Electrophoresis ‘82 : : Advanced methods, biochemical and clinical applications. Proceedings of the [4th] International Conference on Electrophoresis, Athens, Greece, April, 21– 24, 1982 / / ed. by Dimitri Stathakos, Electrophoresis Society, 1982, Athenai› International Conference on Electrophoresis ‹4.

• Superior document: Title is part of eBook package: De Gruyter DGBA Reference Works <1990
• MitwirkendeR: Abbadi, M.-C.,; Abe, M.,; Acton, R.T.,; Ageshio, M.,; Albrechtsen, J.,; Allen, Robert C.,; Andrews, Brenda J.,; Ansorge, W.,; Avgerinos, A.,; Bag-Hansen, T. C.,; Baldo, B.A.,; Bardou, Louis Georges,; Barger, B.O.,; Bari, Mario,; Barker, R.,; Barnhoorn, M.G.,; Bartels, Rainer,; Ben-Or, Sarah,; Bier, M.,; Bjellqvist, Bengt,; Blenke, H.,; Bock, Lothar,; Bosisio, Adriana Bianchi,; Bouchahda, A.,; Bours, J.,; Breborowicz, J.,; Brodersen, H.-P.,; Brooks, K.P.,; Brosstad, Frank,; Budowle, B.,; Burghes, A.H.M.,; Bøg-Hansen, T.C.,; Campbell, W.P.,; Castell, J. V.,; Chen, John S.,; Chrambach, Andreas,; Clark, P. M. S.,; Cleve, H.,; Colorna, J.,; Constans, J.,; Cook, Richard B.,; Cornell, F.N.,; Coucke, P.,; Coucoli, E.,; Coulson, Susan E.,; Cox, Diane Wilson,; Cros, D.L. du,; Cutillas, M.J.,; Czikeli, H.,; Das, Joachim,; Diesfeld, Hans-Jochen,; Ditter, Bernhard,; Dubowitz, V.,; Dunn, M.J.,; Ebert, Michael H.,; Ek, Kristina,; El-Zawahri, Mokhtar,; Everaerts, Frans,; Figueroa, F.,; Fujimoto, Masanori,; Fônagy, Anna,; Galle-De Ville, Y.,; Garbers, M.,; Geada, Helena,; Gelfi, Cecilia,; Gemeiner, M.,; Georgiev, G.,; Ghaffor, M.,; Gianazza, Elisabetta,; Gibson, Sharon G.,; Gillis, E.,; Gillis-Van Maele, A.,; Go, R.C.P.,; Goldman, David,; González-Lama, Z.,; Gonzälez-Lama, Z.,; Gordon, Julian,; Graham, A.,; Groenier, K.,; Görg, Angelika,; Hannig, Kurt,; Hansen, Ernil,; Hare, R.A.,; Hashimoto, N.,; Hau, J.,; Havsteen, B.,; Heiland, Randi,; Hennekes, H.,; Henning, R.,; Henriksson, K.-G.,; Hidvégi, Egon J.,; Hinnerfeldt, F.R.,; Hjerten, Stellan,; Hockwin, O.,; Horhammer, Werner,; Horita, M.,; Hussein, Younis,; Iaminarino, R.M.,; Institoris, Laszlo,; Izumi, M.,; Jeney, Andras,; Jeppsson, J.-O.,; Kalantzis, N.,; Kanaghinis, T.,; Kantcheva, Maria,; Kessler, Reinhard,; Keuren, Margaret L. Van,; Klein, H.,; Klingenberg, M.,; Kobayashi, T.,; Kolin, Alexander,; Kostulas, V.K.,; Kouvatsi, A.,; Kreckel, P.,; Kreuzer, H.,; Kronberg, Harald,; Kuwahara, Akira,; Köst, Hans-Peter,; Kühnl, P.,; Laas, Torgny,; Lab, M.,; Lamas, A.M.,; Lan, Birgit C. An