Pre-Field Screening Protocols for Heat-Tolerant Mutants in Rice.

• Author: Sarsu, Fatma.
• TeilnehmendeR: Ghanim, Abdelbagi M. A.; Das, Priyanka.; Bahuguna, Rajeev N.; Kusolwa, Paul Mbogo.; Ashraf, Muhammed.; Singla-Pareek, Sneh L.; Pareek, Ashwani.; Forster, Brian P.; Ingelbrecht, Ivan.
• Place / Publishing House: Cham : : Springer International Publishing AG,, 2018.; Ã2018.
• Year of Publication: 2018
• Edition: 1st ed.
• Language: English
• Physical Description: 1 online resource (51 pages)

Table of Contents

• Intro
• Preface
• Acknowledgements
• Executive Summary
• Contents
• Chapter 1: General Introduction
• 1.1 Background Analysis
• 1.2 Physiology and Genetics of Heat Tolerance in Rice
• 1.3 Physiological and Biochemical Heat Stress Indicators in Rice
• 1.4 Breeding for Heat Tolerance in Rice
• Chapter 2: Screening Protocols for Heat Tolerance in Rice at the Seedling and Reproductive Stages
• 2.1 Background Analysis
• 2.2 Screening Protocol for Heat Tolerance in Rice at the Seedling Stage
• 2.2.1 Plant Materials
• 2.2.2 Screening for Heat Tolerance Using Hydroponics
• 2.2.2.1 Seedling Establishment in Hydroponics
• 2.2.2.2 Care Required During Plant Growth in Hydroponics
• 2.2.3 Screening for Heat Tolerance in Soil Using Pots
• 2.2.4 Heat Treatment and Recovery of Seedlings
• 2.2.5 Assessment of Heat Tolerance at the Seedling Stage
• 2.2.6 Heat Tolerance Index (HTI)
• 2.2.7 Recovery Stage After Heat Treatment
• 2.3 Screening Protocol for Heat Tolerance in Rice at the Flowering Stage
• 2.3.1 Germination and Seedling Establishment in Hydroponics and Pots
• 2.3.2 Heat Treatment at the Flowering Stage
• 2.3.3 Screening for Heat Tolerance at the Flowering Stage
• Chapter 3: Validation of Screening Protocols for Heat Tolerance in Rice
• 3.1 Background Analysis
• 3.2 Validation of Physiological and Biochemical Indicators
• 3.2.1 Physiological and Biochemical Characterization of Heat Tolerant Lines at the Seedling Stage
• 3.2.2 Physiological and Biochemical Characterization of Heat Tolerant Lines at the Flowering Stage
• 3.2.3 Methods
• 3.2.3.1 Electrolyte Leakage Measurement
• 3.2.3.2 Lipid Peroxidation Assay
• 3.2.3.3 Measurement of Total Proteins
• 3.2.3.4 Antioxidant Enzyme Activity Assay
• 3.3 Validation Protocols of Rice Heat Tolerance Under Field Conditions
• Chapter 4: Conclusion.
• Correction to: Pre-Field Screening Protocols for Heat-Tolerant Mutants in Rice
• References.