Methods in Protein Biochemistry / / ed. by Harald Tschesche.

• Superior document: Title is part of eBook package: De Gruyter DGBA Backlist Complete English Language 2000-2014 PART1
• MitwirkendeR: Beranova-Giorgianni, Sarka,; Boisguerin, Prisca,; Byers, David M.,; Caramelo, Julio J.,; Chen, Li,; Corrêa, Ivan R.,; Cronan, John E.,; Deber, Charles M.,; Dennison, Clive,; Desiderio, Dominic M.,; Dreiseidler, Michael,; Fierz, Beat,; Fischetti, Robert F.,; Giorgianni, Francesco,; Glockshuber, Rudi,; Gooptu, Bibek,; Hanek, Ariele,; Herrmann, Johannes M.,; Hirano, Hisashi,; Höhfeld, Jörg,; Ino, Yoko,; Kamp, Roza Maria,; Kazamaki, Reiko,; Kiefhaber, Thomas,; Leitner, Alexander,; Lomas, David A.,; Madden, Dean R.,; Makarov, Oleg A.,; Makowski, Lee,; Mileo, Valentina,; Moehle, Kerstin,; Murphy, Peter W.,; Orville, Allen M.,; Parodi, Armando J.,; Reiner, Andreas,; Riemer, Jan,; Robinson, John A.,; Rowland, Elden E.,; Stephani-Kosin, Kunigunde,; Tschesche, Harald,; Tulumello, David V.,; Ulbricht, Anna,; Vagt, Toni,; Volkmann, Gerrit,; Volkmer, Rudolf,; Xu, Shenglan,
• HerausgeberIn: Tschesche, Harald,
• Place / Publishing House: Berlin ;, Boston : : De Gruyter, , [2011]; ©2012
• Year of Publication: 2011
• Language: English
• Physical Description: 1 online resource (352 p.)

This book presents a survey of recent developments in protein biochemistry. Top researchers in the field of protein biochemistry describe modern methods to address the challenges of protein purification by three-phase partitioning, and their folding and degradation by the functions of chaperones. Th...

Table of Contents

• Frontmatter
• Preface
• Editor. List of contributing authors
• Contents
• Abbreviations
• Acknowledgements
• 1 Three-phase partitioning
• 2 Folding and degradation functions of molecular chaperones
• 3 Membrane protein folding in detergents
• 4 Glycoprotein-folding quality control in the endoplasmic reticulum
• 5 Conformational dynamics in peptides and proteins studied by triplet-triplet energy transfer
• 6 Protein import into the intermembrane space of mitochondria
• 7 On-membrane identification of gel-resolved proteins by matrix-assisted laser desorption ionization mass spectrometry (MALDI-MS)
• 8 Analysis of protein complexes using chemical cross-linking and mass spectrometry
• 9 Single-crystal spectroscopy correlated with X-ray crystallography provides complementary perspectives on macromolecular function
• 10 Wide-angle X-ray solution scattering (WAXS)
• 11 Where purity matters: recombinant versus synthetic peptides in beta amyloid formation
• 12 Chemical modifi cation of proteins in living cells
• 13 Proteomics of human bronchoalveolar lavage fluid: discovery of biomarkers of chronic obstructive pulmonary disease (COPD) with difference gel electrophoresis (DIGE ) and mass spectrometry (MS)
• 14 Proteomic analysis of Duchenne muscular dystrophy (DMD)
• 15 Target-oriented peptide arrays in a palliative approach to cystic fibrosis (CF)
• 16 Probing protein dynamics in vivo using backbone cyclization: bacterial acyl carrier protein as a case study
• 17 The protein epitope mimetic approach to protein-protein interaction inhibitors
• 18 The structural biology of α1-antitrypsin deficiency and the serpinopathies
• Index