der,; Larsen, P.,; Lemaire, G.,; Lenger, Karin,; Lescoat, Gérard,; Link, H.,; Liu, Zhao-qian,; Lossnitzer, K.,; Lucius, Richard,; Léglise, Dominique,; López-Orge, R.H.,; Manchester, Keith,; Manso, Carlos,; Margolis, J.,; Matsumoto, H.,; Maudelonde, Thierry,; McLachlan, R.,; Meléndez-Hevia, E.,; Menesini Chen, Maria Giulia,; Menez, Jean François,; Merril, C.R.,; Merril, Carl R.,; Miller, I.,; Mosher, R.A.,; Moustakas, M.,; Nestruck, A.C.,; Neuhoff, Volker,; Niedmann, P.D.,; Nielsen, Peter,; Nilsson, Marianne,; Noben, J.P.,; Nochumson, Samuel,; Nose, S.,; Olaisen, Bjørnar,; Olsson, Ingmar,; Olsson, T.,; Paleos, C.M.,; Palme, K.,; Palusinski, O.A.,; Petit, J.-F.,; Platsoucas, Chris D.,; Polony, G.,; Pompucci, Giuseppe,; Ponteur-Van Stichel, A.,; Popdimitrova, Nezabravka,; Portocala, R.,; Postel, Wilhelm,; Poucke, M. Van,; Power, G. M.,; Rabhi, H.,; Raff, M.,; Rauterberg, Ernst-Wilhard,; Reijenga, Jetse,; Rekoumis, G.,; Ricci, Carlo,; Righetti, Pier Giorgio,; Roseman, J.M.,; Rothe, Gunter M.,; Sack, K.,; Saville, D.A.,; Sbardellati, Elio,; Schafer-Nielsen, C.,; Schafer-Nielsen, Claus,; Schmidt, Ahlert,; Schmidt, K.G.,; Schultheiss, H.-P.,; Schwarzfischer, F.,; Seidel, D.,; Shibata, K.,; Sierra, A.,; Simonsen, Vibeke,; Soteriadou, K.,; Spielmann, W.,; Spyrou, N.,; Stathakos, D.,; Statham, H.E.,; Stoylov, Stoyl,; Stroll, E.,; Sullivan, J. V.,; Sullivan, James V.,; Sutton, R.,; Suzuki, K.,; Suzuno, Ryosuke,; Svendsen, P. Just,; Svendsen, Per Just,; Szamos, Jeno,; Takeo, K.,; Takeo, Kazusuke,; Taketa, K.,; Tanaka, Tatehiko,; Teige, Brita,; Teisner, B.,; Thoma, Hedwig,; Thomas, George,; Thompson, B.J.,; Timmerman, A.,; Toguchi, E.,; Tornqvist, H.,; Towbin, Harry,; Triantaphyllidis, C.,; Tsekos, I.,; Tulp, A.,; Urbaschek, Bernhard,; Urbaschek, Renate,; Valcke, R.,; Vellios, A.,; Verheggen, Theo,; Viau, M.,; Vienne, Dominique de,; Wagner, Horst,; Warm, Erich,; Weidinger, S.,; Westerbrink, K.,; Westermeier, Rainer,; Whitehead, T. P.,; Wiegand, V.,; Wieland, H.,; Witkowski, J.A.,; Wrigley, C.W.,; Wurster, Ulrich,; Yakin, Habib Mohammed,; Zhao, Su-lian,; Zimmer, Hans-Georg,
• HerausgeberIn: Electrophoresis Society, ,; International Conference on Electrophoresis ‹4, 1982, Athenai›,; Stathakos, Dimitri,
• Place / Publishing House: Berlin ;, Boston : : De Gruyter, , [2019]; ©1983
• Year of Publication: 2019
• Edition: [Held in conjunction with the annual meeting of the Electrophoresis Society]. Reprint 2019
• Language: English
• Physical Description: 1 online resource (867 p.) :; Num. figs.

