Alternatives to Animal Testing : : Proceedings of Asian Congress 2016.

• Author: Kojima, Hajime.
• TeilnehmendeR: Seidle, Troy.; Spielmann, Horst.
• Place / Publishing House: Singapore : : Springer Singapore Pte. Limited,, 2018.; ©2019.
• Year of Publication: 2018
• Edition: 1st ed.
• Language: English
• Physical Description: 1 online resource (136 pages)

Table of Contents

Intro -- Contents -- Zebrafish, Danio Rerio as a Replacement Alternative Model Useful in CKDu Experiments -- Abstract -- Introduction -- The Concept of Replacement Alternative -- Scientific Importance of Zebrafish (Danio Rerio) -- Scientific Attributes of Zebrafish -- Introduction of Zebrafish Model to Sri Lanka -- Materials and Methods -- Comprehensive Training on Zebrafish Model -- Results -- Discussion and Conclusion -- Scientific Applicability of Zebrafish Model in the Sri Lankan Context -- Acknowledgments -- References -- Testing Method Development and Validation for in Vitro Skin Irritation Testing (SIT) by Using Reconstructed Human Epidermis (RhE) Skin Equivalent - EPiTRI® -- Abstract -- Introduction -- Materials and Methods -- EPiTRI® Reconstructed Human Epidermis -- Selection of Test Substances -- Cell Viability Measurement by MTT Reduction -- In Vitro Skin Irritation Test (SIT) -- Prediction Model -- Statistical Analysis -- Results and Discussions -- Change of TER and Structural Morphology during the Reconstruction of EPiTRI® -- Quality Control of EPiTRI® -- Protocol Refinements for the Skin Irritation Test of EPiTRI® -- Modification of Washing Method for SIT -- Chemical Exposure Time -- Pre-incubation Time and Post-incubation Volume -- Conclusion -- References -- Development the Technique for the Preparation and Characterization of Reconstructed Human Epidermis (RHE) -- Abstract -- Back Ground -- Structure of Skin Networks -- Epidermis -- Dermis -- Keratinocytes Cells -- Melanocytes Cell -- Fibroblasts Cell -- Reconstruct Ed Human Epiderm is (Rhe) -- Skin Irritation Test -- Examination of Histology Network with Hematoxyline - Eosin Determination -- Immunohistochemical (IHC) -- Result and Discussion -- Cell Culture of Keratinocytes, Melanocytes and Fibroblasts -- Making of Feeder Layer (Dermis Eqivalen).

Making of RHE Tissue (Reconstructed Human Epidermis) -- Measuring of Cell Life Using MTT Method -- Making Histology Preparations of Reconstructed Human Epiderm Using Staining of Hematoxylin-Eosin (HE) -- Immunohistochemical (IHC) Examination of RHE Skin Tissue Using Cytokeratin 10 Antibody Marker -- Conclusion -- References -- Alternative Research (3Rs) in the World, Asia and Japan -- The World -- Asia -- Japan -- References -- Approaches to Reducing Animal Use for Acute Toxicity Testing: Retrospective Analyses of Pesticide Data -- Abstract -- Introduction -- Materials and Methods -- Data Quality Evaluation -- Categorization of Data -- Analyses -- Results -- Distribution of Substances Among Hazard Categories -- EPA Hazard Categories -- GHS Hazard Categories -- Concordance of Oral and Dermal Hazard Classifications -- Discussion -- Disclaimer -- Acknowledgements -- References -- Progress in Eliminating One-Year Dog Studies for the Safety Assessment of Pesticides -- Abstract -- Introduction and Objective -- Review of Regulatory Data not in the Public Domain -- Results of the Studies Conducted in Germany -- Results of the Studies Conducted by the US EPA -- Relevance of the One-Year Dog Study in Assessing Human Health Risks for Registration of Pesticides. An Update to Include Pesticides Registered in Japan -- Discussion -- References -- Cosmetic Regulation and Alternatives to Animal Experimentation in India -- Abstract -- Introduction -- History of Ban of Animal Testing of Cosmetics in EU -- Cosmetic Regulation in India -- Ban on Use of Animals in Cosmetic Testing -- Prohibition of Import of Cosmetics Tested on Animals -- Conclusion -- Acknowledgments -- References -- Guidance on the Use of Alternative Test Methods for the Safety Assessment of Cosmetics and Quasi-drugs -- Abstract -- MHLW Updates -- JaCVAM Update.

Guidance for Alternative to Animal Testing -- References -- Alternatives and Refinement for Animal Experimentation in Cancer Research -- Abstract -- Introduction -- Alternatives in Cancer Research -- 3R's -- CPCSEA -- Refinement in Cancer Research -- Conclusion -- References -- 3Rs in Quality Control of Human Vaccines: Opportunities and Barriers -- Abstract -- Animal Use in Vaccine Quality Control -- 3Rs History and Vaccine Context -- 3Rs Successes -- Barriers to 3Rs -- Perspectives for the Future -- Concluding Remarks -- References -- The Use of Adverse Outcome Pathways (AOPs) to Support Chemical Safety Decisions Within the Context of Integrated Approaches to Testing and Assessment (IATA) -- Abstract -- Introduction -- The Adverse Outcome Pathway (AOP) Framework -- The Use of AOPs to Support Hazard and Risk Assessment -- AOP-Supported IATA Case Study: Skin Sensitization -- Summary -- References -- Mechanism-Based Evaluation System for Hepato- and Nephrotoxicity or Carcinogenicity Using Omics Technology -- Abstract -- Introduction -- Chemicals, Animal Test and Microarray Analysis -- AOP-Based Mechanism of Hepatotoxicity Suggested by Case Study with Carbon Tetrachloride -- AOP-Based Mechanism of Nephrotoxicity Suggested by Case Study with Cisplatin -- Detection System for Hepato- and Nephrotoxicity -- Prediction System for Hepatic and Renal Carcinogenicity: CARCINOscreen® -- Concluding Remarks -- Acknowledgement -- References -- Alternative Methods for Developmental Toxicity Testing Using Mouse ESCs -- Abstract -- Introduction -- Materials and Methods -- Results -- Discussion -- Acknowledgement -- References -- Futuristic Approach to Alternative Model Organisms: Hydra Stakes Its Claim -- Abstract -- Introduction -- Hydra-Systematics -- Physiology and Anatomy -- Reproduction -- Regeneration -- End Point Analysis.

Morphological Observation in Hydra Following Exposure to Chemicals -- Methodology -- Impact of Chemicals on Hydra Growth Rate -- Methodology -- Impact of Chemicals on Hydra's Regeneration Ability -- Methodology -- Impact of Chemicals on Hydra Cell Cycle -- Methodology -- Assessment of Cell Death in Hydra by ROS Generation and Apoptosis -- Determination of ROS Generation in Hydra by H2-DCFDA Staining -- Determination of Apoptosis by Acridine Orange and DAPI Staining -- Acridine Orange Staining -- 4′-6-Diamidino-2-Phenylindole (DAPI) Staining -- Genotoxicity Assessment in Hydra Following Chemical Exposure -- Methodology -- Transcriptional Alterations in Hydra Following Chemicals Exposure -- Conclusion -- Acknowledgments -- References -- The Lush Prize and Young Researcher Asia Awards 2016 -- Abstract -- Introduction -- The Lush Prize Categories -- The Black Box Prize -- The Lush Prize Is a '1R' Initiative -- Launch of Lush Prize 'Young Researcher Asia' Awards in 2016 -- Projects Funded by the Lush Prize to Date -- References -- Author Index.