Biomarkers of Inflammation and Intestinal Mucosa Pathology in Celiac Disease.

• Superior document: Linköping University Medical Dissertations Series ; v.1672
• Author: Gustafsson Bragde, Hanna.
• Place / Publishing House: Linköping : : Linkopings Universitet,, 2019.; {copy}2019.
• Year of Publication: 2019
• Edition: 1st ed.
• Language: English
• Series: Linköping University Medical Dissertations Series
• Physical Description: 1 online resource (92 pages)

Table of Contents

Intro -- Abstract -- Biomarkörer vid celiaki -- List of publications -- Abbreviations -- Contents -- Introduction -- Celiac disease -- Epidemiology -- Genetics -- Environmental factors -- Effects of celiac disease in the small intestine -- Intestinal mucosa -- Gluten -- Pathogenesis -- Effects of celiac disease in blood -- Extra-intestinal manifestations of celiac disease -- Celiac disease diagnostics, treatment, and follow-up -- Histopathology -- Antibody levels -- HLA typing -- Current recommended diagnostic flow -- Celiac disease treatment -- Celiac disease follow-up -- Conditions with intestinal symptoms similar to celiac disease -- Gene expression -- Biomarkers for celiac disease -- Methods - general overview -- RNA and DNA isolation -- Real-time PCR and TaqMan technology -- Relative quantitative real-time PCR -- Massive parallel sequencing -- RNA sequencing -- Enzyme-linked immunosorbent assay -- Luminex xMAP technology -- Genotyping -- Genotyping using a sequence-specific primer PCR method -- Genotyping using real-time PCR and TaqMan probes -- Thesis aims -- Methods - study specific -- Study subjects and groups -- Biopsy and blood sampling -- Statistical methods and tools -- Paper I -- Sample preparations -- Gene expression analysis -- Statistical analysis -- Paper II -- Sample preparations -- Gene expression analysis -- Protein quantification -- Genotyping -- Statistical analysis -- Paper III -- Sample preparations -- Gene expression analysis -- Genotyping -- Statistical analysis -- Paper IV -- Sample preparations -- Gene expression analysis -- Genotyping -- Statistical analysis -- Results and analysis -- Papers I and III - Gene expression analysis in small intestinal biopsies -- Papers II and IV - RNA and protein levels in blood -- Discussion -- Conclusions -- Thesis contribution and future perspectives -- Acknowledgements -- References.