Table of Contents

Frontmatter -- PREFACE -- CONTENTS -- SECTION I. PLENARY LECTURE -- EVOLUTION OF IDEAS IN ELECTROPHORETIC DEVELOPMENTS - Selected Highlights -- SECTION II. THEORY AND METHODS -- A UNIFIED MATHEMATICAL THEORY OF ELECTROPHORETIC PROCESSES -- ISOELECTRIC FOCUSING IN IMMOBILIZED pH GRADIENTS. I: PRINCIPLE AND METHODOLOGY -- ISOELECTRIC FOCUSING IN IMMOBILIZED pH GRADIENTS. II: APPLICATION TO HEMOGLOBIN ANALYSIS -- COMPUTER SIMULATION OF MODEL ISOELECTRIC FOCUSING EXPERIMENTS -- QUANTITATIVE MODELS FOR THE MIGRATION OF ELECTROLYTE GRADIENTS IN AN ELECTRIC FIELD -- APPLICABILITY OF THE CORRELATION LOG SIZE - SQUARE ROOT OF MIGRATION DISTANCE TO SDS ELECTROPHORESIS IN LINEAR POLYACRYLAMIDE GRADIENT GELS -- VOLTAGE REGULATION ACROSS MULTIPLE GEL TUBES IN ELECIROFOCUSING, USING WEAKLY ACIDIC AND BASIC ANOLYTES AND CATHOLYTES -- A GENERAL METHOD FOR THE STUDY OF SOME PROPERTIES OF POLYMERIC PROTEINS -- HIGHLY CROSS-LINKED GRADIENT GELS FOR DETERMINING MOLECULAR WEIGHT WITH NATIVE PROTEINS OF ALL SIZES -- HIGH RESOLUTION DNA SEQUENCING SYSTEM -- POLYMERIZATION KINETICS OF POLYACRYLAMIDE GELS: EFFECTS OF DIFFERENT CROSSLINKERS, TEMPERATURE AND CATALYSTS -- ISOELECTRIC FOCUSING OF IMMUNOGLOBULINS -- SYNTHETIC AMIDE-TYPE OLIGOMERS AS POTENTIAL CARRIER AMPHOLYTES -- A NEW POLYACRYLAMIDE GEL FORMULATION ALLOWING OVEN DRYING OF HIGH PERCENTAGE SLAB GELS ON GELBONÖ® PAG -- RECOVERY OF PROTEINS FROM GEL SLABS BY A COMBINATION OF DISPLACEMENT ELECTROPHORESIS AND ISOELECTRIC FOCUSING -- AUTOMATIC EVALUATION OF ONE-DIMENSIONAL ELECTROPHEROGRAMS BY REFINED MODELLING -- QUANTITATIVE EVALUATION OF CAF - PATTERN BY VIDEO DENSITOMETRY -- IDENTIFICATION OF FUNCTIONAL PROPERTIES ASSOCIATED WITH INDIVIDUAL PROTEINS BY COMPUTER-BASED PATTERN ANALYSIS -- EVALUATION OF ISOELECTRIC FOCUSING PATTERN BY VIDEO DENSITOMETRY -- EFFECTIVE FIXATION AND RAPID STAINING OF PROTEINS IN SDS-PAGE -- FAST VISUALISATION OF PROTEIN BANDS BY IMPREGNATION IN POTASSIUM PERMANGANATE AND SILVER NITRATE -- SILVER STAIN IMMUNOFIXATION FOR a2HS-GLYC0PR0TEIN: A NEW METHOD FOR DETECTION OF PROTEIN HETEROGENEITY -- DEMONSTRATION OF OLIGOCLONAL IGG IN THE UNCONGENTRATED CSF BY SILVER STAIN -- AFFINITY ELECTROPHORETIC SYSTEMS COMPARED WITH THE CORRESPONDING AFFINITY COLUMN SYSTEMS - GENERAL PRINCIPLES -- DEMONSTRATION AND QUANTIFICATION OF MICROHETEROGENEITY FORKS. LECTIN AFFINITY ELECTROPHORESIS OF HUMAN ɑ-FETOPROTEIN -- STUDIES ON HETEROGENEITY OF ANTI-DNP ANTIBODY BY MEANS OF TWO DIMENSIONAL AFFINITY ELECTROPHORESIS -- DETECTION OF ALLERGENS FOLLOWING GEL ELECTROPHORESIS -- QUALITATIVE CHARACTERISTICS OF CAPILLARY ISOTACHOPHORESIS -- FREE FLOW FIELD STEP FOCUSING - A NEW METHOD FOR PREPARATIVE PROTEIN ISOLATION -- ASECS: ANTIGEN-SPECIFIC ELECTROPHORETIC CELL SEPARATION -- PREPARATIVE DENSITY GRADIENT ELECTROPHORESIS OF CELLS AND CELL ORGANELLES. A NEW SEPARATION CHAMBER -- SECTION III. TWO-DIMENSIONAL