Methodologies for metabolomics : experimental strategies and techniques / / edited by Norbert W. Lutz, Jonathan V. Sweedler, Ron A. Wevers.

• Author: ProQuest (Firm)
• TeilnehmendeR: Lutz, Norbert, 1952-; Sweedler, Jonathan V.; Wevers, Ron, 1951-; ProQuest (Firm)
• Year of Publication: 2013
• Language: English
• Physical Description: xii, 627 p. :; ill. (some col.)

"While most of the focus in "omics" science over the past decade has been on sequencing the human genome [1] or annotating the human proteome [2], there is another equally important component of the human body that has, until recently, been largely overlooked: the human metabolome. The human metabolome can be thought of as the complete collection of small molecule metabolites found in our bodies. These small molecules include such chemical entities as peptides, amino acids, nucleic acids, carbohydrates, organic acids, vitamins, minerals, food additives, drugs and just about any other chemical (with a molecular weight 1500 Da) that can be used, ingested or synthesized by humans. Metabolites act as the bricks and mortar of our cells. They serve as the building blocks for all of our macromolecules including proteins, RNA, DNA, carbohydrates, membranes and all other biopolymers that give our cells their structure and integrity. Metabolites also act as the fuel for all cellular processes, the buffers to help tolerate environmental insults and the messengers for most intra- and intercellular events. Together with the genome and the proteome, the human metabolome essentially defines who and what we are"--Provided by publisher.

Table of Contents

Machine generated contents note: 1. Exploring the human metabolome by NMR spectroscopy and mass spectroscopy David S. Wishart; 2. Methodological requirements for lipidomics research Kui Yang, Michael A. Kiebish, Richard W. Gross; 3. Biological methods for metabolic research Arancha Cebrian, Laura Menchen, Elsa Sanchez-Lopez, Juan Casado-Vela, Santiago Diaz-Moralli, Marta Casceante, Teresa Gomez del Pulgar, and Juan Carlos Lacal; 4. Considerations in sample preparation, collection, and extraction approaches applied in microbial, plant, and mammalian metabolic profiling J. William Allwood, Catherine L. Winder, Warwick B. Dunn, and Royston Goodacre; 5. MS-based methodologies for single-cell metabolite detection and identification Ann M. Knolhoff, Peter Nemes, Stanislav S. Rubakhin; 6. Direct metabolomics from tissues and cells: laser ablation electrospray ionization for small molecule and lipid characterization Akos Vertes, Bindesh Shrestha, Peter Nemes; 7. Bioinformatic approaches to process and annotate high-resolution mass spectrometry data Ralf Weber and Mark Viant; 8. Approaches for natural product detection and stuctural elucidation using mass spectrometry with high mass accuracy Ioanna Ntai and Neil L. Kelleher; 9. Metabolomics using ion mobility mass spectrometry Kimberly A. Kaplan and Herbert Hill; 10. Metabolomics via biomedical mass spectrometry: from sampling to clinical applications Bong Chul Chung and Man Ho Choi; 11. Analytical techniques in metabolomics integrating NMR and chromatography with MS U. Braumann and M. Godejohann; 12. NMR of CSF: the neurometabolome Fanny Mochel; 13. NMR analysis and genetic metabolic disease Udo F. H. Engelke, Angelina Goudswaard, Eva Morava, and Ron Weevers; 14. NMR methods for metabolomic investigation of amniotic fluid Ana M. Gil and Goncalo Graca; 15. Chemometric methods in NMR-based body fluid analysis Ron Wehrens and Udo Engelke; 16. Lipid profiling in health and disease Christina E. Kostara and Eleni T. Bairaktari; 17. NMR-based saliva metabolomics Hanne Christine Bertram and Morten Rahr Clausen; 18. Reproducible sample preparation and spectrum acquistion techniques for metabolic profiling of human tissues by H HR-MAS NMR Marital Pitto, F. M. Moussallieh, A. Imperiale, M. A. Benahmed, J. Detour, J. P. Bellocq, I. J. Namer, K. Elbayed; 19. MR spectroscopy in investigating the cancer metabolome in preclinical model systems Marie-France Penet, Zaver M. Bhujwalla, and Kristine Glunde; 20. Metabolomic magnetic resonance spectroscopy of human tissue: a comparison of in vivo and HR-MAS ex vivo techniques Geoffery S. Payne, Yuen-Li Chung, and Martin O. Leach; 21. Phospholipidomics by P NMR spectorcopy of tissue extracts Norbert W. Lutz and Patrick J. Cozzone; 22. C NMR for analysis of metabolic pathways Craig R. Malloy, Elizabeth Maher, Issac Marin-Valencia, Bruce Mickey, Ralph J. DeBerardinis, and A. Dean Sherry; 23. Hyperpolarized NMR spectroscopy - a new method for metabolomic research Ralph E. Hurd, Yi-Fen Yen, and Albert Chen; 24. Assignment strategies for NMR resonances in metabolomics research Teresa W. M. Fan and Andrew N. Lane.