF LOW MOLECULAR WEIGHT -- PREPARATIVE ELECTROPHORESIS IN GEL-BLOCKS WITH DISCONTINUOUS ELUTION -- FURTHER DEVELOPMENTS IN PREPARATIVE DISPLACEMENT ELECTROPHORESIS IN GEL -- VELOCITY GRADIENT STABILISED CONTINUOUS ELECTROPHORESIS -- PREPARATIVE DENSITY GRADIENT ELECTROPHORESIS OF LYMPHOID CELLS -- PREPARATIVE CELL ELECTROPHORESIS IN A STATIC VERTICAL COLUMN STABILIZED WITH A HEAVY WATER GRADIENT -- SECTION IV. BIOMEDICAL AND BIOLOGICAL APPLICATIONS -- PAST, PRESENT, AND FUTURE USES OF ELECTROPHORESIS IN CLINICAL MEDICINE -- TWO-DIMENSIONAL PROTEIN PATTERNS OF HUMAN BODY FLUIDS OBTAINED UNDER DISSOCIATING AND NON-DISSOCIATING CONDITIONS -- SIMPLIFIED DETERMINATION OF HEMOGLOBIN A1c DIABETIC PATIENTS BY USE OF ELECTROFOCUSING -- A COMPARISON OF ISOELECTRIC FOCUSING AND ELECTROCHROMATOGRAPHY FOR THE SEPARATION AND QUANTIFICATION OF HEMOGLOBIN A1c -- INVESTIGATIONS OF HAEMOGLOBIN A1c ANALOGUES BY ELECTROFOCUSING ON POLYACRYLAMIDE GELS -- ISOELECTRIC ENZYME PATTERN : A NEW CONCEPT FOR TUMOUR CLASSIFICATION -- MONOCYTE-SPECIFIC ISOELECTRIC FOCUSING PATTERN OF LYSOSOMAL ACID ESTERASE : A NEW POSSIBILITY FOR CELL-SPECIFIC IMMUNOHISTOCHEMICAL IDENTIFICATION -- ISOELECTRIC FOCUSING IN CELLULOSE ACETATE MEMBRANE AND FLATBED GRANULATED GEL: APPLICATION TO THE STUDY OF HUMAN ALDEHYDE DEHYDROGENASE ISOZYMES -- CEREBROSPINAL FLUID (CSF) PROTEIN PATTERNS DETERMINED BY ISOELECTRIC FOCUSING IN MULTIPLE SCLEROSIS -- BINDING AFFINITIES BETWEEN VDBP AND VITAMIN D3, 25-(OH)-D3, 24-25-(OH)2-D3 and 1-25-(OH)2-D3 STUDIED BY ELECTROPHORETIC METHODS (PAGE-IE-F, COMBINED IEFELECTROPHORESIS) AND PRINT-IMMUNOFIXATION -- IMMUNOFIXATION AFTER ELECTROFOCUSING : APPLICATION TO THE STUDY OF THE MICROHETEROGENEITY AND POLYMORPHISM OF SERUM PROTEINS -- MICROHETEROGENEITY AND POLYMORPHISM OF THYROXINE-BINDING GLOBULIN: STUDY IN HUMAN SERUM BY RADIOPRINT IMMUNOFIXATION -- TWO DIMENSIONAL IMMUNELECTROPHORESIS OF PLASMA-ANTITHROMBIN III IN THE PRESENCE OF HEPARIN-AGAROSE -- DETERMINATION OF STREPTOKINASE ANTIBODY TITRE IN PLASMA OR SERUM BY AN IMMUNOELECTROPHORETIC METHOD -- TWO DIMENSIONAL IMMÜNOELECTPOPHORESIS APPLIED TO THE SEMI-QUANTITATIVE DETERMINATION OF BENCE JONES IN PROTEIN IN PATHOLOGICAL URINES -- APPLICATION OP PHA IN ELECTROPHORETIC DIFFERENTIATION OF HUMORAL IMMUNOGLOBULINS -- FEASIBILITY OF STUDYING THE FUNCTIONAL NATURE OF HUMAN IgG SUBCLASS RESPONSES BY MEANS OF ANALYTICAL ISOTACHOPHORESIS -- IN VITRO INVESTIGATIONS OF THE GLUCURONIC ACID PATHWAY WITH THE AID OF ANALYTICAL CAPILLARY ISOTACHOPHORESIS -- QUANTITATIVE RESOLUTION OF 1- AND 2-NAPHTHYLGLUCURONIDES FROM SINGLE MIXED ZONES IN ISOTACHOPHEROGRAMS -- A RAPID METHOD FOR QUANTITATIVE DETERMINATION OF URINARY OXALATE BY ANALYTICAL ISOTACHOPHORESIS -- STUDIES ON SERUM CHOLINESTERASE ISOZYMES AND GENETIC VARIANTS IN HUMAN TISSUES USING POLYACRYLAMIDE GEL ELECTROPHORESIS -- MATERNAL PLASMA PROTEIN VARIATIONS DURING NORMAL AND PATHOLOGIC PREGNANCY: A COMPARATIVE STUDY BY MEANS OF SDS-PAA ELECTROPHORESIS AND OSMOMETRIC EVALUATION OF NUMBER AVERAGE MOLECULAR WEIGHT -- SERUM PROTEIN ANALYSIS BY MOLECULAR INTERPRETATION OF CELLULOSE ACETATE ELECTROPHORESIS: EVALUATION OF ITS CLINICAL USEFULNESS -- GENETICAL AND BIOCHEMICAL CHARACTERIZATION OF A DOMINANT MUTATION OF MOUSE LACTATE DEHYDROGENASE -- VERTEBRATE MITOCHONDRIAL 55S RIBOSOMES: COMPARISON OF PROTEIN PATTERNS WITH BACTERIAL AND CYTOPLASMIC R-PROTEINS BY 2-D PAGE -- ISOELECTRIC PEROXIDASE PATTERNS.AS INDICATORS FOR GROWTH AND FOR THE EFFECT OF GROWTH REGULATORS IN TOBACCO TISSUE CULTURES -- STUDY OF HEMOGLOBIN AND A SOLUBLE HEART PEROXIDASE IN LACERTA GALLOTI AND LACERTA STHELINI BY ISOELECTRIC FOCUSING -- AUTHOR INDEX -- SUBJECT INDEX -- Backmatter