SEPARATIONS -- MOLECULAR PROBES FOR HUMAN GENETIC DISEASES BY TWO-DIMENSIONAL PROTEIN ELECTROPHORESIS AND SILVER STAINING -- TWO-DIMENSIONAL ELECTROPHORESIS OF PROTEINS: USE OF CELLULOSE ACETATE MEMBRANE AND ISOELECTRIC FOCUSING GEL -- ISOELECTRIC FOCUSING IN IMMOBILIZED pH GRADIENTS. Ill: APPLICATION TO TWODIMENSIONAL SEPARATIONS -- TWO-DIMENSIONAL ELECTROPHORESIS IN AGAROSE GELS -- ASPECTS OF HIGH RESOLUTION TWO DIMENSIONAL GEL ELECTROPHORESIS : ATTEMPTS TO INCREASE RESOLUTION -- 2D-ELECTROPHORESIS OF PROTEINS IN UNICELLULAR ALGA E. GRACILIS AND YEAST S. UVARUM -- POLYPEPTIDE COMPOSITION OF DIFFERENT CHLOROPLAST FRACTIONS FRON BARLEY ANALYZED BY 1- AND 2-DIMENSIONAL GEL ELECTROPHORESIS -- TWO-DIMENSIONAL ELECTROPHORESIS OF SIMIAN VIRUS 40 (SV40) TUMOR ANTIGEN TWO-DIMENSIONAL ELECTROPHORESIS OF SIMIAN VIRUS 40 (SV40) TUMOR ANTIGEN -- TWO-DIMENSIONAL ELECTROPHORESIS OF FIBRINOGEN AND FIBRIN SUBUNIT CHAINS -- NUMERICAL TAXONOMY OF TWO-DIMENSIONAL PROTEIN MAPS: A RATIONAL BIOCHEMICAL APPROACH TO TUMOR CHARACTERIZATION -- ISO-DALT ANALYSIS OF URINARY PROTEINS IN RENAL FAILURE -- BINDING OF ANTICANCER DIBROMO-DULCITOL AND DIANHYDRO-DULCITOL (GALACTITOL) TO NUCLEAR PROTEINS IN ASCITES TUMOUR CELLS -- A SIMPLE ONE DIMENSIONAL GEL PROCEDURE FOR DETERMINATION OF THE EXTENT OF PHOSPHORYLATION OF RIBOSOMAL PROTEIN S6 -- SECTION IV. BIOLOGICAL AND BIOMEDICAL APPLICATIONS -- COMPARISON OF ACTIVITY AND PROTEIN PROFILES OF ENDOTOXINS EXTRACTED AFTER VARIOUS INTERVALS OF BACTERIAL GROWTH ON SOLID MEDIUM -- POLYACRYLAMIDE GEL ELECTROFOCUSING OF BETA-LACTAMASES FROM PROTEUS MIRABILIS -- POLYACRYLAMIDE-STARCH GEL ELECTROFOCUSING OF CATALASE FROM PSEUDOMONAS AERUGINOSA -- FRACTIONATION OF 7,8-DIHYDROPTEROATE-SYNTHETASE FROM E.COLI BY AFFINITY CHROMATOGRAPHY AND ISOELECTRIC FOCUSING TECHNIQUE -- PURIFICATION OF 7,8-DIHYDROPTERIDINEALCOHOL PYROPHOSPHOKINASE FROM E.COLI AND CHARACTERIZATION BY ULTRATHIN LAYER IEF -- ANALYSIS OF PROTEIN AND POLY-A+ RNA IN NORMAL AND SULFURSTARVED SYNECHOCOCCUS 6301 -- ELECTROPHORETIC MEASUREMENTS Qll PURPLE MEMBRANE PARTICLES -- THE ACTIVITIES OF ISOLATED ALKALINE- AND ACID-DNases ON DENATURED AND NATIVE DNA -- A FUNGAL PROTEINASE RESISTANT PROTEIN IN BOVINE PLASMA -- IDENTIFICATION OF FILARIAL PROTEINS, INDUCING AN IMMUNE RESPONSE IN NATURAL INFECTIONS OF RODENTS WITH DIPETALONEMA VITEAE (FILAROIDEA) BY THE "WESTERN BLOTTING" TECHNIQUE -- AN ECONOMICAL MICROSCALE COUNTER-IMMUNOELECTROPHORESIS METHOD FOR SCREENING OF ALEUTIAN DISEASE IN MINK -- CROSSED IMMUNOELECTROPHORESIS FROM SDS-POLYACRYLAMIDE GELS -- COMPARISON OF WAGTAIL LIVER ISOZYME PATTERNS IN AGAROSE THIN LAYER ISOELECTRIC FOCUSING WITH THOSE OBTAINED BY ISOELECTRIC FOCUSING ON POLYACRYLAMIDE GELS (PAGIF) -- MALATE DEHYDROGENASE ISOENZYMES FROM RABBIT LENS, IRIS AND RETINA -- THE MICROHETEROGENEITY FORMS OF HUMAN SERUM PROTEINS ARE REMARKABLY CONSTANT. A SYSTEMATIC STUDY OF REACTION WITH ConA DURING ELECTROPHORESIS -- AMPLIFICATION OF IMMUNOPRECIPITATES WITH HORSERADISH PEROXIDASE-LABELED PROTEIN A: APPLICATION TO STUDIES OF INTERACTION BETWEEN α-PETROPROTEIN AND LECTINS BY AFFINITY ELECTROPHORESIS -- ANALYSIS OF CENTRIFUGATION GRADIENTS BY FUSED ROCKET IMMUNOELECTROPHORESIS -- QUANTITATIVE IMMUNOELECTROPHORESIS FOR COMPARATIVE ANALYSIS OF HYDROPHOBIC INTRINSIC MEMBRANE PROTEINS -- SENSITIVE MEASUREMENT OF 3H-GLUCOCORTICOID-RECEPTORCOMPLEXES BY GEL-ELECTROPHORESIS -- ELECTROPHORESIS AND ISOELECTRIC FOCUSING OF MAMMALIAN SEMEN PROTEINS -- POLYPEPTIDES SECRETED BY RESIDENT, INFLAMMATORY AND ACTIVATED MACROPHAGES -- DIRECT TISSUE ISOELECTRIC FOCUSING OF NERVOUS SYSTEM AND MUSCLE SECTIONS FOR DETECTION OF IgG PATTERNS -- DETERMINATION OF PREHbA1c BY USE OF ISOELECTRIC FOCUSING -- ELECTROPHORETIC ANALYSIS OF TISSUES IN DUCHENNE MUSCULAR DYSTROPHY -- CHANGES OF MYOSIN ISOENZYMES IN THE CARDIOMYOPATHIC SYRIAN HAMSTER AND IN THE HUMAN MYOCARDIUM -- CHROMATOFOCUSING OF HIGH DENSITY LIPOPROTEINS -- ISOELECTRIC FOCUSING OF PLASMA LIPOPROTEINS PRE-STAINED WITH TETRAZOLIUM BLUE: A PROMISING DIAGNOSTIC TOOL -- A NEW DETAILED LIPIDOGRAM: METHODS AND CLINICAL APPLICATIONS -- THE COURSE OF PROTEINURIA IN GENTAMICIN-TREATED RATS: IEF AND IMMUNOFLUORESCENCE MARKING OF PROTEINS ON THE PAG-PLATE -- AGAROSE ISOELECTRIC FOCUSING OF UNCONCENTRATED CSF AND RADIOIMMUNOFIXATION FOR DETECTION OF OLIGOCLONAL BANDS IN PATIENTS WITH MULTIPLE SCLEROSIS AND OTHER NEUROLOGICAL DISEASES -- PREPARATIVE ISOELECTRIC FOCUSING IN AGAROSE GELS AND ITS APPLICATION IN THE INVESTIGATION OF GAMMOPATHIES -- ROCKET IMMUNOSELECTION USING PEROXIDASE LABELED ANTIBODIES IN THE DIAGNOSIS OF ALPHA HEAVY CHAIN DISEASE -- CLASSICAL ELECTROPHORETIC TECHNIQUES VERSUS ULTRATHINLAYER FOCUSING AS TOOLS IN POPULATION GENETIC STUDIES -- C4 PHENOTYPES IN CAUCASIANS FROM THE SOUTHEASTERN UNITED STATES -- ISOELECTRIC FOCUSING OF SEED PROTEINS FROM NATURAL GREEK POPULATIONS OF TWO AGROPYRON SPECIES -- PLACENTAL ENZYME POLYMORPHISMS IN THE HUMAN POPULATION OF NORTHERN GREECE -- ELASTASE INHIBITION BY SUBTYPES OF THE M GENETIC VARIANT OF α1-ANTITRYPSIN -- ISOELECTRIC POINTS AND TITRATION CURVES OF THE THREE HAPTOGLOBIN PEPTIDE CHAINS α,α2 AND β -- ANTITHROMBIN III: A NEW POLYMORPHISM REVEALED BY ISOELECTROFOCUSING AND IMMUNOFIXATION -- COAGULATION FACTOR XIII B (FXIIIB) ALLOTYPING BY IMMUNOFIXATION ELECTROPHORESIS: CONFIRMATION OF THE THREE-ALLELE-MODEL -- SEPARATION OF LEUKEMIC B CELLS FROM

PATIENTS WITH CHRONIC LYMPHOCYTIC LEUKEMIA BY DENSITY GRADIENT ELECTROPHORESIS. FUNCTIONAL AND PHENOTYPIC HETEROGENEITY OF THE SEPARATED CELL FRACTIONS -- ELECTROPHORETIC MOBILITY TEST BY STIMULATED LYMPHOCYTE SUPERNATANTS -- SECTION V: ROUND-TABLE DISCUSSIONS -- A. ACCURACY AND PRECISION OF 2-DIMENSIONAL MACROMOLECULAR MAPPING -- B. A SILVER STAIN WORKSHOP -- C. PEPTIDE FOCUSING -- AUTHOR INDEX -- SUBJECT INDEX